Targeting the lipid membrane to prevent viral entry: the case of a C18-alkylated EGCG compound
Anno: 2026
Rivista: CHEMICO-BIOLOGICAL INTERACTIONS
Autori/autrici: Minnelli, Cristina; Caucci, Sara; Galeazzi, Roberta; Laudadio, Emiliano; Romagnoli, Elena; Frontini, Andrea; Rao, Loredana; Notarstefano, Valentina; Armeni, Tatiana; Romaldi, Brenda; Menzo, Stefano; Mobbili, Giovanna
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/357113
Citazioni:
Targeting the lipid membrane to prevent viral entry: the case of a C18-alkylated EGCG compound / Minnelli, C., Caucci, S., Galeazzi, R., Laudadio, E., Romagnoli, E., Frontini, A., Rao, L., Notarstefano, V., Armeni, T., Romaldi, B., Menzo, S., Mobbili, G.. - In: CHEMICO-BIOLOGICAL INTERACTIONS. - ISSN 0009-2797. - ELETTRONICO. - 430:111971(2026). [10.1016/j.cbi.2026.111971]
Abstract:
Viruses depend on host cell membrane trafficking machinery for most stages of their life cycle, including viral entry, assembly, and immune evasion. Accordingly, alteration in membrane lipid composition and organization may impair viral access to host cells. In this work, we synthesized a series of ...lipophilic derivatives of Epigallocatechin-3-gallate (EGCG) and evaluated their SARS-CoV-2 inhibitory activity in an in vitro model. Among them, EGCG-C18, a 4″-octadecyl EGCG derivative obtained by regio-selective alkylation of the natural polyphenol resulted as the most promising drug candidate. The localization of EGCG-C18 within the cell membrane was assessed by Fourier Transform InfraRed and Raman Microspectroscopy (FTIRM and RMS), while its interaction with monosialotetrahexosylganglioside (GM1), a glycosphingolipid present in specific microdomains of the plasma membrane, known as lipid rafts, was investigated using Molecular Dynamics (MD) simulation, flow cytometry, and confocal microscopy. EGCG-C18 seems to prevent viruses from entering cells by directly impacting the organization of GM1. In addition, we demonstrated that EGCG-C18 treatment decreases EGFR activation, introducing other potential actions of this compound. Finally, EGCG-C18 was evaluated against Herpes Simplex, Influenza B, and HIV-1, to test the hypothesis of a broad-spectrum antiviral activity, potentially driven by its ability to reorganize lipid raft components.
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Diffusion of OXA-48- and NDM-5-producing Klebsiella pneumoniae ST383 clone in Central Italy
Anno: 2026
Rivista: JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
Autori/autrici: D'Achille, Gloria; Cotoloni, Giulia; Nunzi, Ilaria; Brescini, Lucia; Fioriti, Simona; Armiento, Maria; Pocognoli, Antonella; Giovanetti, Eleonora; Paoletti, Claudia; Menzo, Stefano; Brenciani, Andrea; Mingoia, Marina; Morroni, Gianluca
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/361592
Citazioni:
Diffusion of OXA-48- and NDM-5-producing Klebsiella pneumoniae ST383 clone in Central Italy / D'Achille, G., Cotoloni, G., Nunzi, I., Brescini, L., Fioriti, S., Armiento, M., Pocognoli, A., Giovanetti, E., Paoletti, C., Menzo, S., Brenciani, A., Mingoia, M., Morroni, G.. - In: JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY. - ISSN 0305-7453. - 81:1(2026). [10.1093/jac/dkaf397]

Autochthonous dengue outbreak in Marche Region, Central Italy, August to October 2024
Anno: 2024
Rivista: EUROSURVEILLANCE
Autori/autrici: Sacco, Chiara; Liverani, Augusto; Venturi, Giulietta; Gavaudan, Stefano; Riccardo, Flavia; Salvoni, Giovanna; Fortuna, Claudia; Marinelli, Katia; Marsili, Giulia; Pesaresi, Alessia; Grané, Carla Molina; Mercuri, Irene; Manica, Mattia; Caucci, Sara; Morelli, Daniela; Sebastianelli, Lolita; Marcacci, Maurilia; Ferraro, Federica; Di Luca, Marco; Pascucci, Ilaria; Merakou, Christina; Duranti, Anna; Pati, Ilaria; Lombardini, Letizia; Fiacchini, Daniel; Filipponi, Giorgio; Maraglino, Francesco; Palamara, Anna Teresa; Poletti, Piero; Pezzotti, Patrizio; Filippetti, Fabio; Merler, Stefano; Del Manso, Martina; Menzo, Stefano
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/337556
Citazioni:
Autochthonous dengue outbreak in Marche Region, Central Italy, August to October 2024 / Sacco, C., Liverani, A., Venturi, G., Gavaudan, S., Riccardo, F., Salvoni, G., Fortuna, C., Marinelli, K., Marsili, G., Pesaresi, A., Grané, C.M., Mercuri, I., Manica, M., Caucci, S., Morelli, D., Sebastianelli, L., Marcacci, M., Ferraro, F., Di Luca, M., Pascucci, I., et al.. - In: EUROSURVEILLANCE. - ISSN 1560-7917. - 29:47(2024). [10.2807/1560-7917.ES.2024.29.47.2400713]
Abstract:
Between August and 28 October 2024, 199 autochthonous cases of dengue virus serotype 2 were notified in the city of Fano, central Italy. We describe the ongoing epidemiological and microbiological investigation and public health measures implemented to contain the outbreak. The high transmissibility... and the extension of the outbreak suggest that dengue should be expected in temperate regions during favourable seasons, highlighting the need for heightened awareness among healthcare providers and the public to ensure timely detection and response.
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Epidemiological impact of human adenovirus as causative agent of respiratory infections: An Italian multicentre retrospective study, 2022-2023
Anno: 2024
Rivista: JOURNAL OF INFECTION AND CHEMOTHERAPY
Autori/autrici: Giardina, Federica A M; Pellegrinelli, Laura; Novazzi, Federica; Vian, Elisa; Biscaro, Valeria; Russo, Cristina; Ranno, Stefania; Pagani, Elisabetta; Masi, Elisa; Tiberio, Claudia; Esposito, Martina; Uceda Renteria, Sara; Callegaro, Annapaola; Piccirilli, Giulia; Lazzarotto, Tiziana; Rovida, Francesca; Galli, Cristina; Lalle, Eleonora; Maggi, Fabrizio; Mancini, Nicasio; Acciarri, Carla; Menzo, Stefano; Colacicco, Anna Maria; Scarasciulli, Maria; Piralla, Antonio; Baldanti, Fausto; Pariani, Elena
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/337557
Citazioni:
Epidemiological impact of human adenovirus as causative agent of respiratory infections: An Italian multicentre retrospective study, 2022-2023 / Giardina, F.A.M., Pellegrinelli, L., Novazzi, F., Vian, E., Biscaro, V., Russo, C., Ranno, S., Pagani, E., Masi, E., Tiberio, C., Esposito, M., Uceda Renteria, S., Callegaro, A., Piccirilli, G., Lazzarotto, T., Rovida, F., Galli, C., Lalle, E., Maggi, F., Mancini, N., et al.. - In: JOURNAL OF INFECTION AND CHEMOTHERAPY. - ISSN 1341-321X. - 30:11(2024), pp. 1097-1103. [10.1016/j.jiac.2024.07.017]
Abstract:
Human adenoviruses are the causative agents of 5–7% of viral respiratory infections, mainly caused by species B and C. They can infect all age groups, but children are usually at high risk of infections. Adenovirus epidemiology is well documented in East-Asian countries but little is known abo...ut adenovirus circulation in Europe in recent years. This multicentre retrospective study aimed to investigate the circulation and molecular epidemiology of hAdVs. This surveillance collected a total of 54463 respiratory specimens between January 1, 2022 and June 20, 2023 were tested for the presence of respiratory viruses. Our results showed that adenovirus was detected in 6.6 % of all cases of acute respiratory infection included in the study and the median age of positive patients was 3 years, with male children in 1–2 years age group being the most affected. 43.5 % of adenovirus cases were co-infected with at least one other respiratory virus, and rhinovirus was co-detected in 54 % of cases. Genotyping of adenovirus allowed the identification of 6 different genotypes circulating in Italy, among which type B3 was the most frequently detected.
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Multicenter epidemiological investigation and genetic characterization of respiratory syncytial virus and metapneumovirus infections in the pre-pandemic 2018-2019 season in northern and central Italy
Anno: 2023
Rivista: CLINICAL AND EXPERIMENTAL MEDICINE
Autori/autrici: Pierangeli, Alessandra; Piralla, Antonio; Uceda Renteria, Sara; Giacomel, Giovanni; Lunghi, Giovanna; Pagani, Elisabetta; Giacobazzi, Elisabetta; Vian, Elisa; Biscaro, Valeria; Piccirilli, Giulia; Lazzarotto, Tiziana; Menzo, Stefano; Ferreri, Monica Lucia; Novazzi, Federica; Petrarca, Laura; Licari, Amelia; Ferrari, Guglielmo; Oliveto, Giuseppe; Antonelli, Guido; Binda, Sandro; Galli, Cristina; Pellegrinelli, Laura; Pariani, Elena; Baldanti, Fausto
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/324991
Citazioni:
Multicenter epidemiological investigation and genetic characterization of respiratory syncytial virus and metapneumovirus infections in the pre-pandemic 2018-2019 season in northern and central Italy / Pierangeli, A., Piralla, A., Uceda Renteria, S., Giacomel, G., Lunghi, G., Pagani, E., Giacobazzi, E., Vian, E., Biscaro, V., Piccirilli, G., Lazzarotto, T., Menzo, S., Ferreri, M.L., Novazzi, F., Petrarca, L., Licari, A., Ferrari, G., Oliveto, G., Antonelli, G., Binda, S., et al.. - In: CLINICAL AND EXPERIMENTAL MEDICINE. - ISSN 1591-9528. - 23:6(2023), pp. 2725-2737. [10.1007/s10238-022-00973-3]
Abstract:
Respiratory syncytial virus (RSV) and human metapneumovirus (HMPV) cause a high burden of disease, particularly in children and the elderly. With the aim to add knowledge on RSV and HMPV infections in Italy, a prospective, multicenter study was conducted by eight centers of the Working Group on Resp...iratory Virus Infections (GLIViRe), from December 2018-April 2019. Weekly distribution and patients' demographic and clinical data were compared in 1300 RSV and 222 HMPV-positive cases. Phylogenetic analysis of the G-glycoprotein coding region was performed to characterize circulating strains. RSV positivity ranged from 6.4% in outpatients of all ages to 31.7% in hospitalized children; HMPV positivity was 4-1.2% with no age-association. RSV season peaked in February and ended in mid-April: HMPV circulation was higher when RSV decreased in early spring. RSV was more frequent in infants, whereas HMPV infected comparatively more elderly adults; despite, their clinical course was similar. RSV-B cases were two-thirds of the total and had similar clinical severity compared to RSV-A. Phylogenetic analysis showed the circulation of RSV-A ON1 variants and the predominance of RSV-B genotype BA10. HMPV genotype A2c was the prevalent one and presented insertions of different lengths in G. This first multicenter Italian report on seasonality, age-specific distribution, and clinical presentation of RSV and HMPV demonstrated their substantial disease burden in young patients but also in the elderly. These data may provide the basis for a national respiratory virus surveillance network.
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Rapid monitoring of SARS-CoV-2 variants of concern through high-resolution melt analysis
Anno: 2023
Rivista: SCIENTIFIC REPORTS
Autori/autrici: Diotallevi, Aurora; Buffi, Gloria; Barocci, Simone; Ceccarelli, Marcello; Bencardino, Daniela; Andreoni, Francesca; Orlandi, Chiara; Ferri, Marilisa; Vandini, Daniela; Menzo, Stefano; Carlotti, Eugenio; Casabianca, Anna; Magnani, Mauro; Galluzzi, Luca
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/348651
Citazioni:
Rapid monitoring of SARS-CoV-2 variants of concern through high-resolution melt analysis / Diotallevi, A., Buffi, G., Barocci, S., Ceccarelli, M., Bencardino, D., Andreoni, F., Orlandi, C., Ferri, M., Vandini, D., Menzo, S., Carlotti, E., Casabianca, A., Magnani, M., Galluzzi, L.. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - 13:(2023). [10.1038/s41598-023-48929-1]
Abstract:
The current global pandemic of COVID-19 is characterized by waves of infection due to the emergence of new SARS-CoV-2 variants carrying mutations on the Spike (S) protein gene. Since autumn 2020 many Variants of Concern (VOC) have been reported: Alpha/B.1.1.7, Beta/B.1.351, Gamma/P.1, Delta/B.1.617....2, Omicron/B.1.1.529, and sublineages. Surveillance of genomic variants is currently based on whole-genome sequencing (WGS) of viral genomes on a random fraction of samples positive to molecular tests. WGS involves high costs, extended analysis time, specialized staff, and expensive instruments compared to a PCR-based test. To rapidly identify the VOCs in positive samples, six assays based on real-time PCR and high-resolution melting (HRM) were designed on the S gene and applied to 120 oro/nasopharyngeal swab samples collected from October 2020 to June 2022 (106 positive and 14 negative samples). Overall, the assays showed 100% specificity and sensitivity compared with commercial PCR tests for COVID-19. Moreover, 104 samples out of 106 (98.1%) were correctly identified as follows: 8 Wuhan (wild type), 12 Alpha, 23 Delta, 46 Omicron BA.1/BA.1.1, 15 Omicron BA.2/BA.4/BA.5. With our lab equipment, about 10 samples can be processed every 3 h at the cost of less than € 10 ($ 10.60) per sample, including RNA extraction. The implementation of this approach could help local epidemiological surveillance and clinical decision-making.
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Serological response after anti-SARS-CoV-2 BNT162b2 vaccine in IBD patients on biological therapy: a monocentric case-control study
Anno: 2023
Rivista: MINERVA GASTROENTEROLOGY
Autori/autrici: Montori, Michele; Martini, Francesco; De Blasio, Federico; Buono, Tiziana; Quatraccioni, Claudia; Guardati, Paola; Calzolari, Manuela; Rossi, Chiara; Bendia, Emanuele; Menzo, Stefano; Valenza, Carmine; Pellicano, Rinaldo; Maroni, Luca; Benedetti, Antonio
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/328255
Citazioni:
Serological response after anti-SARS-CoV-2 BNT162b2 vaccine in IBD patients on biological therapy: a monocentric case-control study / Montori, M., Martini, F., De Blasio, F., Buono, T., Quatraccioni, C., Guardati, P., Calzolari, M., Rossi, C., Bendia, E., Menzo, S., Valenza, C., Pellicano, R., Maroni, L., Benedetti, A.. - In: MINERVA GASTROENTEROLOGY. - ISSN 2724-5985. - 69:2(2023). [10.23736/S2724-5985.22.03134-5]
Abstract:
BACKGROUND: Inflammatory bowel disease (IBD) patients on biological therapy are receiving vaccines against severe acute respiratory syndrome Coronavirus 2 (SARS-CoV-2). However, it is unclear if IBD therapy could influence the response to this vaccine. In a case-control study, we assessed the antibo...dy profiling after anti-SARS-CoV-2 BNT162b2 vaccine in IBD patients on biological therapy. METHODS: We analyzed seroprevalence and antibody titer, after 14 weeks from the first BNT162b2 vaccine dose, in IBD patients on biological therapy and health care workers (HCWs). In IBD patients, medical history and disease data were recorded.RESULTS: Eighty-two subjects were enrolled in this study. Among them, 40 were IBD patients on biological therapy and 42 were HCWs. All subjects developed an IgG anti-Spike antibody titer above the cut-off. IBD patients on biological therapy developed a lower antibody titer than HCWs (P<0.00001). No differences were reported in patients who received at least one dose of the vaccine within a period of 7 days from the last biological drug administration, compared to all oth-er IBD patients. A difference was found between patients who were on concomitant immunosuppressive therapy and pa-tients on sole biological therapy (P=0.0287). Patients with presence of any sign of disease activity (clinical, endoscopic or laboratory) showed a higher development of antibody titer compared to those in complete disease remission (P=0.0468).CONCLUSIONS: Our data indicate that in IBD patients, treatment with biological therapies do not affect the seropreva-lence but leads to a lower antibody titer development after anti-SARS-CoV-2 BNT162b2 vaccine.(Cite this article as: Montori M, Martini F, De Blasio F, Buono T, Quatraccioni C, Guardati P, et al. Serological response after anti-SARS-CoV-2 BNT162b2 vaccine in IBD patients on biological therapy: a monocentric case-control study. Minerva Gastroenterol 2023;69:268-76. DOI: 10.23736/S2724-5985.22.03134-5)
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Rapid monitoring of SARS-CoV-2 variants of concern through high-resolution melt analysis
Anno: 2023
Rivista: SCIENTIFIC REPORTS
Autori/autrici: Diotallevi, Aurora; Buffi, Gloria; Barocci, Simone; Ceccarelli, Marcello; Bencardino, Daniela; Andreoni, Francesca; Orlandi, Chiara; Ferri, Marilisa; Vandini, Daniela; Menzo, Stefano; Carlotti, Eugenio; Casabianca, Anna; Magnani, Mauro; Galluzzi, Luca
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/327696
Citazioni:
Rapid monitoring of SARS-CoV-2 variants of concern through high-resolution melt analysis / Diotallevi, A., Buffi, G., Barocci, S., Ceccarelli, M., Bencardino, D., Andreoni, F., Orlandi, C., Ferri, M., Vandini, D., Menzo, S., Carlotti, E., Casabianca, A., Magnani, M., Galluzzi, L.. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - 13:1(2023). [10.1038/s41598-023-48929-1]
Abstract:
The current global pandemic of COVID-19 is characterized by waves of infection due to the emergence of new SARS-CoV-2 variants carrying mutations on the Spike (S) protein gene. Since autumn 2020 many Variants of Concern (VOC) have been reported: Alpha/B.1.1.7, Beta/B.1.351, Gamma/P.1, Delta/B.1.617....2, Omicron/B.1.1.529, and sublineages. Surveillance of genomic variants is currently based on whole-genome sequencing (WGS) of viral genomes on a random fraction of samples positive to molecular tests. WGS involves high costs, extended analysis time, specialized staff, and expensive instruments compared to a PCR-based test. To rapidly identify the VOCs in positive samples, six assays based on real-time PCR and high-resolution melting (HRM) were designed on the S gene and applied to 120 oro/nasopharyngeal swab samples collected from October 2020 to June 2022 (106 positive and 14 negative samples). Overall, the assays showed 100% specificity and sensitivity compared with commercial PCR tests for COVID-19. Moreover, 104 samples out of 106 (98.1%) were correctly identified as follows: 8 Wuhan (wild type), 12 Alpha, 23 Delta, 46 Omicron BA.1/BA.1.1, 15 Omicron BA.2/BA.4/BA.5. With our lab equipment, about 10 samples can be processed every 3 h at the cost of less than € 10 ($ 10.60) per sample, including RNA extraction. The implementation of this approach could help local epidemiological surveillance and clinical decision-making.
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Adeno-associated virus type 5 infection via PDGFRα is associated with Interstitial lung disease in systemic sclerosis and generates composite peptides and epitopes recognized by the agonistic immunoglobulins present in patients with systemic sclerosis
Anno: 2023
Rivista: ARTHRITIS & RHEUMATOLOGY
Autori/autrici: Moroncini, Gianluca; Svegliati, Silvia; Grieco, Antonella; Cuccioloni, Massimiliano; Mozzicafreddo, Matteo; Paolini, Chiara; Agarbati, Silvia; Spadoni, Tatiana; Amoresano, Angela; Pinto, Gabriella; Chen, Qingxin; Benfaremo, Devis; Tonnini, Cecilia; Senzacqua, Martina; Alizzi, Silvia; Nieto, Karen; Finke, Doreen; Viola, Nadia; Amico, Donatella; Galgani, Mario; Gasparini, Stefano; Zuccatosta, Lina; Menzo, Stefano; Müller, Martin; Kleinschmidt, Juergen; Funaro, Ada; Giordano, Antonio; La Cava, Antonio; Dorfmuller, Peter; Amoroso, Antonio; Pucci, Piero; Pezone, Antonio; Avvedimento, Enrico V; Gabrielli, Armando
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/324693
Citazioni:
Adeno-associated virus type 5 infection via PDGFRα is associated with Interstitial lung disease in systemic sclerosis and generates composite peptides and epitopes recognized by the agonistic immunoglobulins present in patients with systemic sclerosis / Moroncini, G., Svegliati, S., Grieco, A., Cuccioloni, M., Mozzicafreddo, M., Paolini, C., Agarbati, S., Spadoni, T., Amoresano, A., Pinto, G., Chen, Q., Benfaremo, D., Tonnini, C., Senzacqua, M., Alizzi, S., Nieto, K., Finke, D., Viola, N., Amico, D., Galgani, M., et al.. - In: ARTHRITIS & RHEUMATOLOGY. - ISSN 2326-5191. - 76:4(2023), pp. 620-630. [10.1002/art.42746]
Abstract:
Objectives: The etiopathogenesis of systemic sclerosis (SSc) is unknown. Platelet-derived growth factor receptors (PDGFRs) are overexpressed in SSc patients. Since PDGFRα is targeted of the adeno-associated virus type 5 (AAV5), we investigated whether AAV5 forms a complex with PDGFRα exp...osing epitopes that may induce the immune responses to the virus-PDGFRα complex. Methods: The binding of monomeric human PDGFRα to the AAV5 capsid was analyzed by in silico molecular docking, surface plasmon resonance (SPR), and genome editing of the PDGFRα locus. AAV5 was detected in SSc lungs by in situ hybridization, immunohistochemistry, confocal microscopy, and molecular analysis of bronchoalveolar lavage (BAL). Immune responses to AAV5 and PDGFRα were evaluated by SPR using SSc monoclonal anti-PDGFRα antibodies and Immunoaffinity-purified anti-PDGFRα antibodies from sera of SSc patients. Results: AAV5 was detected in the BAL of 41 out of 66 (62.1%) SSc patients with interstitial lung disease and in 17 of 66 controls (25.75 %; p&lt;0.001). In SSc lungs, AAV5 localized in type II pneumocytes and in interstitial cells. A molecular complex formed of spatially contiguous epitopes of the AAV5 capsid and PDGFRα was identified and characterized. In silico molecular docking analysis and binding to the agonistic anti-PDGFRα antibodies identified spatially contiguous epitopes derived from PDGFRα and AAV5 that interacted with SSc agonistic antibodies to PDGFRα. These peptides were also able to bind total IgG isolated from SSc patients, not from healthy controls. Conclusions: These data link AVV5 with the immune reactivity to endogenous antigens in SSc, and provide a novel element in the pathogenesis of SSc.
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Multicenter epidemiological investigation and genetic characterization of respiratory syncytial virus and metapneumovirus infections in the pre-pandemic 2018–2019 season in northern and central Italy
Anno: 2023
Rivista: CLINICAL AND EXPERIMENTAL MEDICINE
Autori/autrici: Pierangeli, Alessandra; Piralla, Antonio; Uceda Renteria, Sara; Giacomel, Giovanni; Lunghi, Giovanna; Pagani, Elisabetta; Giacobazzi, Elisabetta; Vian, Elisa; Biscaro, Valeria; Piccirilli, Giulia; Lazzarotto, Tiziana; Menzo, Stefano; Ferreri, Monica Lucia; Novazzi, Federica; Petrarca, Laura; Licari, Amelia; Ferrari, Guglielmo; Oliveto, Giuseppe; Antonelli, Guido; Binda, Sandro; Galli, Cristina; Pellegrinelli, Laura; Pariani, Elena; Baldanti, Fausto
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/327697
Citazioni:
Multicenter epidemiological investigation and genetic characterization of respiratory syncytial virus and metapneumovirus infections in the pre-pandemic 2018–2019 season in northern and central Italy / Pierangeli, A., Piralla, A., Uceda Renteria, S., Giacomel, G., Lunghi, G., Pagani, E., Giacobazzi, E., Vian, E., Biscaro, V., Piccirilli, G., Lazzarotto, T., Menzo, S., Ferreri, M.L., Novazzi, F., Petrarca, L., Licari, A., Ferrari, G., Oliveto, G., Antonelli, G., Binda, S., et al.. - In: CLINICAL AND EXPERIMENTAL MEDICINE. - ISSN 1591-9528. - 23:6(2023), pp. 2725-2737. [10.1007/s10238-022-00973-3]
Abstract:
Respiratory syncytial virus (RSV) and human metapneumovirus (HMPV) cause a high burden of disease, particularly in children and the elderly. With the aim to add knowledge on RSV and HMPV infections in Italy, a prospective, multicenter study was conducted by eight centers of the Working Group on Resp...iratory Virus Infections (GLIViRe), from December 2018-April 2019. Weekly distribution and patients' demographic and clinical data were compared in 1300 RSV and 222 HMPV-positive cases. Phylogenetic analysis of the G-glycoprotein coding region was performed to characterize circulating strains. RSV positivity ranged from 6.4% in outpatients of all ages to 31.7% in hospitalized children; HMPV positivity was 4-1.2% with no age-association. RSV season peaked in February and ended in mid-April: HMPV circulation was higher when RSV decreased in early spring. RSV was more frequent in infants, whereas HMPV infected comparatively more elderly adults; despite, their clinical course was similar. RSV-B cases were two-thirds of the total and had similar clinical severity compared to RSV-A. Phylogenetic analysis showed the circulation of RSV-A ON1 variants and the predominance of RSV-B genotype BA10. HMPV genotype A2c was the prevalent one and presented insertions of different lengths in G. This first multicenter Italian report on seasonality, age-specific distribution, and clinical presentation of RSV and HMPV demonstrated their substantial disease burden in young patients but also in the elderly. These data may provide the basis for a national respiratory virus surveillance network.
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SARS-CoV-2 multi-variant rapid detector based on graphene transistor functionalized with an engineered dimeric ACE2 receptor
Anno: 2023
Rivista: NANO TODAY
Autori/autrici: Romagnoli, A.; D'Agostino, M.; Pavoni, E.; Ardiccioni, C.; Motta, S.; Crippa, P.; Biagetti, G.; Notarstefano, V.; Rexha, J.; Perta, N.; Barocci, S.; Costabile, B. K.; Colasurdo, G.; Caucci, S.; Mencarelli, D.; Turchetti, C.; Farina, M.; Pierantoni, L.; La Teana, A.; Al Hadi, R.; Cicconardi, F.; Chinappi, M.; Trucchi, E.; Mancia, F.; Menzo, S.; Morozzo della Rocca, B.; D'Annessa, I.; Di Marino, D.
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/309546
Citazioni:
SARS-CoV-2 multi-variant rapid detector based on graphene transistor functionalized with an engineered dimeric ACE2 receptor / Romagnoli, A., D'Agostino, M., Pavoni, E., Ardiccioni, C., Motta, S., Crippa, P., Biagetti, G., Notarstefano, V., Rexha, J., Perta, N., Barocci, S., Costabile, B.K., Colasurdo, G., Caucci, S., Mencarelli, D., Turchetti, C., Farina, M., Pierantoni, L., La Teana, A., Al Hadi, R., et al.. - In: NANO TODAY. - ISSN 1748-0132. - 48:(2023). [10.1016/j.nantod.2022.101729]
Abstract:
Reliable point-of-care (POC) rapid tests are crucial to detect infection and contain the spread of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). The emergence of several variants of concern (VOC) can reduce binding affinity to diagnostic antibodies, limiting the efficacy of the curre...ntly adopted tests, while showing unaltered or increased affinity for the host receptor, angiotensin converting enzyme 2 (ACE2). We present a graphene field-effect transistor (gFET) biosensor design, which exploits the Spike-ACE2 interaction, the crucial step for SARS-CoV-2 infection. Extensive computational analyses show that a chimeric ACE2-Fragment crystallizable (ACE2-Fc) construct mimics the native receptor dimeric conformation. ACE2-Fc functionalized gFET allows in vitro detection of the trimeric Spike protein, outperforming functionalization with a diagnostic antibody or with the soluble ACE2 portion, resulting in a sensitivity of 20 pg/mL. Our miniaturized POC biosensor successfully detects B.1.610 (pre-VOC), Alpha, Beta, Gamma, Delta, Omicron (i.e., BA.1, BA.2, BA.4, BA.5, BA.2.75 and BQ.1) variants in isolated viruses and patient's clinical nasopharyngeal swabs. The biosensor reached a Limit Of Detection (LOD) of 65 cps/mL in swab specimens of Omicron BA.5. Our approach paves the way for a new and reusable class of highly sensitive, rapid and variant-robust SARS-CoV-2 detection systems.
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Phylogeography and genomic epidemiology of SARS-CoV-2 in Italy and Europe with newly characterized Italian genomes between February-June 2020
Anno: 2022
Rivista: SCIENTIFIC REPORTS
Autori/autrici: Lai, A.; Bergna, A.; Toppo, S.; Morganti, M.; Menzo, S.; Ghisetti, V.; Bruzzone, B.; Codeluppi, M.; Fiore, V.; Rullo, E. V.; Antonelli, G.; Sarmati, L.; Brindicci, G.; Callegaro, A.; Sagnelli, C.; Francisci, D.; Vicenti, I.; Miola, A.; Tonon, G.; Cirillo, D.; Menozzi, I.; Caucci, S.; Cerutti, F.; Orsi, A.; Schiavo, R.; Babudieri, S.; Nunnari, G.; Mastroianni, C. M.; Andreoni, M.; Monno, L.; Guarneri, D.; Coppola, N.; Crisanti, A.; Galli, M.; Zehender, G.; Balotta, C.; Ventura, C.; Schiuma, M.; Lavezzo, E.; Fontana, P.; Bianco, L.; Bertolotti, L.; Manuto, L.; Grazioli, M.; Bianca, F.; Del Vecchio, C.; Franchin, E.; Onelia, F.; Spitaleri, A.; Saluzzo, F.; Lorenzin, G.; Pongolini, S.; Scaltriti, E.; Soliani, L.; Bagnarelli, P.; Turchi, C.; Onofri, V.; Melchionda, F.; Tagliabracci, A.; Burdino, E.; Milia, M. G.; Caligiuri, P.; De Pace, V.; Ricucci, V.; Domnich, A.; Boccotti, S.; Cristina, L. M.; Cascio, G. L.; Rubino, S.; Lai, V.; Rocca, G.; Govoni, R.; Mancuso, G.; Campagna, R.; Mazzuti, L.; Oliveto, G.; Turriziani, O.; Campogiani, L.; Compagno, M.; Coppola, L.; Crea, A. M. A.; De Simone, G.; Di Lorenzo, A.; Ferrari, L.; Iannetta, M.; Malagnino, V.; Mulas, T.; Rossi, B.; Spalliera, I.; Tedde, S.; Teti, E.; Vitale, P.; Zordan, M.; Milano, E.; Lagioia, A.; Gallitelli, R.; Starace, M.; Minichini, C.; Di Fraia, A.; Schioppa, M.; Greco, R.; Gidari, A.; Zazzi, M.; Dragoni, F.; Puma, L. L.; Ronchiadin, S.; Ruggerone, L.; Russignaga, D.
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/308764
Citazioni:
Phylogeography and genomic epidemiology of SARS-CoV-2 in Italy and Europe with newly characterized Italian genomes between February-June 2020 / Lai, A., Bergna, A., Toppo, S., Morganti, M., Menzo, S., Ghisetti, V., Bruzzone, B., Codeluppi, M., Fiore, V., Rullo, E.V., Antonelli, G., Sarmati, L., Brindicci, G., Callegaro, A., Sagnelli, C., Francisci, D., Vicenti, I., Miola, A., Tonon, G., Cirillo, D., et al.. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - 12:1(2022). [10.1038/s41598-022-09738-0]
Abstract:
The aims of this study were to characterize new SARS-CoV-2 genomes sampled all over Italy and to reconstruct the origin and the evolutionary dynamics in Italy and Europe between February and June 2020. The cluster analysis showed only small clusters including < 80 Italian isolates, while most of ...the Italian strains were intermixed in the whole tree. Pure Italian clusters were observed mainly after the lockdown and distancing measures were adopted. Lineage B and B.1 spread between late January and early February 2020, from China to Veneto and Lombardy, respectively. Lineage B.1.1 (20B) most probably evolved within Italy and spread from central to south Italian regions, and to European countries. The lineage B.1.1.1 (20D) developed most probably in other European countries entering Italy only in the second half of March and remained localized in Piedmont until June 2020. In conclusion, within the limitations of phylogeographical reconstruction, the estimated ancestral scenario suggests an important role of China and Italy in the widespread diffusion of the D614G variant in Europe in the early phase of the pandemic and more dispersed exchanges involving several European countries from the second half of March 2020.
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Visceral fat inflammation and fat embolism are associated with lung's lipidic hyaline membranes in subjects with COVID-19
Anno: 2022
Rivista: INTERNATIONAL JOURNAL OF OBESITY
Autori/autrici: Colleluori, G; Graciotti, L; Pesaresi, M; Di Vincenzo, A; Perugini, J; Di Mercurio, E; Caucci, S; Bagnarelli, P; Zingaretti, C; Nisoli, E; Menzo, S; Tagliabracci, A; Ladoux, A; Dani, C; Giordano, A; Cinti, S.
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/297007
Citazioni:
Visceral fat inflammation and fat embolism are associated with lung's lipidic hyaline membranes in subjects with COVID-19 / Colleluori, G., Graciotti, L., Pesaresi, M., Di Vincenzo, A., Perugini, J., Di Mercurio, E., Caucci, S., Bagnarelli, P., Zingaretti, C., Nisoli, E., Menzo, S., Tagliabracci, A., Ladoux, A., Dani, C., Giordano, A., Cinti, S.. - In: INTERNATIONAL JOURNAL OF OBESITY. - ISSN 1476-5497. - ELETTRONICO. - 46:5(2022), pp. 1009-1017. [10.1038/s41366-022-01071-w]
Abstract:
Background: Preliminary data suggested that fat embolism could explain the importance of visceral obesity as a critical determinant of coronavirus disease-2019 (COVID-19). Methods: We performed a comprehensive histomorphologic analysis of autoptic visceral adipose tissue (VAT), lungs and livers of 1...9 subjects with COVID-19 (COVID-19+), and 23 people without COVID-19 (controls). Human adipocytes (hMADS) infected with SARS-CoV-2 were also studied. Results: Although there were no between-group differences in body-mass-index and adipocytes size, a higher prevalence of CD68+ macrophages among COVID-19+ VAT was detected (p = 0.005) and accompanied by crown-like structures presence, signs of adipocytes stress and death. Consistently, human adipocytes were successfully infected by SARS-CoV-2 in vitro and displayed lower cell viability. Being VAT inflammation associated with lipids spill-over from dead adipocytes, we studied lipids distribution by ORO. Lipids were observed within lungs and livers interstitial spaces, macrophages, endothelial cells, and vessels lumen, features suggestive of fat embolism syndrome, more prevalent among COVID-19+ (p &lt; 0.001). Notably, signs of fat embolism were more prevalent among people with obesity (p = 0.03) independently of COVID-19 diagnosis, suggesting that such condition may be an obesity complication exacerbated by SARS-CoV-2 infection. Importantly, all infected subjects’ lungs presented lipids-rich (ORO+) hyaline membranes, formations associated with COVID-19-related pneumonia, present only in one control patient with non-COVID-19-related pneumonia. Importantly, transition aspects between embolic fat and hyaline membranes were also observed. Conclusions: This study confirms the lung fat embolism in COVID-19+ patients and describes for the first time novel COVID-19-related features possibly underlying the unfavorable prognosis in people with COVID-19 and obesity.
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POOR RESPONSE TO FIRST BNT162B2 SARS-COV-2 VACCINE DOSE IN RECIPIENTS OF ALLOGENEIC HEMATOPOIETIC STEM CELL TRANSPLANT
Anno: 2021
Rivista: HAEMATOLOGICA
Autori/autrici: Saraceni, F; Fiorentini, A; More', S; Guerzoni, S; Puglisi, B; Corvaro, B; Menzo, S; Butini, L; Costantini, A; Viola, N; Lotito, Af; Colaneri, Fr; Scortechini, I; Mancini, G; Dubbini, Mv; Federici, I; Offidani, M; Olivieri, A
Lingua: ENG
Classificazione IRIS: 1.5 Abstract in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/296381
Citazioni:
POOR RESPONSE TO FIRST BNT162B2 SARS-COV-2 VACCINE DOSE IN RECIPIENTS OF ALLOGENEIC HEMATOPOIETIC STEM CELL TRANSPLANT / Saraceni, F., Fiorentini, A., More', S., Guerzoni, S., Puglisi, B., Corvaro, B., Menzo, S., Butini, L., Costantini, A., Viola, N., Lotito, A.f., Colaneri, F.r., Scortechini, I., Mancini, G., Dubbini, M.v., Federici, I., Offidani, M., Olivieri, A.. - In: HAEMATOLOGICA. - ISSN 0390-6078. - STAMPA. - 106:(2021), pp. 175-176.
Abstract:
On March 2021, the Italian National COVID-19 vaccination campaign was extended to patients with high risk chronic medical conditions, including recipients of allogeneic stem cell transplant (allo-SCT). In the present study, we prospectively assessed serological response following the first BNT162b2 ...SARS-CoV-2 vaccine dose in recipients of allo-SCT in our centre. Inclusion criteria for the participation in this study included: (1) age above 18 years; (2) allo-SCT for any hematological disease; (3) eligibility for vaccination. Patient serum was collected on day 1 (D1; before the first BNT162b2 dose), and on day 21 (D21; before the second dose of the vaccine). IgG antibodies to the receptor binding domain (RBD) of the S1 subunit of the spike protein of SARS-CoV-2 were analyzed by CMIA assay. The study population included 34 recipients of allo-SCT (22 males/12 females; median age: 59 years, range 28-70 years). Patients characteristics are depicted in Table 1. Thirty-one patients were evaluable for serological assessments on D1 and D21. On D1, only 1 patient with known previous exposure to SARS-CoV-2 had detectable anti-S and anti-N IgG antibodies. After the first dose of vaccine, on D21, 4/31 (13%) patients had detectable anti-S IgG antibodies above the cut-off of 7,1 BAU, excluding the patient who had experienced natural infection. Median anti-S IgG titer of responders was 121,6 BAU (range 32,8-481). Interestingly, all these four patients were receiving tyrosine kinase (TKI) inhibitors for an ongoing cGVHD (Ruxolitinib, 2 pts; Imatinib, 2 pts) at the time of vaccination. Median time from transplant to vaccination was 51 months (range 32-74) in responders, as compared to 10 months (range 4-142) in non responders. In fact, no patients who had undergone allo-SCT from less than 2 years showed an antibody response. Our preliminary data suggest that the first dose of BNT162b2 vaccine leads to production of SARS-CoV-2 IgG antibodies in a minority of recipients of allogeneic stem cell transplant. Further, patients who received vaccination later after transplant seem more likely to show antibody production.
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High prevalence of carbapenem-resistant Klebsiella pneumoniae ST307 recovered from faecal samples in an Italian hospital
Anno: 2021
Rivista: FUTURE MICROBIOLOGY
Autori/autrici: Magi, Gloria; Tontarelli, Federica; Caucci, Sara; Di Sante, Laura; Brenciani, Andrea; Morroni, Gianluca; Giovanetti, Eleonora; Menzo, Stefano; Mingoia, Marina
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/291006
Citazioni:
High prevalence of carbapenem-resistant Klebsiella pneumoniae ST307 recovered from faecal samples in an Italian hospital / Magi, G., Tontarelli, F., Caucci, S., Di Sante, L., Brenciani, A., Morroni, G., Giovanetti, E., Menzo, S., Mingoia, M.. - In: FUTURE MICROBIOLOGY. - ISSN 1746-0913. - STAMPA. - 16:10(2021), pp. 703-711. [10.2217/fmb-2020-0246]
Abstract:
Aim: This study reports the characterization of carbapenem-resistant colonizing strains of K. pneumoniae.Methods: 650 stool samples were screened for carbapenem-resistant K. pneumoniae (CR-Kp). All strains were characterized for antibiotic susceptibility, typing features, main carbapenemases and ext...endedspectrum ß-lactamases. The carbapenemase transferability was assessed by interspecific conjugation. Results: Eighteen CR-Kp were multidrug-resistant; five were KPC-producing. A predominance of ST307 isolates, constituting the predominant cluster by PFGE analysis, was identified (50% were KPC-2 producers). Conjugation data showed the co-transfer of blaKPC-2, blaTEM-1, blaOXA-1, blaCTX-M-15 in a single large pKPN3-like plasmid.Conclusion: Our data pointed out the diversity of colonizing K. pneumoniae strains compared with clinical ones. The predominance of ST307 strains suggested an increased spreading, even in our area, of this high-risk clone.
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Tocilizumab in COVID-19 interstitial pneumonia
Anno: 2021
Rivista: JOURNAL OF INTERNAL MEDICINE
Autori/autrici: Pomponio, G; Ferrarini, A; Bonifazi, M; Moretti, M; Salvi, A; Giacometti, A; Tavio, M; Titolo, G; Morbidoni, L; Frausini, G; Onesta, M; Amico, D; B Rocchi, M L; Menzo, S; Zuccatosta, L; Mei, F; Menditto, V; Svegliati, S; Donati, A; M D'Errico, M; Pavani, M; Gabrielli, A
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/290128
Citazioni:
Tocilizumab in COVID-19 interstitial pneumonia / Pomponio, G., Ferrarini, A., Bonifazi, M., Moretti, M., Salvi, A., Giacometti, A., Tavio, M., Titolo, G., Morbidoni, L., Frausini, G., Onesta, M., Amico, D., B Rocchi, M.L., Menzo, S., Zuccatosta, L., Mei, F., Menditto, V., Svegliati, S., Donati, A., M D'Errico, M., et al.. - In: JOURNAL OF INTERNAL MEDICINE. - ISSN 0954-6820. - ELETTRONICO. - 289:5(2021), pp. 738-746. [10.1111/joim.13231]
Abstract:
Background: Published reports on tocilizumab in COVID-19 pneumonitis show conflicting results due to weak designs or heterogeneity in critical methodological issues. Methods: This open-label trial, structured according to Simon's optimal design, aims to identify factors predicting which patient...s could benefit from anti-IL6 strategies and to enhance the design of unequivocal and reliable future randomized trials. A total of 46 patients with COVID-19 pneumonia needing of oxygen therapy to maintain SO2 &gt; 93% and with recent worsening of lung function received a single infusion of tocilizumab. Clinical and biological markers were measured to test their predictive values. Primary end point was early and sustained clinical response. Results: Twenty-one patients fulfilled pre-defined response criteria. Lower levels of IL-6 at 24 h after tocilizumab infusion (P = 0.049) and higher baseline values of PaO2/FiO2 (P = 0.008) predicted a favourable response. Conclusions: Objective clinical response rate overcame the pre-defined threshold of 30%. Efficacy of tocilizumab to improve respiratory function in patients selected according to our inclusion criteria warrants investigations in randomized trials.
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Weak cross-lineage neutralization by anti sars-cov-2 spike antibodies after natural infection or vaccination is rescued by repeated immunological stimulation
Anno: 2021
Rivista: VACCINES
Autori/autrici: Caucci, S.; Corvaro, B.; Tiano, S. M. L.; Valenza, A.; Longo, R.; Marinelli, K.; Ferreri, M. L.; Spiridigliozzi, P.; Salvoni, G.; Bagnarelli, P.; Menzo, S.
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/303019
Citazioni:
Weak cross-lineage neutralization by anti sars-cov-2 spike antibodies after natural infection or vaccination is rescued by repeated immunological stimulation / Caucci, S., Corvaro, B., Tiano, S.M.L., Valenza, A., Longo, R., Marinelli, K., Ferreri, M.L., Spiridigliozzi, P., Salvoni, G., Bagnarelli, P., Menzo, S.. - In: VACCINES. - ISSN 2076-393X. - 9:10(2021), p. 1124. [10.3390/vaccines9101124]
Abstract:
After over one year of evolution, through billions of infections in humans, SARS-CoV-2 has evolved into a score of slightly divergent lineages. A few different amino acids in the spike proteins of these lineages can hamper both natural immunity against reinfection, and vaccine efficacy. In this stud...y, the in vitro neutralizing potency of sera from convalescent COVID-19 patients and vaccinated subjects was analyzed against six different SARS-CoV-2 lineages, including the latest B.1.617.2 (or Delta variant), in order to assess the cross-neutralization by anti-spike anti-bodies. After both single dose vaccination, or natural infection, the neutralizing activity was low and fully effective only against the original lineage, while a double dose or a single dose of vaccine, even one year after natural infection, boosted the cross-neutralizing activity against different lin-eages. Neither binding, nor the neutralizing activity of sera after vaccination, could predict vaccine failure, underlining the need for additional immunological markers. This study points at the im-portance of the anamnestic response and repeated vaccine stimulations to elicit a reasonable cross-lineage neutralizing antibody response.
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Circulating SARS-CoV-2 variants in Italy, October 2020–March 2021
Anno: 2021
Rivista: VIROLOGY JOURNAL
Autori/autrici: Lai, A.; Bergna, A.; Menzo, S.; Zehender, G.; Caucci, S.; Ghisetti, V.; Rizzo, F.; Maggi, F.; Cerutti, F.; Giurato, G.; Weisz, A.; Turchi, C.; Bruzzone, B.; Ceccherini Silberstein, F.; Clementi, N.; Callegaro, A.; Sagradi, F.; Francisci, D.; Venanzi Rullo, E.; Vicenti, I.; Clementi, M.; Galli, M.; Balotta, C.; Gori, M.; Bagnarelli, P.; Baj, A.; Novazzi, F.; Orsi, A.; Caligiuri, P.; Boccotti, S.; Bellocchi, M. C.; Sarmati, L.; Andreoni, M.; Mancini, N.; Criscuolo, E.; Gallitelli, R.; Testa, S.; Dragoni, F.; Zazzi, M.
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/291802
Citazioni:
Circulating SARS-CoV-2 variants in Italy, October 2020–March 2021 / Lai, A., Bergna, A., Menzo, S., Zehender, G., Caucci, S., Ghisetti, V., Rizzo, F., Maggi, F., Cerutti, F., Giurato, G., Weisz, A., Turchi, C., Bruzzone, B., Ceccherini Silberstein, F., Clementi, N., Callegaro, A., Sagradi, F., Francisci, D., Venanzi Rullo, E., Vicenti, I., et al.. - In: VIROLOGY JOURNAL. - ISSN 1743-422X. - ELETTRONICO. - 18:1(2021), p. 168. [10.1186/s12985-021-01638-5]
Abstract:
A growing number of emerging SARS-CoV-2 variants is being identified worldwide, potentially impacting the effectiveness of current vaccines. We report the data obtained in several Italian regions involved in the SARS-CoV-2 variant monitoring from the beginning of the epidemic and spanning the period... from October 2020 to March 2021.
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Quantification of the HIV-1 total reservoir in the peripheral blood of naïve and treated patients by a standardised method derived from a commercial HIV-1 RNA quantification assay.
Anno: 2021
Rivista: CLINICAL CHEMISTRY AND LABORATORY MEDICINE
Autori/autrici: Di Sante, L.; Costantini, A.; Caucci, S.; Corsi, A.; Brescini, L.; Menzo, S.; Bagnarelli, P.
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/283986
Citazioni:
Quantification of the HIV-1 total reservoir in the peripheral blood of naïve and treated patients by a standardised method derived from a commercial HIV-1 RNA quantification assay / Di Sante, L., Costantini, A., Caucci, S., Corsi, A., Brescini, L., Menzo, S., Bagnarelli, P.. - In: CLINICAL CHEMISTRY AND LABORATORY MEDICINE. - ISSN 1434-6621. - STAMPA. - 59:3(2021), pp. 609-617. [10.1515/cclm-2020-0142]
Abstract:
HIV-1 DNA can persist in host cells, establishing a latent reservoir. This study was aimed to develop an extraction and amplification protocol for HIV-1 DNA quantification by modifying a quantitative commercial assay. HIV-1 DNA was extracted on an Abbott m2000sp instrument, using an open-mode protoc...ol. Two calibrators, spiked with a plasmid containing HIV-1 genome (103 and 105 cps/mL), were extracted and amplified to generate a master calibration curve. Precision, accuracy, linear dynamic range, limit of quantification (LOQ) and limit of detection (LOD) were determined. A cohort of patients, naïve or chronically infected, was analysed. Calibration curve was obtained from 42 replicates of standards (stds); precision was calculated (coefficients of variability [CVs] below 10%); accuracy was higher than 90%. Linearity covered the entire range tested (10-104 copies per reaction), and LOD (95%) was 12 copies per reaction. HIV-1 DNA was significantly higher (p < 0.0001) in drug-naïve (62) than in chronically treated patients (50), and proviral loads correlated with lymphocytes (p = 0.0002) and CD4+ (p < 0.0001) counts only in naïve patients. Both groups displayed a significant inverse correlation between CD4+ nadir and proviral loads. A significant correlation (p < 0.0001) between viraemia and HIV-1 reservoir was disclosed. No significant difference was obtained from the comparison between proviral loads on whole blood and peripheral blood mononuclear cells (PBMCs) from the same patient. The novelty of our approach relies on the selection of appropriate reference standard extracted and amplified as clinical specimens avoiding any underestimation of the reservoir. Results confirm HIV-1 DNA as a marker of disease progression, supporting the relationship between the width of latent reservoir and the immunological status of the patient.
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Geographical and temporal distribution of SARS-CoV-2 clades in the WHO European Region, January to June 2020
Anno: 2020
Rivista: EUROSURVEILLANCE
Autori/autrici: E., Alm; E. K., Broberg; T., Connor; E. B., Hodcroft; A. B., Komissarov; S., Maurer-Stroh; A., Melidou; R. A., Neher; A., O'Toole; Niko Beerenwinkel, Pereyaslov D.; Posada-Céspedes, Susana; Philipp Jablonski, Kim; Falé Ferreira, Pedro; Topolsky, Ivan; Avšič-Županc, Tatjana; Korva, Miša; Poljak, Mario; Zakotnik, Samo; Mark Zorec, Tomaž; Bragstad, Karoline; Hungnes, Olav; Stene-Johansen, Kathrine; Reusken, Chantal; Meijer, Adam; Vennema, Harry; Ruiz-Roldán, Lidia; Alma Bracho, María; García-González, Neris; Chiner-Oms, Álvaro; Cancino-Muñoz, Irving; Comas, Iñaki; A Goig, Galo; Torres-Puente, Manuela; G López, Mariana; Martínez-Priego, Llúcia; D'Auria, Giuseppe; Ferrús-Abad, Loreto; de Marco, Griselda; Galan-Vendrell, Inmaculada; Carbó-Ramirez, Sandra; Ruíz-Hueso, Paula; Coscollá, Mireia; Polackova, Katerina; Kramna, Lenka; Cinek, Ondrej; Richter, Jan; Krashias, George; Tryfonos, Christina; Bashiardes, Stavros; Koptides, Dana; Christodoulou, Christina; Bartolini, Barbara; Em Gruber, Cesare; Di Caro, Antonino; Castilletti, Concetta; Stefani, Fabrizio; Giordana Rimoldi, Sara; Romeri, Francesca; Salerno, Franco; Polesello, Stefano; Nagy, Alexander; Jirincova, Helena; Vecerova, Jaromira; Novakova, Ludmila; Cordey, Samuel; Murtskhvaladze, Marine; Kotaria, Nato; Schär, Tobias; Beisel, Christian; Vugrek, Oliver; Rokić, Filip; Trgovec-Greif, Lovro; Jurak, Igor; Rukavina, Tomislav; Sučić, Neven; Schønning, Kristian; M Karst, Søren; H Kirkegaard, Rasmus; Y Michaelsen, Thomas; Aa Sørensen, Emil; Knutson, Simon; Brandt, Jakob; Le-Quy, Vang; Sørensen, Trine; Petersen, Celine; Schou Pedersen, Martin; Løkkegaard Larsen, Sanne; Nielsine Skov, Marianne; Rasmussen, Morten; Fonager, Jannik; Fomsgaard, Anders; Amirovich Maksyutov, Rinat; Vasil'Evna Gavrilova, Elena; Victorovich Pyankov, Oleg; Alexandrovich Bodnev, Sergey; Vladimirovna Tregubchak, Tatyana; Nikolayevich Shvalov, Alexander; Victorovich Antonets, Denis; Cristina Resende, Paola; Goya, Stephanie; Perrin, Amandine; Tc Lee, Raphael; Yadahalli, Shilpa; X Han, Alvin; A Russell, Colin; Schmutz, Stefan; Zaheri, Maryam; Kufner, Verena; Huber, Michael; Trkola, Alexandra; Antwerpen, Markus; C Walter, Mathias; van der Werf, Sylvie; Gambaro, Fabiana; Behillil, Sylvie; Enouf, Vincent; Donati, Flora; Ustinova, Monta; Rovite, Vita; Klovins, Janis; Savicka, Oksana; K Wienecke-Baldacchino, Anke; Ragimbeau, Catherine; Fournier, Guillaume; Mossong, Joël; W Aberle, Stephan; Haukland, Mattias; Enkirch, Theresa; Advani, Abdolreza; Lind Karlberg, Maria; Karlsson Lindsjö, Oskar; Broddesson, Sandra; Sláviková, Monika; Ličková, Martina; Klempa, Boris; Staroňová, Edita; Tichá, Elena; Szemes, Tomáš; Rusňáková, Diana; Stadler, Tanja; Quer, Josep; Anton, Andres; Andres, Cristina; Piñana, Maria; Garcia-Cehic, Damir; Pumarola, Tomas; Izopet, Jacques; Gioula, Georgia; Exindari, Maria; Papa, Anna; Chatzidimitriou, Dimitrios; Metallidis, Symeon; Pappa, Stella; Macek Jr, Milan; Geryk, Jan; Brož, Petr; Briksí, Aleš; Hubáček, Petr; Dřevínek, Pavel; Zajac, Miroslav; Kvapil, Petr; Holub, Michal; Kvapilová, Kateřina; Novotný, Adam; Kašný, Martin; Klempt, Petr; Vapalahti, Olli; Smura, Teemu; Sironen, Tarja; Selhorst, Philippe; Anthony, Colin; Ariën, Kevin; Simon-Loriere, Etienne; Rabalski, Lukasz; Bienkowska-Szewczyk, Krystyna; Borges, Vítor; Isidro, Joana; Paulo Gomes, João; Guiomar, Raquel; Pechirra, Pedro; Costa, Inês; Duarte, Sílvia; Vieira, Luís; Pyrc, Krzysztof; S Zuckerman, Neta; Turdikulova, Shahlo; Abdullaev, Alisher; Dalimova, Dilbar; Abdurakhimov, Abror; Tagliabracci, Adriano; Alessandrini, Federica; Melchionda, Filomena; Onofri, Valerio; Turchi, Chiara; Bagnarelli, Patrizia; Menzo, Stefano; Caucci, Sara; DI SANTE, Laura; Popa, Alexandra; Genger, Jakob-Wendelin; Agerer, Benedikt; Lercher, Alexander; Endler, Lukas; Smyth, Mark; Penz, Thomas; Schuster, Michael; Senekowitsch, Martin; Laine, Jan; Bock, Christoph; Bergthaler, Andreas; Shevtsov, Alexandr; Kalendar, Ruslan; Ramanculov, Yerlan; Graf, Alexander; Muenchhoff, Maximilian; T Keppler, Oliver; Krebs, Stefan; Blum, Helmut; Marcello, Alessandro; Licastro, Danilo; D'Agaro, Pierlanfranco; Laubscher, Florian; Vidanovic, Dejan; Tesovic, Bojana; Volkening, Jeremy; Clementi, Nicola; Mancini, Nicasio; Rupnik, Maja; Mahnic, Aleksander; Walker, Andreas; Houwaart, Torsten; Wienemann, Tobias; Kohns Vasconcelos, Malte; Strelow, Daniel; Ole Jensen, Björn-Erik; Senff, Tina; Hülse, Lisanna; Adams, Ortwin; Andree, Marcel; Hauka, Sandra; Feldt, Torsten; Keitel, Verena; Kindgen-Milles, Detlef; Timm, Jörg; Pfeffer, Klaus; T Dilthey, Alexander; Moore, Catherine; Ozdarendeli, Aykut; Terkis Islam Pavel, Shaikh; Yetiskin, Hazel; Aydin, Gunsu; Holyavkin, Can; Ali Uygut, Muhammet; Cevik, Ceren; Shchetinin, Alexey; Gushchin, Vladimir; Dinler-Doganay, Gizem; Doganay, Levent; Kizilboga-Akgun, Tugba; Karacan, Ilker; Pancer, Katarzyna; Maes, Piet; Martí-Carreras, Joan; Wawina-Bokalanga, Tony; Vanmechelen, Bert; Thürmer, Andrea; Wedde, Marianne; Dürrwald, Ralf; Von Kleist, Max; Drechsel, Oliver; Wolff, Thorsten; Fuchs, Stephan; Kmiecinski, Rene; Michel, Janine; Nitsche, Andreas; Casas, Inmaculada; Iglesias Caballero, María; Zaballos, Ángel; Jiménez, Pilar; Jiménez, Mercedes; Monzón Fernández, Sara; Varona Fernández, Sarai; Cuesta De La Plaza, Isabel; Fadeev, Artem; Ivanova, Anna; Sergeeva, Mariia; Stefanelli, Paola; Estee Torok, M; Hall, Grant; da Silva Filipe, Ana; Turtle, Lance; Afifi, Safiah; Mccluggage, Kathryn; Beer, Robert; Ledesma, Juan; Maksimovic, Joshua; Spellman, Karla; L Hamilton, William; Marchbank, Angela; Alexander Southgate, Joel; Underwood, Anthony; Taylor, Ben; Yeats, Corin; Abudahab, Khalil; R Gemmell, Matthew; Eccles, Richard; Lucaci, Anita; Abigail Nelson, Charlotte; Rainbow, Lucille; Whitehead, Mark; Gregory, Richard; Haldenby, Sam; Paterson, Steve; A Hughes, Margaret; D Curran, Martin; Baker, David; Tucker, Rachel; R Green, Luke; Feltwell, Theresa; D Halstead, Fenella; Wyles, Matthew; S Jahun, Aminu; Y Ahmad, Shazaad S; Georgana, Iliana; Goodfellow, Ian; Yakovleva, Anna; W Meredith, Luke; Gavriil, Artemis; Raza Awan, Ali; Fisher, Chloe; Edgeworth, Jonathan; Lynch, Jessica; Moore, Nathan; Williams, Rebecca; P Kidd, Stephen; Cortes, Nicholas; Brunker, Kirstyn; T Mccrone, John; Quick, Joshua; Duckworth, Nichola; Walsh, Sarah; Sloan, Tim; Ludden, Catherine; P George, Ryan; Eltringham, Gary; R Brown, Julianne; Aranday-Cortes, Elihu; G Shepherd, James; Hughes, Joseph; K Li, Kathy; C Williams, Thomas; Johnson, Natasha; Jesudason, Natasha; Mair, Daniel; Thomson, Emma; Shah, Rajiv; A Parr, Yasmin; Carmichael, Stephen; L Robertson, David; Nomikou, Kyriaki; Broos, Alice; Niebel, Marc; Smollett, Katherine; Tong, Lily; Miah, Shahjahan; Wittner, Anita; Phillips, Nicole; Payne, Brendan; Dewar, Rebecca; Holmes, Alison; Bolt, Frances; R Price, James; Mookerjee, Siddharth; K Sethi, Dheeraj; Potter, Will; Stanley, Rachael; Prakash, Reenesh; Dervisevic, Samir; Clive Graham, Jonathan; Nelson, Andrew; Smith, Darren; R Young, Gregory; Chyin Yew, Wen; A Todd, John; Trebes, Amy; Andersson, Monique; Bull, Matthew; Watkins, Joanne; Birchley, Alec; Gatica-Wilcox, Bree; Gilbert, Lauren; Kumžiene-Summerhayes, Sara; Rey, Sara; Chauhan, Anoop; Butcher, Ethan; Bicknell, Kelly; Elliott, Scott; Glaysher, Sharon; Lackenby, Angie; Bibby, David; Platt, Steven; Mohamed, Hodan; William Machin, Nicholas; Lutamyo Mbisa, Jean; Evans, Jonathan; Perry, Malorie; Pacchiarini, Nicole; Corden, Sally; Geraint Adams, Alexander; Gaskin, Amy; Coombs, Jason; John Graham, Lee; Cottrell, Simon; Morgan, Mari; Gifford, Laura; Kolyva, Anastasia; John Rudder, Steven; J Trotter, Alexander; E Mather, Alison; Aydin, Alp; J Page, Andrew; L Kay, Gemma; de Oliveira Martins, Leonardo; Yasir, Muhammad; Alikhan, Nabil-Fareed; M Thomson, Nicholas; Gilroy, Rachel; A Kingsley, Robert; O'Grady, Justin; Victoria Gutierrez, Ana; Diaz, Maria; Le Viet, Thanh; P Tedim, Ana; M Adriaenssens, Evelien; Patrick Mcclure, C; Moore, Christopher; Sang, Fei; Clark, Gemma; C Howson-Wells, Hannah; Debebe, Johnny; Ball, Jonathan; Chappell, Joseph; Khakh, Manjinder; Carlile, Matthew; Loose, Matthew; M Lister, Michelle; Holmes, Nadine; Tsoleridis, Theocharis; M Fleming, Vicki; Wright, Victoria; Smith, Wendy; D Gallagher, Michael; Parker, Matthew; G Partridge, David; Evans, Cariad; Baker, Paul; Essex, Sarah; Liggett, Steven; J Keeley, Alexander; Bashton, Matthew; Rooke, Stefan; Dervisevic, Samir; Jane Meader, Emma; Enrique Balcazar Lopez, Carlos; Angyal, Adrienn; Kristiansen, Mark; J Tutill, Helena; Findlay, Jacqueline; Mestek-Boukhibar, Lamia; Forrest, Leysa; Dyal, Patricia; J Williams, Rachel; Panchbhaya, Yasmin; A Williams, Charlotte; Roy, Sunando; Pandey, Sarojini; Stockton, Jo; J Loman, Nicholas; Poplawski, Radoslaw; Nicholls, Samuel; M Rowe, W P; Khokhar, Fahad; Lars Pinckert, Malte; Hosmillo, Myra; Chaudhry, Yasmin; G Caller, Laura; K Davidson, Rose; Griffith, Luke; Rambaut, Andrew; Jackson, Ben; Colquhoun, Rachel; Hill, Verity; Nichols, Jenna; Asamaphan, Patawee; Darby, Alistair; A Jackson, Kathryn; Iturriza-Gomara, Miren; Edith Vamos, Ecaterina; Green, Angie; Aanensen, David; Bonsall, David; Buck, David; Macintyre-Cockett, George; de Cesare, Mariateresa; Pybus, Oliver; Golubchik, Tanya; Scarlett, Garry; F Loveson, Katie; C Robson, Samuel; Beckett, Angela; Lindsey, Benjamin; C Groves, Danielle; J Parsons, Paul; P Mchugh, Martin; Daniel Barnes, James; F Manso, Carmen; Grammatopoulos, Dimitris; Elisabeth Menger, Katja; Harrison, Ewan; Gunson, Rory; J Peacock, Sharon; Gonzalez, Gabriel; Carr, Michael; Mihaela, Lazar; Popovici, Odette; Brytting, Mia; Bresner, Catherine; Fuller, William; Workman, Trudy; F Mentis, Andreas; Kossyvakis, Athanasios; Karamitros, Timokratis; Pogka, Vasiliki; Kalliaropoulos, Antonios; Horefti, Elina; Kontou, Aspasia; Martinez-Gonzalez, Beatriz; Labropoulou, Voula; Voulgari-Kokota, Androniki; Evangelidou, Maria; Bizta, Panagiota; Belimezi, Maria; Lambrechts, Laurens; Z Doymaz, Mehmet; Kalkan Yazici, Merve; S Cetin, Nesibe; Karaaslan, Elif; Kallio-Kokko, Hannimari; Virtanen, Jenni; Suvanto, Maija; Truong Nguyen, Phuoc; Ellonen, Pekka; Hannula, Sari; Kangas, Harri; B Sreenu, Vattipally; Burián, Katalin; Terhes, Gabriella; Gombos, Katalin; Gyenesei, Attila; Urbán, Péter; Herczeg, Róbert; Jakab, Ferenc; Kemenesi, Gábor; Endre Tóth, Gábor; Somogyi, Balázs; Zana, Brigitta; Zeghbib, Safia; Kuczmog, Anett; Földes, Fanni; Lanszki, Zsófia; Madai, Mónika; Papp, Henrietta; Nagy, Ágnes; István Pereszlényi, Csaba; Csaba Babinszky, Gergely; Dudás, Gábor; Csoma, Eszter; N Abou Tayoun, Ahmad; A Alsheikh-Ali, Alawi; Loney, Tom; Nowotny, Norbert; Abdul-Wahab, Osama; Gonzalez-Candelas, Fernando; H Andersen, Martin; Sarah Taylor, And
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/283651
Citazioni:
Geographical and temporal distribution of SARS-CoV-2 clades in the WHO European Region, January to June 2020 / E., A., E. K., B., T., C., E. B., H., A. B., K., S., M., A., M., R. A., N., A., O., Niko Beerenwinkel, P.D., Posada-Céspedes, S., Philipp Jablonski, K., Falé Ferreira, P., Topolsky, I., Avšič-Županc, T., Korva, M., Poljak, M., Zakotnik, S., Mark Zorec, T., Bragstad, K., et al.. - In: EUROSURVEILLANCE. - ISSN 1560-7917. - 25:32(2020). [10.2807/1560-7917.ES.2020.25.32.2001410]
Abstract:
We show the distribution of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) genetic clades over time and between countries and outline potential genomic surveillance objectives. We applied three genomic nomenclature systems to all sequence data from the World Health Organization Europea...n Region available until 10 July 2020. We highlight the importance of real-time sequencing and data dissemination in a pandemic situation, compare the nomenclatures and lay a foundation for future European genomic surveillance of SARS-CoV-2.
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Molecular tracing of SARS-CoV-2 in Italy in the first three months of the epidemic
Anno: 2020
Rivista: VIRUSES
Autori/autrici: Lai, A.; Bergna, A.; Caucci, S.; Clementi, N.; Vicenti, I.; Dragoni, F.; Cattelan, A. M.; Menzo, S.; Pan, A.; Callegaro, A.; Tagliabracci, A.; Caruso, A.; Caccuri, F.; Ronchiadin, S.; Balotta, C.; Zazzi, M.; Vaccher, E.; Clementi, M.; Galli, M.; Zehender, G.; Alessandrini, F; Andreoni, M; Antonelli, G; Babudieri, S; Bagnarelli, P; Bonora, S; Bruzzone, B; Brindicci, G; Carrer, P; Ceccherini, F; Codeluppi, M; Coluccello, A; Coppola, N; Cusi, Mg; C Della Ventura, ; DI SANTE, Laura; S Di Giambenedetto, ; G Di Perri, ; Fiore, V; Fiorentini, S; Francisci, D; Gandolfo, C; Gervasoni, C; Ghisetti, V; Greco, R; Guarona, G; Iannetta, M; L Li Puma, ; Malagnino, V.; Mancuso, G; Mastroianni, C; Menozzi, I; Milano, E; Miola, A; Morganti, M; Monno, L; Noberasco, G; Nunnari, G; Onofri, V; Orsi, A; Pierfranceschi, Mg; Pongolini, S; Ripamonti, D; Rubino, S; Ruggerone, L; Russignaga, D; Sagnelli, C; Sanguinetti, M; Sarmati, L; Sasset, L; Scaltriti, E; Schiavo, R; Schioppa, M; Testa, S; Turchi, C; Turriziani, O; E Venanzi Rollo, ; Zanussi, S; Zoncada, A
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/283713
Citazioni:
Molecular tracing of SARS-CoV-2 in Italy in the first three months of the epidemic / Lai, A., Bergna, A., Caucci, S., Clementi, N., Vicenti, I., Dragoni, F., Cattelan, A.M., Menzo, S., Pan, A., Callegaro, A., Tagliabracci, A., Caruso, A., Caccuri, F., Ronchiadin, S., Balotta, C., Zazzi, M., Vaccher, E., Clementi, M., Galli, M., Zehender, G., et al.. - In: VIRUSES. - ISSN 1999-4915. - 12:8(2020). [10.3390/v12080798]
Abstract:
The aim of this study is the characterization and genomic tracing by phylogenetic analyses of 59 new SARS-CoV-2 Italian isolates obtained from patients attending clinical centres in North and Central Italy until the end of April 2020. All but one of the newly-characterized genomes belonged to the li...neage B.1, the most frequently identified in European countries, including Italy. Only a single sequence was found to belong to lineage B. A mean of 6 nucleotide substitutions per viral genome was observed, without significant differences between synonymous and non-synonymous mutations, indicating genetic drift as a major source for virus evolution. tMRCA estimation confirmed the probable origin of the epidemic between the end of January and the beginning of February with a rapid increase in the number of infections between the end of February and mid-March. Since early February, an effective reproduction number (Re) greater than 1 was estimated, which then increased reaching the peak of 2.3 in early March, confirming the circulation of the virus before the first COVID-19 cases were documented. Continuous use of state-of-the-art methods for molecular surveillance is warranted to trace virus circulation and evolution and inform effective prevention and containment of future SARS-CoV-2 outbreaks.
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Trend of clinical vancomycin-resistant enterococci isolated in a regional Italian hospital from 2001 to 2018
Anno: 2020
Rivista: BRAZILIAN JOURNAL OF MICROBIOLOGY
Autori/autrici: Fioriti, S.; Simoni, S.; Caucci, S.; Morroni, G.; Ponzio, E.; Coccitto, S. N.; Brescini, L.; Cirioni, O.; Menzo, S.; Biavasco, F.; Giovanetti, E.; Brenciani, A.; Vignaroli, C.
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/283629
Citazioni:
Trend of clinical vancomycin-resistant enterococci isolated in a regional Italian hospital from 2001 to 2018 / Fioriti, S., Simoni, S., Caucci, S., Morroni, G., Ponzio, E., Coccitto, S.N., Brescini, L., Cirioni, O., Menzo, S., Biavasco, F., Giovanetti, E., Brenciani, A., Vignaroli, C.. - In: BRAZILIAN JOURNAL OF MICROBIOLOGY. - ISSN 1517-8382. - (2020). [10.1007/s42770-020-00360-8]
Abstract:
A retrospective study of the epidemiology of vancomycin-resistant enterococci (VRE) in a regional hospital of central Italy in 2001–2018 demonstrated an increased VRE prevalence since 2016. A total of 113 VRE isolates, 89 E. faecium (VREfm) and 24 E. faecalis (VREfs), were collected in the stu...dy period. All strains showed high-level resistance to vancomycin; 107 also showed teicoplanin resistance. Altogether, 84 VREfm and 20 VREfs carried vanA, whereas 5 VREfm and 1 VREfs carried vanB. MLST analysis documented that 89 VREfm isolates mainly belonged to ST78, ST80, and ST117. Most strains were isolated from 2001 to 2007, ST78 being the predominant clone. VREfm re-emerged in 2016 with a prevalence of the ST80 lineage. Most VREfs were isolated from 2001 to 2006; although they belonged to 7 different STs, there was a prevalence of ST88 and ST6. Notably, ST88 was sporadically recovered throughout the study period. The increasing rate of VREfm isolation from 2016 to 2018 may be related to the influx of new successful clones and to the renewed and widespread use of vancomycin. Improved infection control measures in hospital wards should be adopted to limit the spread of new epidemic VRE strains.
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Evaluation of the Ion AmpliSeq SARS-CoV-2 Research Panel by Massive Parallel Sequencing
Anno: 2020
Rivista: GENES
Autori/autrici: Alessandrini, F.; Caucci, S.; Onofri, V.; Melchionda, F.; Tagliabracci, A.; Bagnarelli, P.; Di Sante, L.; Turchi, C.; Menzo, S.
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/283652
Citazioni:
Evaluation of the Ion AmpliSeq SARS-CoV-2 Research Panel by Massive Parallel Sequencing / Alessandrini, F., Caucci, S., Onofri, V., Melchionda, F., Tagliabracci, A., Bagnarelli, P., Di Sante, L., Turchi, C., Menzo, S.. - In: GENES. - ISSN 2073-4425. - 11:8(2020), p. 929. [10.3390/genes11080929]
Abstract:
Deep knowledge of the genetic features of SARS-CoV-2 is essential to track the ongoing pandemic through different geographical areas and to design and develop early diagnostic procedures, therapeutic strategies, public health interventions, and vaccines. We describe protocols and first results of th...e Ion AmpliSeq™ SARS-CoV-2 Research Panel by a massively parallel sequencing (MPS) assay. The panel allows for targeted sequencing by overlapping amplicons, thereby providing specific, accurate, and high throughput analysis. A modified reverse transcription reaction, which consists of the use of a SARS-CoV-2 specific primers pool from the Ion AmpliSeq SARS-CoV-2 Research Panel, was assessed in order to promote viral RNA specific reverse transcription. The aim of this study was to evaluate the effectiveness of the Ion AmpliSeq™ SARS-CoV-2 Research Panel in sequencing the entire viral genome in different samples. SARS-CoV-2 sequence data were obtained from ten viral isolates and one nasopharyngeal swab from different patients. The ten isolate samples amplified with 12 PCR cycles displayed high mean depth values compared to those of the two isolates amplified with 20 PCR cycles. High mean depth values were also obtained for the nasopharyngeal swab processed by use of a target-specific reverse transcription. The relative depth of coverage (rDoC) analysis showed that when 12 PCR cycles were used, all target regions were amplified with high sequencing coverage, while in libraries amplified at 20 cycles, a poor uniformity of amplification, with absent or low coverage of many target regions, was observed. Our results show that the Ion AmpliSeq SARS-CoV-2 Research Panel can achieve rapid and high throughput SARS-CoV-2 whole genome sequencing from 10 ng of DNA-free viral RNA from isolates and from 1 ng of DNA-free viral RNA from a nasopharyngeal swab using 12 PCR cycles for library amplification. The modified RT-PCR protocol yielded superior results on the nasopharyngeal swab compared to the reverse transcription reaction set up according to the manufacturer's instructions.
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First Detection of SARS-CoV-2 by Real-Time Reverse Transcriptase-Polymerase Chain Reaction Assay in Pleural Fluid
Anno: 2020
Rivista: CHEST
Autori/autrici: Mei, Federico; Bonifazi, Martina; Menzo, Stefano; Di Marco Berardino, Alessandro; Sediari, Michele; Paolini, Luca; Re, Antonina; Gonnelli, Francesca; Duranti, Claudia; Grilli, Martina; Spurio Vennarucci, Giacomo; Agnese Latini, Maria; Zuccatosta, Lina; Gasparini, Stefano
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/286389
Citazioni:
First Detection of SARS-CoV-2 by Real-Time Reverse Transcriptase-Polymerase Chain Reaction Assay in Pleural Fluid / Mei, F., Bonifazi, M., Menzo, S., Di Marco Berardino, A., Sediari, M., Paolini, L., Re, A., Gonnelli, F., Duranti, C., Grilli, M., Spurio Vennarucci, G., Agnese Latini, M., Zuccatosta, L., Gasparini, S.. - In: CHEST. - ISSN 1931-3543. - ELETTRONICO. - 158:4(2020), pp. 143-146. [10.1016/j.chest.2020.05.583]
Abstract:
Coronavirus disease 2019 (COVID-19) is a pandemic infection due to the spread of a novel coronavirus (severe acute respiratory syndrome coronavirus 2), resulting in a wide range of clinical features, from asymptomatic carriers to ARDS. The gold standard for diagnosis is nucleic acid detection by rea...l-time reverse transcriptase-polymerase chain reaction in nasopharyngeal swabs. However, due to limitations in this technique’s sensitivity, thoracic imaging plays a crucial, complementary role in diagnostic evaluation and also allows for detection of atypical findings and potential alternative targets for sampling (eg, pleural effusion). Although less common, pleural involvement has been described in a minority of patients. This report describes the first case of reverse transcriptase-polymerase chain reaction detection of severe acute respiratory syndrome coronavirus 2 in pleural fluid obtained by means of ultrasoundguided thoracentesis, and its main characteristics are detailed. Pleural effusion is not a common finding in COVID-19 infection, but a prompt recognition of this potential localization may be useful to optimize diagnostic evaluation as well as the management of these patients.
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Antimicrobial Resistance: A Challenge for the Future
Anno: 2020
Autori/autrici: Varaldo, Pietro Emanuele; Facinelli, Bruna; Bagnarelli, Patrizia; Menzo, Stefano; Mingoia, Marina; Brenciani, Andrea; Giacometti, Andrea; Barchiesi, Francesco; Brescini, Lucia; Cirioni, Oscar; Scalise, Giorgio; Barbadoro, Pamela; Di Stanislao, Francesco; Prospero, Emilia; D'Errico, Marcello Mario
Lingua: ENG
Classificazione IRIS: 2.1 Contributo in volume (Capitolo o Saggio)
Scheda completa IRIS: https://iris.univpm.it/handle/11566/351592
Citazioni:
Antimicrobial Resistance: A Challenge for the Future / Varaldo, P.E., Facinelli, B., Bagnarelli, P., Menzo, S., Mingoia, M., Brenciani, A., Giacometti, A., Barchiesi, F., Brescini, L., Cirioni, O., Scalise, G., Barbadoro, P., Di Stanislao, F., Prospero, E., D'Errico, M.M.. - STAMPA. - (2020), pp. 13-29. [10.1007/978-3-030-33832-9_2]
Abstract:
The global emergence of antibiotic-resistance, together with the lack of/reduced development of new antibiotic molecules, currently represents a serious public health problem as it can mean the return to a pre-antibiotic era in which infections caused by multiple-resistant pathogens are intractable.... Since the beginnings, the interest of the Institutes of Microbiology, Hygiene and Public Health, and Infectious Diseases was focused on antibiotic resistance: from molecular mechanisms, through epidemiology and clinical issues, to prevention. Future perspectives include the search of new strategies and/or new compounds for prevention and control of difficult-to-treat pathogens in a multidisciplinary approach.
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Local Epidemics Gone Viral: Evolution and Diffusion of the Italian HIV-1 Recombinant Form CRF60_BC
Anno: 2019
Rivista: FRONTIERS IN MICROBIOLOGY
Autori/autrici: Lai, Alessia; Simonetti, Francesco Roberto; Brindicci, Gaetano; Bergna, Annalisa; Di Giambenedetto, Simona; Sterrantino, Gaetana; Mussini, Cristina; Menzo, Stefano; Bagnarelli, Patrizia; Zazzi, Maurizio; Angarano, Gioacchino; Galli, Massimo; Monno, Laura; Balotta, Claudia
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/266417
Citazioni:
Local Epidemics Gone Viral: Evolution and Diffusion of the Italian HIV-1 Recombinant Form CRF60_BC / Lai, A., Simonetti, F.R., Brindicci, G., Bergna, A., Di Giambenedetto, S., Sterrantino, G., Mussini, C., Menzo, S., Bagnarelli, P., Zazzi, M., Angarano, G., Galli, M., Monno, L., Balotta, C.. - In: FRONTIERS IN MICROBIOLOGY. - ISSN 1664-302X. - 10:(2019), p. 769. [10.3389/fmicb.2019.00769]
Abstract:
The molecular epidemiology of HIV-1 in Italy is becoming increasingly complex, mainly due to the spread of non-B subtypes and the emergence of new recombinant forms. We previously characterized the outbreak of the first Italian circulating recombinant form (CRF60_BC), occurring among young MSM livin...g in Apulia between the years 2009 and 2011. Here we show a 5-year follow-up surveillance to trace the evolution of CRF60_BC and to investigate its further spread in Italy. We collected additional sequences and clinical data from patients harboring CRF60_BC, enrolled at the Infectious Diseases Clinic of the University of Bari. In addition to the 24 previously identified sequences, we retrieved 27 CRF60_BC sequences from patients residing in Apulia, whose epidemiological and clinical features did not differ from those of the initial outbreak, i.e., the Italian origin, young age at HIV diagnosis (median: 24 years; range: 18-37), MSM risk factor (23/25, 92%) and recent infection (from 2008 to 2017). Sequence analysis revealed a growing overall nucleotide diversity, with few nucleotide changes that were fixed over time. Twenty-seven additional sequences were detected across Italy, spanning multiple distant regions. Using a BLAST search, we also identified a CRF60_BC sequence isolated in United Kingdom in 2013. Three patients harbored a unique second generation recombinant form in which CRF60_BC was one of the parental strains. Our data show that CRF60_BC gained epidemic importance, spreading among young MSM in multiple Italian regions and increasing its population size in few years, as the number of sequences identified so far has triplicated since our first report. The observed further divergence of CRF60_BC is likely due to evolutionary bottlenecks and host adaptation during transmission chains. Of note, we detected three second-generation recombinants, further supporting a widespread circulation of CRF60_BC and the increasing complexity of the HIV-1 epidemic in Italy.
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Increase and diversity of carbapenemase-producing Escherichia coli isolates, Italy
Anno: 2019
Rivista: FUTURE MICROBIOLOGY
Autori/autrici: Simoni, S; Caucci, Sara; Brenciani, A; Morroni, G; Giovanetti, E; Menzo, S; Facinelli, B; Mingoia, M.
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/269541
Citazioni:
Increase and diversity of carbapenemase-producing Escherichia coli isolates, Italy / Simoni, S., Caucci, S., Brenciani, A., Morroni, G., Giovanetti, E., Menzo, S., Facinelli, B., Mingoia, M.. - In: FUTURE MICROBIOLOGY. - ISSN 1746-0913. - STAMPA. - 14:2(2019), pp. 1035-1042. [10.2217/fmb-2019-0069]
Abstract:
Aim: This study reports on a surveillance in an Italian hospital focused on carbapenemase-producing Escherichia coli (CP-Ec). Materials & methods: Eighteen isolates (nine from clinical specimens and nine from rectal swab) were characterized for antibiotic susceptibilities, typing features, main ...carbapenemase, extended-spectrum ß-lactamases (ESBLs) and other bla genes, and their transferability by conjugation and transformation. Results: An increase in CP-Ec isolates was observed during 3-year surveillance period. Compared with the clinical isolates, all belonging to one sequence type (ST), ST131, those from rectal swab were very heterogeneous and belonged to eight STs. Transfer data confirmed the role of conjugative plasmids in the spreading of carbapenemase genes. Conclusion: The prevalence of CP-Ec in Italy has risen, with a substantial increase over the last year.
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Locally acquired hepatitis E virus in Marche Italy: Clinical/laboratory features and outcome
Anno: 2019
Rivista: DIGESTIVE AND LIVER DISEASE
Autori/autrici: Tarantino, G.; Ortolani, A.; Marinelli, K.; Benedetti, A.; Marconi, G.; Calzolari, M.; Dalton, H. R.; Marzioni, M.; Schiada, L.; Fava, G.; Chiodera, A.; Amadio, G.; Fiorentini, A.; Riva, A.; Fraticelli, P.; Menzo, S.; Bagnarelli, P.
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/273128
Citazioni:
Locally acquired hepatitis E virus in Marche Italy: Clinical/laboratory features and outcome / Tarantino, G., Ortolani, A., Marinelli, K., Benedetti, A., Marconi, G., Calzolari, M., Dalton, H.R., Marzioni, M., Schiada, L., Fava, G., Chiodera, A., Amadio, G., Fiorentini, A., Riva, A., Fraticelli, P., Menzo, S., Bagnarelli, P.. - In: DIGESTIVE AND LIVER DISEASE. - ISSN 1590-8658. - STAMPA. - (2019). [10.1016/j.dld.2019.11.015]

Long-term follow-up of acute hepatitis B: New insights in its natural history and implications for antiviral treatment
Anno: 2018
Rivista: GENES
Autori/autrici: Menzo, Stefano; Minosse, Claudia; Vincenti, Donatella; Vincenzi, Laura; Iacomi, Fabio; Zaccaro, Paola; D’Offizi, Gianpiero; Capobianchi, Maria R.
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/266412
Citazioni:
Long-term follow-up of acute hepatitis B: New insights in its natural history and implications for antiviral treatment / Menzo, S., Minosse, C., Vincenti, D., Vincenzi, L., Iacomi, F., Zaccaro, P., D’Offizi, G., Capobianchi, M.R.. - In: GENES. - ISSN 2073-4425. - 9:6(2018), p. 293. [10.3390/genes9060293]

Evolutionary trends of resistance mutational patterns of HBV reverse transcriptase over years (2002–2012) of different treatment regimens: The legacy of lamivudine/adefovir combination treatment
Anno: 2017
Rivista: ANTIVIRAL RESEARCH
Autori/autrici: Vincenti, Donatella; Piselli, Pierluca; Solmone, Mariacarmela; D'Offizi, Gianpiero; Capobianchi, Maria R.; Menzo, Stefano
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/249672
Citazioni:
Evolutionary trends of resistance mutational patterns of HBV reverse transcriptase over years (2002–2012) of different treatment regimens: The legacy of lamivudine/adefovir combination treatment / Vincenti, D., Piselli, P., Solmone, M., D'Offizi, G., Capobianchi, M.R., Menzo, S.. - In: ANTIVIRAL RESEARCH. - ISSN 0166-3542. - 143:(2017), pp. 62-68. [10.1016/j.antiviral.2017.03.008]

A clone of linezolid-resistant Staphylococcus epidermidis bearing the G2576T mutation is endemic in an Italian hospital
Anno: 2016
Rivista: THE JOURNAL OF HOSPITAL INFECTION
Autori/autrici: Morroni, Gianluca; Brenciani, Andrea; Vincenzi, Chiara; Barocci, Simone; Tili, Emily; Manso, E; Mingoia, Marina; Menzo, Stefano; Varaldo, Pietro; Giovanetti, Eleonora
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/236693
Citazioni:
A clone of linezolid-resistant Staphylococcus epidermidis bearing the G2576T mutation is endemic in an Italian hospital / Morroni, G., Brenciani, A., Vincenzi, C., Barocci, S., Tili, E., Manso, E., Mingoia, M., Menzo, S., Varaldo, P., Giovanetti, E.. - In: THE JOURNAL OF HOSPITAL INFECTION. - ISSN 0195-6701. - ELETTRONICO. - 94:(2016), pp. 203-206. [10.1016/j.jhin.2016.07.012]

Simple and reliable method to quantify the hepatitis B viral load and replicative capacity in liver tissue and blood leukocytes
Anno: 2016
Rivista: HEPATITIS MONTHLY
Autori/autrici: Minosse, Claudia; Coen, Sabrina; Comandini, Ubaldo Visco; Lionetti, Raffaella; Montalbano, Marzia; Cerilli, Stefano; Vincenti, Donatella; Baiocchini, Andrea; Capobianchi, Maria R.; Menzo, Stefano
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/266418
Citazioni:
Simple and reliable method to quantify the hepatitis B viral load and replicative capacity in liver tissue and blood leukocytes / Minosse, C., Coen, S., Comandini, U.V., Lionetti, R., Montalbano, M., Cerilli, S., Vincenti, D., Baiocchini, A., Capobianchi, M.R., Menzo, S.. - In: HEPATITIS MONTHLY. - ISSN 1735-143X. - 16:10(2016), p. e28751. [10.5812/hepatmon.28751]

Hepatitis E in a region of Italy: An emerging autochthonous infection?
Anno: 2016
Rivista: DIGESTIVE AND LIVER DISEASE
Autori/autrici: Tarantino, Giuseppe; Bagnarelli, Patrizia; Marzioni, Marco; Marinelli, Katia; Surace, Giulia; Traini, Sara; Baroni, Gianluca Svegliati; Menzo, Stefano; Benedetti, Antonio; SVEGLIATI BARONI, Gianluca
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/241092
Citazioni:
Hepatitis E in a region of Italy: An emerging autochthonous infection? / Tarantino, G., Bagnarelli, P., Marzioni, M., Marinelli, K., Surace, G., Traini, S., Baroni, G.S., Menzo, S., Benedetti, A., SVEGLIATI BARONI, G.. - In: DIGESTIVE AND LIVER DISEASE. - ISSN 1590-8658. - 48:11(2016), pp. 1340-1345. [10.1016/j.dld.2016.06.035]

Prophylaxis of HCV reinfection and direct-acting antiviral agents during liver transplantation
Anno: 2015
Rivista: LIVER TRANSPLANTATION
Autori/autrici: Tavio, Marcello; Vivarelli, Marco; Menzo, Stefano; Gori, Andrea; Grossi, Paolo Antonio; Marigliano, Anna
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/231369
Citazioni:
Prophylaxis of HCV reinfection and direct-acting antiviral agents during liver transplantation / Tavio, M., Vivarelli, M., Menzo, S., Gori, A., Grossi, P.A., Marigliano, A.. - In: LIVER TRANSPLANTATION. - ISSN 1527-6465. - STAMPA. - 21:10(2015), pp. 1327-1329. [10.1002/lt.24204]

A statistical model based on serological parameters for predicting occult HBV infection: implications for organ/ blood donations
Anno: 2015
Rivista: NEW MICROBIOLOGICA
Autori/autrici: Coen, S.; Angeletti, C.; Piselli, P.; Tronchin, M.; Vincenti, D.; Capobianchi, M. R.; Galli, C.; Menzo, Stefano
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/222915
Citazioni:
A statistical model based on serological parameters for predicting occult HBV infection: implications for organ/ blood donations / Coen, S., Angeletti, C., Piselli, P., Tronchin, M., Vincenti, D., Capobianchi, M.R., Galli, C., Menzo, S.. - In: NEW MICROBIOLOGICA. - ISSN 1121-7138. - (2015).

A large epidemic of hepatitis B in Serbia: an integrated model for outbreak investigations in healthcare settings
Anno: 2014
Rivista: INFECTION CONTROL AND HOSPITAL EPIDEMIOLOGY
Autori/autrici: Lanini, S.; Ćosić, G.; Menzo, Stefano; Puro, V.; Đurić, P.; Garbuglia, A. R.; Milošević, V.; Karać, T.; Capobianchi, M. R.; Ippolito, G.
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/222914
Citazioni:
A large epidemic of hepatitis B in Serbia: an integrated model for outbreak investigations in healthcare settings / Lanini, S., Ćosić, G., Menzo, S., Puro, V., Đurić, P., Garbuglia, A.R., Milošević, V., Karać, T., Capobianchi, M.R., Ippolito, G.. - In: INFECTION CONTROL AND HOSPITAL EPIDEMIOLOGY. - ISSN 0899-823X. - 35:6(2014), pp. 732-735. [10.1086/676432]

Declining prevalence of HIV-1 drug resistance in antiretroviral treatment-exposed individuals in Western Europe.
Anno: 2013
Rivista: THE JOURNAL OF INFECTIOUS DISEASES
Autori/autrici: De Luca, A1; Dunn, D; Zazzi, M; Camacho, R; Torti, C; Fanti, I; Kaiser, R; Sönnerborg, A; Codoñer, Fm; Van Laethem, K; Vandamme, Am; Bansi, L; Ghisetti, V; van de Vijver, Da; Asboe, D; Prosperi, Mc; Di Giambenedetto, S; Menzo, Stefano
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/163740
Citazioni:
Declining prevalence of HIV-1 drug resistance in antiretroviral treatment-exposed individuals in Western Europe / De Luca, A.1., Dunn, D., Zazzi, M., Camacho, R., Torti, C., Fanti, I., Kaiser, R., Sönnerborg, A., Codoñer, F.m., Van Laethem, K., Vandamme, A.m., Bansi, L., Ghisetti, V., van de Vijver, D.a., Asboe, D., Prosperi, M.c., Di Giambenedetto, S., Menzo, S.. - In: THE JOURNAL OF INFECTIOUS DISEASES. - ISSN 0022-1899. - STAMPA. - (2013). [10.1093/infdis/jit017]

Slow response to entecavir treatment in treatment-naive HBV patients is conditioned by immune response rather than by the presence or selection of refractory variants
Anno: 2013
Rivista: ANTIVIRAL THERAPY
Autori/autrici: Mariacarmela, Solmone; Emanuela, Giombini; Donatella, Vincenti; Gabriella, Rozera; Angela, Testa; Alessandra, Moscetti; Manuela, Catalano; Isabella, Abbate; Maria R., Capobianchi; Menzo, Stefano
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/163739
Citazioni:
Slow response to entecavir treatment in treatment-naive HBV patients is conditioned by immune response rather than by the presence or selection of refractory variants / Mariacarmela, S., Emanuela, G., Donatella, V., Gabriella, R., Angela, T., Alessandra, M., Manuela, C., Isabella, A., Maria R., C., Menzo, S.. - In: ANTIVIRAL THERAPY. - ISSN 1359-6535. - 19:(2013), pp. 201-209. [10.3851/IMP2700]

42. Adenoviridae
Anno: 2012
Autori/autrici: Menzo, Stefano
Classificazione IRIS: 2.1 Contributo in volume (Capitolo o Saggio)
Scheda completa IRIS: https://iris.univpm.it/handle/11566/83481
Citazioni:
42. Adenoviridae / Menzo, S.. - (2012), pp. B97-B27.

Immune response, rather than sensitivity to entecavir, may determine the rate of viral decay upon treatment initiation in patients with chronic HBV infection
Anno: 2012
Rivista: HEPATOLOGY
Autori/autrici: Solmone, Mc; Giombini, E; Vincenti, D; Testa, A; Rozera, G; Abbate, I; Capobianchi, Mr; Menzo, Stefano
Lingua: ENG
Classificazione IRIS: 4.2 Abstract in Atti di convegno
Scheda completa IRIS: https://iris.univpm.it/handle/11566/83475
Citazioni:
Immune response, rather than sensitivity to entecavir, may determine the rate of viral decay upon treatment initiation in patients with chronic HBV infection / Solmone, M.c., Giombini, E., Vincenti, D., Testa, A., Rozera, G., Abbate, I., Capobianchi, M.r., Menzo, S.. - In: HEPATOLOGY. - ISSN 0270-9139. - STAMPA. - 56:(2012), p. 405A. (The Liver Meeting Boston Novembre 2012).

Comparison of HIV-1 recombinant phenotypic assays and virtual phenotype in the definition of coreceptor usage
Anno: 2011
Rivista: INFECTION
Autori/autrici: Monachetti, Alessia; Biagioni, G.; Ferreri, M.; Marinelli, K.; Menzo, Stefano; Bagnarelli, Patrizia
Lingua: ENG
Classificazione IRIS: 1.5 Abstract in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/80390
Citazioni:
Comparison of HIV-1 recombinant phenotypic assays and virtual phenotype in the definition of coreceptor usage / Monachetti, A., Biagioni, G., Ferreri, M., Marinelli, K., Menzo, S., Bagnarelli, P.. - In: INFECTION. - ISSN 0300-8126. - 39 Supplement 1:(2011), pp. S49-S50.

No pol mutation is associated independently with the lack of immune recovery in patients infected with HIV and failing antiretroviral therapy.
Anno: 2011
Rivista: JOURNAL OF MEDICAL VIROLOGY
Autori/autrici: Gianotti, N; Galli, L; Zazzi, M; Ghisetti, V; Bonora, S; Micheli, V; Meraviglia, P; Corsi, P; Bruzzone, B; Menzo, Stefano; Di Giambenedetto, S; De Luca, A; Filice, G; Penco, G; Castagna, A; Giacometti, Andrea
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/73913
Citazioni:
No pol mutation is associated independently with the lack of immune recovery in patients infected with HIV and failing antiretroviral therapy / Gianotti, N., Galli, L., Zazzi, M., Ghisetti, V., Bonora, S., Micheli, V., Meraviglia, P., Corsi, P., Bruzzone, B., Menzo, S., Di Giambenedetto, S., De Luca, A., Filice, G., Penco, G., Castagna, A., Giacometti, A.. - In: JOURNAL OF MEDICAL VIROLOGY. - ISSN 0146-6615. - 83:(2011). [10.1002/jmv.21989]

A prognostic model for estimating the time to virologic failure in HIV-1 infected patients undergoing a new combination antiretroviral therapy regimen.
Anno: 2011
Rivista: BMC MEDICAL INFORMATICS AND DECISION MAKING
Autori/autrici: Prosperi, M. C.; Di Giambenedetto, S.; Fanti, I.; Meini, G.; Bruzzone, B.; Callegaro, A.; Penco, G.; Bagnarelli, Patrizia; Micheli, V.; Paolini, E.; Di Biagio, A.; Ghisetti, V.; Di Pietro, M.; Zazzi, M.; De Luca, A.; Menzo, Stefano
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/64357
Citazioni:
A prognostic model for estimating the time to virologic failure in HIV-1 infected patients undergoing a new combination antiretroviral therapy regimen / Prosperi, M.C., Di Giambenedetto, S., Fanti, I., Meini, G., Bruzzone, B., Callegaro, A., Penco, G., Bagnarelli, P., Micheli, V., Paolini, E., Di Biagio, A., Ghisetti, V., Di Pietro, M., Zazzi, M., De Luca, A., Menzo, S.. - In: BMC MEDICAL INFORMATICS AND DECISION MAKING. - ISSN 1472-6947. - 14/2011:(2011), pp. 11-40. [10.1186/1472-6947-11-40]

Human case of autochthonous West Nile virus lineage 2 infection in Italy, September 2011.
Anno: 2011
Rivista: EUROSURVEILLANCE
Autori/autrici: Bagnarelli, Patrizia; Marinelli, K.; Trotta, D.; Monachetti, Alessia; Tavio, M.; Del Gobbo, R.; Capobianchi, M.; Menzo, Stefano; Nicoletti, L.; Magurano, F.; Varaldo, Pietro
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/64358
Citazioni:
Human case of autochthonous West Nile virus lineage 2 infection in Italy, September 2011 / Bagnarelli, P., Marinelli, K., Trotta, D., Monachetti, A., Tavio, M., Del Gobbo, R., Capobianchi, M., Menzo, S., Nicoletti, L., Magurano, F., Varaldo, P.. - In: EUROSURVEILLANCE. - ISSN 1560-7917. - 16(43)/2011:(2011), pp. 1-4.

Hemagglutinin 222 variants in pandemic (H1N1) 2009 virus.
Anno: 2011
Rivista: EMERGING INFECTIOUS DISEASES
Autori/autrici: Valli, Mb; Selleri, M; Meschi, S; Zaccaro, P; Vincenti, D; Lalle, E; Capobianchi, Mr; Menzo, Stefano
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/83422
Citazioni:
Hemagglutinin 222 variants in pandemic (H1N1) 2009 virus / Valli, M.b., Selleri, M., Meschi, S., Zaccaro, P., Vincenti, D., Lalle, E., Capobianchi, M.r., Menzo, S.. - In: EMERGING INFECTIOUS DISEASES. - ISSN 1080-6040. - 17:(2011). [10.3201/eid1706.100784]

Insulin-like growth factor 1 (IGF-1) expression correlates with clinical outcome in K-RAS wild type colorectal cancer patients treated with cetuximab-irinotecan.
Anno: 2010
Rivista: INTERNATIONAL JOURNAL OF CANCER
Autori/autrici: Scartozzi, M; Mandolesi, A; Giampieri, Riccardo; Pierantoni, C; Loupakis, F; Zaniboni, A; Galizia, E; Giustini, L; Rita, R; Bisonni, R; Berardi, Rossana; Biagetti, S; Menzo, Stefano; Falcone, A; Bearzi, I; Cascinu, S.
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/37514
Citazioni:
Insulin-like growth factor 1 (IGF-1) expression correlates with clinical outcome in K-RAS wild type colorectal cancer patients treated with cetuximab-irinotecan / Scartozzi, M., Mandolesi, A., Giampieri, R., Pierantoni, C., Loupakis, F., Zaniboni, A., Galizia, E., Giustini, L., Rita, R., Bisonni, R., Berardi, R., Biagetti, S., Menzo, S., Falcone, A., Bearzi, I., Cascinu, S.. - In: INTERNATIONAL JOURNAL OF CANCER. - ISSN 0020-7136. - (2010).

Insulin-like growth factor 1 expression correlates with clinical outcome in K-RAS wild type colorectal cancer patients treated with cetuximab and irinotecan
Anno: 2010
Rivista: INTERNATIONAL JOURNAL OF CANCER
Autori/autrici: Scartozzi, M.; Mandolesi, A.; Giampieri, Riccardo; Pierantoni, C.; Loupakis, F.; Zaniboni, A.; Galizia, E.; Giustini, L.; Silva, R. R.; Bisonni, R.; Berardi, Rossana; Biagetti, S.; Menzo, Stefano; Falcone, A.; Bearzi, I.; Cascinu, S.
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/56475
Citazioni:
Insulin-like growth factor 1 expression correlates with clinical outcome in K-RAS wild type colorectal cancer patients treated with cetuximab and irinotecan / Scartozzi, M., Mandolesi, A., Giampieri, R., Pierantoni, C., Loupakis, F., Zaniboni, A., Galizia, E., Giustini, L., Silva, R.R., Bisonni, R., Berardi, R., Biagetti, S., Menzo, S., Falcone, A., Bearzi, I., Cascinu, S.. - In: INTERNATIONAL JOURNAL OF CANCER. - ISSN 0020-7136. - 127:(2010), pp. 1941-1947. [10.1002/ijc.25193]

Absence of neuroinvasive disease in a liver transplant recipient who acquired West Nile virus (WNV) infection from the organ donor and who received WNV antibodies prophylactically.
Anno: 2010
Rivista: CLINICAL INFECTIOUS DISEASES
Autori/autrici: Morelli, Mc; Sambri, V; Grazi, Gl; Gaibani, P; Pierro, A; Cescon, M; Ercolani, G; Cavrini, F; Rossini, G; Capobianchi, Mr; Di Caro, A; Menzo, Stefano; Pagliaro, Pp; Ghinelli, F; Lazzarotto, T; Landini, Mp; Pinna, Ad
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/83420
Citazioni:
Absence of neuroinvasive disease in a liver transplant recipient who acquired West Nile virus (WNV) infection from the organ donor and who received WNV antibodies prophylactically / Morelli, M.c., Sambri, V., Grazi, G.l., Gaibani, P., Pierro, A., Cescon, M., Ercolani, G., Cavrini, F., Rossini, G., Capobianchi, M.r., Di Caro, A., Menzo, S., Pagliaro, P.p., Ghinelli, F., Lazzarotto, T., Landini, M.p., Pinna, A.d.. - In: CLINICAL INFECTIOUS DISEASES. - ISSN 1058-4838. - 51:(2010). [10.1086/655146]

Evolutionary pattern of pandemic influenza (H1N1) 2009 virus in the late phases of the 2009 pandemic.
Anno: 2010
Autori/autrici: Valli, Mb; Meschi, S; Selleri, M; Zaccaro, P; Ippolito, G; Capobianchi, Mr; Menzo, Stefano
Lingua: ENG
Classificazione IRIS: 2.1 Contributo in volume (Capitolo o Saggio)
Scheda completa IRIS: https://iris.univpm.it/handle/11566/83996
Citazioni:
Evolutionary pattern of pandemic influenza (H1N1) 2009 virus in the late phases of the 2009 pandemic / Valli, M.b., Meschi, S., Selleri, M., Zaccaro, P., Ippolito, G., Capobianchi, M.r., Menzo, S.. - (2010). [10.1371/currents.RRN1149]

Prone Positioning and Intravenous Zanamivir may Represent Effective Alternatives for Patients with Severe ARDS Virus A (H1N1) Related Pneumonia in Hospitals with no Access to ECMO.
Anno: 2010
Rivista: CRITICAL CARE RESEARCH AND PRACTICE
Autori/autrici: Gristina, G; Nardi, G; Orazi, D; Lauria, Fn; Valli, Mb; Lalle, E; Menzo, Stefano; Riccioni, L; Camporiondo, Mp
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/83421
Citazioni:
Prone Positioning and Intravenous Zanamivir may Represent Effective Alternatives for Patients with Severe ARDS Virus A (H1N1) Related Pneumonia in Hospitals with no Access to ECMO / Gristina, G., Nardi, G., Orazi, D., Lauria, F.n., Valli, M.b., Lalle, E., Menzo, S., Riccioni, L., Camporiondo, M.p.. - In: CRITICAL CARE RESEARCH AND PRACTICE. - ISSN 2090-1305. - 2010:(2010). [10.1155/2010/146456]

Evolution and predictors of HIV type-1 drug resistance in patients failing combination antiretroviral therapy in Italy.
Anno: 2009
Rivista: ANTIVIRAL THERAPY
Autori/autrici: Di Giambenedetto, S; Zazzi, M; Corsi, P; Gonnelli, A; Di Pietro, M; Giacometti, Andrea; Almi, P; Trezzi, M; Boeri, E; Gianotti, N; Menzo, Stefano; Del Gobbo, R; Francisci, D; Nerli, A; Galli, L; De Luca, A; Antiretroviral Resistance Cohort Analysis Study, Group
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/54037
Citazioni:
Evolution and predictors of HIV type-1 drug resistance in patients failing combination antiretroviral therapy in Italy / Di Giambenedetto, S., Zazzi, M., Corsi, P., Gonnelli, A., Di Pietro, M., Giacometti, A., Almi, P., Trezzi, M., Boeri, E., Gianotti, N., Menzo, S., Del Gobbo, R., Francisci, D., Nerli, A., Galli, L., De Luca, A., Antiretroviral Resistance Cohort Analysis Study, G.. - In: ANTIVIRAL THERAPY. - ISSN 1359-6535. - 14:(2009), pp. 359-369.

Low-abundance drug resistance mutations: extending the HIV paradigm to hepatitis B virus.
Anno: 2009
Rivista: THE JOURNAL OF INFECTIOUS DISEASES
Autori/autrici: Menzo, Stefano; Vincenti, D; Solmone, M; Prosperi, M; Bruselles, A; Abbate, I; Rozera, G; Capobianchi, Mr
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/83435
Citazioni:
Low-abundance drug resistance mutations: extending the HIV paradigm to hepatitis B virus / Menzo, S., Vincenti, D., Solmone, M., Prosperi, M., Bruselles, A., Abbate, I., Rozera, G., Capobianchi, M.r.. - In: THE JOURNAL OF INFECTIOUS DISEASES. - ISSN 0022-1899. - 200:(2009). [10.1086/647991]

Surgery and topic cidofovir for nasal squamous papillomatosis in HIV+ patient.
Anno: 2009
Rivista: EUROPEAN ARCHIVES OF OTO-RHINO-LARYNGOLOGY
Autori/autrici: Capaccio, P; Ottaviani, F; Cuccarini, V; Corbellino, M; Parravicini, C; Menzo, Stefano; Schindler, A; Pignataro, L.
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/83419
Citazioni:
Surgery and topic cidofovir for nasal squamous papillomatosis in HIV+ patient / Capaccio, P., Ottaviani, F., Cuccarini, V., Corbellino, M., Parravicini, C., Menzo, S., Schindler, A., Pignataro, L.. - In: EUROPEAN ARCHIVES OF OTO-RHINO-LARYNGOLOGY. - ISSN 0937-4477. - 266:(2009). [10.1007/s00405-008-0769-7]

Rules-based HIV-1 genotypic resistance interpretation systems predict 8 week and 24 week virological antiretroviral treatment outcome and benefit from drug potency weighting.
Anno: 2009
Rivista: JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
Autori/autrici: Zazzi, M; Prosperi, M; Vicenti, I; DI GIAMBENEDETTO, S; Callegaro, A; Bruzzone, B; Baldanti, F; Gonnelli, A; Boeri, E; Paolini, E; Rusconi, S; Giacometti, Andrea; Maggiolo, F; Menzo, Stefano; DE LUCA, A; ARCA COLLABORATIVE, Group
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/54036
Citazioni:
Rules-based HIV-1 genotypic resistance interpretation systems predict 8 week and 24 week virological antiretroviral treatment outcome and benefit from drug potency weighting / Zazzi, M., Prosperi, M., Vicenti, I., DI GIAMBENEDETTO, S., Callegaro, A., Bruzzone, B., Baldanti, F., Gonnelli, A., Boeri, E., Paolini, E., Rusconi, S., Giacometti, A., Maggiolo, F., Menzo, S., DE LUCA, A., ARCA COLLABORATIVE, G.. - In: JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY. - ISSN 0305-7453. - 64:(2009), pp. 616-624. [10.1093/jac/dkp252]

Molecular epidemiology and pathogenic potential of underdiagnosed human papillomavirus types
Anno: 2008
Rivista: BMC MICROBIOLOGY
Autori/autrici: Menzo, Stefano; Ciavattini, Andrea; Bagnarelli, Patrizia; Marinelli, Katia; Sisti, Stefano; Clementi, Massimo
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/225492
Citazioni:
Molecular epidemiology and pathogenic potential of underdiagnosed human papillomavirus types / Menzo, S., Ciavattini, A., Bagnarelli, P., Marinelli, K., Sisti, S., Clementi, M.. - In: BMC MICROBIOLOGY. - ISSN 1471-2180. - 8:(2008), p. 112. [10.1186/1471-2180-8-112]
Abstract:
Human papillomavirus (HPV) tests are crucial diagnostic tools for the prevention of neoplastic lesions of the uterine cervix. However most commercial methods are designed to detect high-risk (HR) HPV types and a limited selection of low-risk ones, thus missing a fair number of intermediate/low-risk ...types. As a result, many HPV infections remain undiagnosed, generating distrust in virological diagnosis among gynaecologists, who continue to rely preferentially on cytological and colposcopic findings.
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Molecular epidemiology and pathogenetic potential of underdiagnosed human papillomavirus types.
Anno: 2008
Rivista: BMC MICROBIOLOGY
Autori/autrici: Menzo, Stefano; Ciavattini, Andrea; Bagnarelli, Patrizia; Marinelli, K; Sisti, S; Clementi, M.
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/51218
Citazioni:
Molecular epidemiology and pathogenetic potential of underdiagnosed human papillomavirus types / Menzo, S., Ciavattini, A., Bagnarelli, P., Marinelli, K., Sisti, S., Clementi, M.. - In: BMC MICROBIOLOGY. - ISSN 1471-2180. - 8:(2008), p. 112.
Abstract:
Background Human papillomavirus (HPV) tests are crucial diagnostic tools for the prevention of neoplastic lesions of the uterine cervix. However most commercial methods are designed to detect high-risk (HR) HPV types and a limited selection of low-risk ones, thus missing a fair number of intermediat...e/low-risk types. As a result, many HPV infections remain undiagnosed, generating distrust in virological diagnosis among gynaecologists, who continue to rely preferentially on cytological and colposcopic findings. Results In this study, we tested 6,335 consecutive clinical samples, most of them from Italian patients with cytological abnormalities. The samples, collected in 2000-2007, were analyzed using PCR amplification of a 173-206 bp (depending on HPV type) conserved region in the L1 open reading frame, restriction endonuclease analysis and, where required, sequence analysis for type determination. Analysis of a smaller male sample and long term follow-up of a few female subjects was also performed. A total of 2,004 samples tested positive for HPV DNA (32.1%); 21.3% of them were mixed infections. Overall, 59 known and 2 unknown HPV types were detected. Their relative prevalence was calculated; notably, types not clearly identifiable using the most common commercial method accounted for 36% of infections. Clinical findings associated with the underdiagnosed types ranged from H-SIL to low-grade abnormalities, although none of these infections resulted in invasive cancer. Conclusions Given the high prevalence of some underdiagnosed HPV types in the population (principally HPV53, HPV66, HPV84, and HPV87) and their frequent association with cytological abnormalities, techniques capable of detecting and typing them would prove extremely useful.
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HIV-1 replication capacity and genotype changes in patients undergoing treatment interruption or lamivudine monotherapy.
Anno: 2008
Rivista: JOURNAL OF MEDICAL VIROLOGY
Autori/autrici: Gianotti, N; Tiberi, S; Menzo, Stefano; Danise, A; Boeri, E; Galli, L; Clementi, M; Lazzarin, A; Castagna, A.
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/83418
Citazioni:
HIV-1 replication capacity and genotype changes in patients undergoing treatment interruption or lamivudine monotherapy / Gianotti, N., Tiberi, S., Menzo, S., Danise, A., Boeri, E., Galli, L., Clementi, M., Lazzarin, A., Castagna, A.. - In: JOURNAL OF MEDICAL VIROLOGY. - ISSN 0146-6615. - 80:(2008). [10.1002/jmv.21085]

PCR real time assays for the early detection of BKV-DNA in immunocompromised patients.
Anno: 2007
Rivista: NEW MICROBIOLOGICA
Autori/autrici: Marinelli, K; Bagnarelli, Patrizia; Gaffi, G; Trappolini, S; Leoni, Pietro; Paggi, A. M.; DELLA VITTORIA, A; Scalise, G; Varaldo, Pietro; Menzo, Stefano
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/52486
Citazioni:
PCR real time assays for the early detection of BKV-DNA in immunocompromised patients / Marinelli, K; Bagnarelli, Patrizia; Gaffi, G; Trappolini, S; Leoni, Pietro; Paggi, A. M.; DELLA VITTORIA, A; Scalise, G; Varaldo, Pietro; Menzo, Stefano. - In: NEW MICROBIOLOGICA. - ISSN 1121-7138. - 30:(2007), pp. 275-278.

Human papillomavirus infections: new perspectives for prevention and treatment.
Anno: 2007
Rivista: NEW MICROBIOLOGICA
Autori/autrici: Menzo, Stefano; Marinelli, K; Bagnarelli, Patrizia; Rolla, S; Clementi, M.
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/36882
Citazioni:
Human papillomavirus infections: new perspectives for prevention and treatment / Menzo, S., Marinelli, K., Bagnarelli, P., Rolla, S., Clementi, M.. - In: NEW MICROBIOLOGICA. - ISSN 1121-7138. - 30:(2007), pp. 189-212.

Determinants of HIV-1 genotypic resistance to darunavir (TMC114) in a large Italian resistance database (Antiretroviral Resistance Cohort Analysis).
Anno: 2007
Rivista: JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES
Autori/autrici: Rusconi, S; Gianotti, N; Adorni, F; Boeri, E; Menzo, Stefano; Gonnelli, A; Micheli, V; Meraviglia, P; Trezzi, M; Paolini, E; Giacometti, Andrea; Corsi, P; Di Pietro, M; Monno, L; Punzi, G; Zazzi, M; ARCA Collaborative, Group
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/73927
Citazioni:
Determinants of HIV-1 genotypic resistance to darunavir (TMC114) in a large Italian resistance database (Antiretroviral Resistance Cohort Analysis) / Rusconi, S., Gianotti, N., Adorni, F., Boeri, E., Menzo, S., Gonnelli, A., Micheli, V., Meraviglia, P., Trezzi, M., Paolini, E., Giacometti, A., Corsi, P., Di Pietro, M., Monno, L., Punzi, G., Zazzi, M., ARCA Collaborative, G.. - In: JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES. - ISSN 1525-4135. - 46:(2007). [10.1097/QAI.0b013e3181378f73]

Molecular and cellular determinants of cell-to-cell transmission of HCV in vitro.
Anno: 2007
Rivista: JOURNAL OF MEDICAL VIROLOGY
Autori/autrici: Valli, Mb; Crema, A; Lanzilli, G; Serafino, A; Bertolini, L; Ravagnan, G; Ponzetto, A; Menzo, Stefano; Clementi, M; Carloni, G.
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/83417
Citazioni:
Molecular and cellular determinants of cell-to-cell transmission of HCV in vitro / Valli, M.b., Crema, A., Lanzilli, G., Serafino, A., Bertolini, L., Ravagnan, G., Ponzetto, A., Menzo, S., Clementi, M., Carloni, G.. - In: JOURNAL OF MEDICAL VIROLOGY. - ISSN 0146-6615. - 79:(2007). [10.1002/jmv.20947]

Clinicopathological features of primary cutaneous B-cell lymphomas from an academic regional hospital in central Italy: no evidence of Borrelia burgdorferi association
Anno: 2007
Rivista: LEUKEMIA & LYMPHOMA
Autori/autrici: Goteri, Gaia; Ranaldi, R.; Simonetti, Oriana; Capretti, R.; Menzo, Stefano; Stramazzotti, Daniela; Morichetti, D.; Offidani, Annamaria; Rupoli, S.; Leoni, Pietro
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/63707
Citazioni:
Clinicopathological features of primary cutaneous B-cell lymphomas from an academic regional hospital in central Italy: no evidence of Borrelia burgdorferi association / Goteri, G., Ranaldi, R., Simonetti, O., Capretti, R., Menzo, S., Stramazzotti, D., Morichetti, D., Offidani, A., Rupoli, S., Leoni, P.. - In: LEUKEMIA & LYMPHOMA. - ISSN 1042-8194. - 48:11(2007), pp. 2184-2188. [10.1080/10428190701618250]

Infezione da Borrelia Burgdorferi e linfomi cutanei a cellule B nella regione marche
Anno: 2006
Autori/autrici: Simonetti, Oriana; Goteri, Gaia; Rupoli, S.; Ranaldi, R.; Capretti, R.; Menzo, Stefano; Stramazzotti, Daniela; Leoni, Pietro; Offidani, Annamaria
Lingua: ITA
Classificazione IRIS: 4.2 Abstract in Atti di convegno
Scheda completa IRIS: https://iris.univpm.it/handle/11566/81195
Citazioni:
Infezione da Borrelia Burgdorferi e linfomi cutanei a cellule B nella regione marche / Simonetti, O., Goteri, G., Rupoli, S., Ranaldi, R., Capretti, R., Menzo, S., Stramazzotti, D., Leoni, P., Offidani, A.. - (2006), pp. 455-456. (45° Congresso Nazionale ADOI Loreto-Recanati 13-16/09/2006).

No evidence of association between Borrelia Burgdorferi and primary cutaneous B-cell lymphomas in Marche Region, in Italy.
Anno: 2006
Rivista: BLOOD
Autori/autrici: Goteri, Gaia; Capretti, R.; Rupoli, S.; Ranaldi, R.; Menzo, Stefano; Pulini, S.; Tassetti, A.; Morichetti, D.; Scortechini, A. R.; Mulattieri, S.; Stronati, A.; Nicolini, M.; Cicilioni, E. G.; Serresi, S.; Offidani, Annamaria; Leoni, Pietro
Lingua: ENG
Classificazione IRIS: 1.5 Abstract in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/176116
Citazioni:
No evidence of association between Borrelia Burgdorferi and primary cutaneous B-cell lymphomas in Marche Region, in Italy / Goteri, G., Capretti, R., Rupoli, S., Ranaldi, R., Menzo, S., Pulini, S., Tassetti, A., Morichetti, D., Scortechini, A.R., Mulattieri, S., Stronati, A., Nicolini, M., Cicilioni, E.G., Serresi, S., Offidani, A., Leoni, P.. - In: BLOOD. - ISSN 0006-4971. - 108:(2006), pp. 233-233.

Frequency and treatment-related predictors of thymidine-analogue mutation patterns in HIV-1 isolates after unsuccessful antiretroviral therapy.
Anno: 2006
Rivista: THE JOURNAL OF INFECTIOUS DISEASES
Autori/autrici: De Luca, A; Di Giambenedetto, S; Romano, L; Gonnelli, A; Corsi, P; Baldari, M; Di Pietro, M; Menzo, Stefano; Francisci, D; Almi, P; Zazzi, M; Antiretroviral Resistance Cohort Analysis Study, Group
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/83416
Citazioni:
Frequency and treatment-related predictors of thymidine-analogue mutation patterns in HIV-1 isolates after unsuccessful antiretroviral therapy / De Luca, A., Di Giambenedetto, S., Romano, L., Gonnelli, A., Corsi, P., Baldari, M., Di Pietro, M., Menzo, S., Francisci, D., Almi, P., Zazzi, M., Antiretroviral Resistance Cohort Analysis Study, G.. - In: THE JOURNAL OF INFECTIOUS DISEASES. - ISSN 0022-1899. - 193:(2006). [10.1086/502976]

Lamivudine monotherapy in HIV-1-infected patients harbouring a lamivudine-resistant virus: a randomized pilot study (E-184V study).
Anno: 2006
Rivista: AIDS
Autori/autrici: Castagna, A; Danise, A; Menzo, Stefano; Galli, L; Gianotti, N; Carini, E; Boeri, E; Galli, A; Cernuschi, M; Hasson, H; Clementi, M; Lazzarin, A.
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/83415
Citazioni:
Lamivudine monotherapy in HIV-1-infected patients harbouring a lamivudine-resistant virus: a randomized pilot study (E-184V study) / Castagna, A., Danise, A., Menzo, S., Galli, L., Gianotti, N., Carini, E., Boeri, E., Galli, A., Cernuschi, M., Hasson, H., Clementi, M., Lazzarin, A.. - In: AIDS. - ISSN 0269-9370. - 20:(2006). [10.1097/01.aids.0000218542.08845.b2]

Analisi della regione tet(O)-mef(A) in un elemento orf3-negativo di Streptococcus pyogenes.
Anno: 2005
Autori/autrici: Giovanetti, Eleonora; Brenciani, Andrea; Vecchi, M.; Menzo, Stefano; Varaldo, P. E.
Lingua: ITA
Classificazione IRIS: 4.3 Poster
Scheda completa IRIS: https://iris.univpm.it/handle/11566/82954
Citazioni:
Analisi della regione tet(O)-mef(A) in un elemento orf3-negativo di Streptococcus pyogenes / Giovanetti, E., Brenciani, A., Vecchi, M., Menzo, S., Varaldo, P.E.. - (2005).

Eritromicino-resistenza da efflusso in ceppi tetraciclino-sensibili (Tcs) e tetraciclino-resistenti (Tcr) di Streptococcus pyogenes.
Anno: 2004
Autori/autrici: Brenciani, Andrea; Kayode, K. O.; Monachetti, A.; Menzo, Stefano; Roberts, M. C.; Varaldo, P. E.; Giovanetti, Eleonora
Lingua: ITA
Classificazione IRIS: 4.3 Poster
Scheda completa IRIS: https://iris.univpm.it/handle/11566/82955
Citazioni:
Eritromicino-resistenza da efflusso in ceppi tetraciclino-sensibili (Tcs) e tetraciclino-resistenti (Tcr) di Streptococcus pyogenes / Brenciani, A., Kayode, K.O., Monachetti, A., Menzo, S., Roberts, M.C., Varaldo, P.E., Giovanetti, E.. - (2004).

The introduction of the fusion inhibitors in the HAART-regimens.
Anno: 2004
Rivista: NEW MICROBIOLOGICA
Autori/autrici: Hasson, H; Castagna, A; Clementi, M; Menzo, Stefano; Danise, A; Lazzarin, A.
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/83414
Citazioni:
The introduction of the fusion inhibitors in the HAART-regimens / Hasson, H., Castagna, A., Clementi, M., Menzo, S., Danise, A., Lazzarin, A.. - In: NEW MICROBIOLOGICA. - ISSN 1121-7138. - 27:(2004).

Resistance and replicative capacity of HIV-1 strains selected in vivo by long-term enfuvirtide treatment.
Anno: 2004
Rivista: NEW MICROBIOLOGICA
Autori/autrici: Menzo, Stefano; Castagna, A; Monachetti, A; Hasson, H; Danise, A; Carini, E; Bagnarelli, Patrizia; Lazzarin, A; Clementi, M.
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/38131
Citazioni:
Resistance and replicative capacity of HIV-1 strains selected in vivo by long-term enfuvirtide treatment / Menzo, S., Castagna, A., Monachetti, A., Hasson, H., Danise, A., Carini, E., Bagnarelli, P., Lazzarin, A., Clementi, M.. - In: NEW MICROBIOLOGICA. - ISSN 1121-7138. - 27:(2004), pp. 51-61.

Distribution and molecular analysis of mef(A)-containing elements in tetracycline-susceptible and -resistant Streptococcus pyogenes clinical isolates with efflux-mediated erythromycin resistance.
Anno: 2004
Rivista: JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
Autori/autrici: Brenciani, Andrea; OJO K. K., MONACHETTI A; Menzo, Stefano; Roberts, M. C.; Varaldo, Pietro; Giovanetti, Eleonora
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/51133
Citazioni:
Distribution and molecular analysis of mef(A)-containing elements in tetracycline-susceptible and -resistant Streptococcus pyogenes clinical isolates with efflux-mediated erythromycin resistance / Brenciani, A., OJO K. K., M.A., Menzo, S., Roberts, M.C., Varaldo, P., Giovanetti, E.. - In: JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY. - ISSN 0305-7453. - 54:(2004), pp. 991-998. [10.1093/jac/dkh481]
Abstract:
OBJECTIVES: To analyse the distribution and molecular features of mef(A)-containing elements in a large collection of different Streptococcus pyogenes clinical isolates with efflux-mediated erythromycin resistance. To further characterize a tet(O)-mef(A) element. METHODS: Gene detection w...as carried out by PCR using primers designed from established sequences or from sequences in this study. From a tet(O)-mef(A) element (approximately 60 kb), an 11 972 bp region including the tet(O) and mef(A) genes was sequenced. RESULTS: In the tetracycline-susceptible isolates (n =28), the mef(A) gene was contained in a regular Tn1207.1 transposon (7.2 kb), which was inserted into one of two previously described elements, Tn1207.3 (approximately 52 kb) or a 58.8 kb chimeric element, both flanked by the comEC gene. In the tetracycline-resistant isolates (n =61), all of which carried the tet(O) gene, the mef(A) gene was part of a variable Tn1207.1-related transposon inserted into unique elements which contained the tet(O) gene approximately 2.3 to 5.5 kb upstream of the mef(A) gene and were not flanked by the comEC gene. In the Tn1207.1-like transposon of these tet(O)-mef(A) elements, only msr(D) (orf5) and a modified orf6, in addition to mef(A), were detected by PCR in all isolates tested; while orf1 and orf2 were always undetectable, orf3, orf7 and orf8 were found in variable percentages. In an orf3-positive element, sequencing identified four new open reading frames downstream of the tet(O) gene, followed by three short sequences with homology to sequences of the pneumococcal mega element. CONCLUSIONS: The mef(A) gene is carried on different chromosomal genetic elements depending on whether the isolates are susceptible or resistant to tetracycline.
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Immune reconstitution in HIV-1 infected children with discordant response to antiretroviral therapies: patterns of HIV-1 env gene evolution driven by restoration of thymic function.
Anno: 2004
Rivista: NEW MICROBIOLOGICA
Autori/autrici: Vecchi, M; Burighel, N; Bellanova, D; Menzo, Stefano; DE ROSSI, A; Clementi, M; Bagnarelli, Patrizia
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/36884
Citazioni:
Immune reconstitution in HIV-1 infected children with discordant response to antiretroviral therapies: patterns of HIV-1 env gene evolution driven by restoration of thymic function / Vecchi, M., Burighel, N., Bellanova, D., Menzo, S., DE ROSSI, A., Clementi, M., Bagnarelli, P.. - In: NEW MICROBIOLOGICA. - ISSN 1121-7138. - 27:(2004), pp. 99-104.

Intra-host evolution of human immunodeficiency virus type 1 and viral fitness.
Anno: 2004
Rivista: NEW MICROBIOLOGICA
Autori/autrici: Clementi, M; Canducci, F; Bagnarelli, Patrizia; Menzo, Stefano
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/36886
Citazioni:
Intra-host evolution of human immunodeficiency virus type 1 and viral fitness / Clementi, M., Canducci, F., Bagnarelli, P., Menzo, S.. - In: NEW MICROBIOLOGICA. - ISSN 1121-7138. - 27:(2004), pp. 41-44.

Genotypic and phenotypic correlates of the HIV Type 1 env gene evolution in infected children with discordant response to antiretroviral therapy.
Anno: 2004
Rivista: AIDS RESEARCH AND HUMAN RETROVIRUSES
Autori/autrici: Bagnarelli, Patrizia; Vecchi, M; Burighel, N; Bellanova, D; Menzo, Stefano; Clementi, M; DE ROSSI, A.
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/36883
Citazioni:
Genotypic and phenotypic correlates of the HIV Type 1 env gene evolution in infected children with discordant response to antiretroviral therapy / Bagnarelli, P., Vecchi, M., Burighel, N., Bellanova, D., Menzo, S., Clementi, M., DE ROSSI, A.. - In: AIDS RESEARCH AND HUMAN RETROVIRUSES. - ISSN 0889-2229. - 20:(2004), pp. 1306-1313.

Genotype and phenotype patterns of human immunodeficiency virus type 1 resistance to enfuvirtide during long-term treatment.
Anno: 2004
Rivista: ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
Autori/autrici: Menzo, Stefano; Castagna, A; Monachetti, A; Hasson, H; Danise, A; Carini, E; Bagnarelli, Patrizia; Lazzarin, A; Clementi, M.
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/36930
Citazioni:
Genotype and phenotype patterns of human immunodeficiency virus type 1 resistance to enfuvirtide during long-term treatment / Menzo, S., Castagna, A., Monachetti, A., Hasson, H., Danise, A., Carini, E., Bagnarelli, P., Lazzarin, A., Clementi, M.. - In: ANTIMICROBIAL AGENTS AND CHEMOTHERAPY. - ISSN 0066-4804. - 48:(2004), pp. 3253-3259.
Abstract:
The human immunodeficiency virus type 1 (HIV-1) fusion inhibitor enfuvirtide has recently been introduced into clinical practice and has exhibited efficient anti-HIV-1 activity in combination with other antiretroviral agents. In the present study, we addressed the effect of long-term treatment with ...enfuvirtide on the intrahost evolution of HIV-1. The genotype and phenotype patterns and the relative replication capacity (rRC) of enfuvirtide-resistant HIV-1 mutants were evaluated in samples from 11 subjects (7 virological nonresponders and 4 responders) who received the compound for more than 1 year in combination with different regimens. Selection of one or more mutations clustering in a sequence (amino acids 36 to 45) of the gp41 N-terminal heptad repeat was observed in samples from the seven virological nonresponders but not in those from responders. In two subjects who discontinued enfuvirtide, reversion of the resistant genotype was detected within 3 months. Recombinant clones bearing mutated gp41 sequences displayed reduced susceptibilities to enfuvirtide, with the 50% inhibitory concentrations (IC50s) ranging from 0.6 to 12.8 g/ml, whereas the IC50 for isolates with baseline sequences was 0.013 0.010 g/ml. Interestingly, long-term monitoring of resistant variants provided evidence that ongoing adaptation to the drug is paralleled by phenotypic changes. A limited drop in the rRC in the absence of drug was observed for clones from four of the seven nonresponders bearing mutations associated with resistance. Overall, the data indicate that the different genotype patterns associated with a detectable degree of HIV-1 resistance to enfuvirtide generated during long-term treatments are characterized by a substantially low genetic barrier, possible ongoing adaptation with increased degrees of resistance, and limited influence on the viral rRC.
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Analysis of the functional relationship between V3 loop and gp120 context with regard to human immunodeficiency virus coreceptor usage using naturally selected sequences and different viral backbones.
Anno: 2003
Rivista: VIROLOGY
Autori/autrici: Bagnarelli, Patrizia; Fiorelli, L; Vecchi, M; Monachetti, A; Menzo, Stefano; Clementi, M.
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/36541
Citazioni:
Analysis of the functional relationship between V3 loop and gp120 context with regard to human immunodeficiency virus coreceptor usage using naturally selected sequences and different viral backbones / Bagnarelli, P., Fiorelli, L., Vecchi, M., Monachetti, A., Menzo, S., Clementi, M.. - In: VIROLOGY. - ISSN 0042-6822. - 307:(2003), pp. 328-340.

Processivity and drug-dependence of HIV-1 protease: determinants of viral fitness in variants resistant to protease inhibitors.
Anno: 2003
Rivista: AIDS
Autori/autrici: Menzo, Stefano; Monachetti, A; Balotta, C; Corvasce, S; Rusconi, S; Paolucci, S; Baldanti, F; Bagnarelli, Patrizia; Clementi, M.
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/36542
Citazioni:
Processivity and drug-dependence of HIV-1 protease: determinants of viral fitness in variants resistant to protease inhibitors / Menzo, S., Monachetti, A., Balotta, C., Corvasce, S., Rusconi, S., Paolucci, S., Baldanti, F., Bagnarelli, P., Clementi, M.. - In: AIDS. - ISSN 0269-9370. - 17:(2003), pp. 663-671.

Comparison of levels of HIV-1 resistance to protease inhibitors by recombinant versus conventional virus phenotypic assay and two genotypic interpretation procedures in treatment-naive and HAART-experienced HIV-infected patients.
Anno: 2003
Rivista: JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
Autori/autrici: Paolucci, S; Baldanti, F; Zavattoni, M; Comolli, G; Labò, N; Menzo, Stefano; Clementi, M; Gerna, G.
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/83450
Citazioni:
Comparison of levels of HIV-1 resistance to protease inhibitors by recombinant versus conventional virus phenotypic assay and two genotypic interpretation procedures in treatment-naive and HAART-experienced HIV-infected patients / Paolucci, S., Baldanti, F., Zavattoni, M., Comolli, G., Labò, N., Menzo, S., Clementi, M., Gerna, G.. - In: JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY. - ISSN 0305-7453. - 51:(2003).

Italian Patent with european extension: New molecular vector for the geno-phenotypic characteristics of V3 sequences of the HIV gp120.
Anno: 2001
Autori/autrici: Bagnarelli, Patrizia; Clementi, M; Menzo, Stefano
Classificazione IRIS: 6.1 Brevetto
Scheda completa IRIS: https://iris.univpm.it/handle/11566/83480
Citazioni:
Italian Patent with european extension: New molecular vector for the geno-phenotypic characteristics of V3 sequences of the HIV gp120 / Bagnarelli, P., Clementi, M., Menzo, S.. - (2001).

Inhibition of R5X4 dualtropic HIV-1 primary isolates by single chemokine co-receptor ligands.
Anno: 2001
Rivista: VIROLOGY
Autori/autrici: Ghezzi, S; Menzo, Stefano; Brambilla, A; Bordignon, Pp; Lorini, Al; Clementi, M; Poli, G; Vicenzi, E.
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/83448
Citazioni:
Inhibition of R5X4 dualtropic HIV-1 primary isolates by single chemokine co-receptor ligands / Ghezzi, S., Menzo, S., Brambilla, A., Bordignon, P.p., Lorini, A.l., Clementi, M., Poli, G., Vicenzi, E.. - In: VIROLOGY. - ISSN 0042-6822. - 280:(2001). [10.1006/viro.2000.0753]

Identification of six putative novel human papillomaviruses (HPV) and characterization of candidate HPV type 87.
Anno: 2001
Rivista: JOURNAL OF VIROLOGY
Autori/autrici: Menzo, Stefano; Monachetti, A.; Trozzi, C.; Ciavattini, Andrea; Varaldo, Pietro; Clementi, M.
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/53454
Citazioni:
Identification of six putative novel human papillomaviruses (HPV) and characterization of candidate HPV type 87 / Menzo, S., Monachetti, A., Trozzi, C., Ciavattini, A., Varaldo, P., Clementi, M.. - In: JOURNAL OF VIROLOGY. - ISSN 0022-538X. - 75:(2001), pp. 11913-11919.

Quantitative evaluation of the recombinant HIV-1 phenotype to protease inhibitors by a single-step strategy.
Anno: 2000
Rivista: AIDS
Autori/autrici: Menzo, Stefano; Rusconi, S.; Monachetti, A.; Colombo, M. C.; Violin, M.; Bagnarelli, Patrizia; P., Varaldo; Moroni, M.; Galli, M.; Balotta, C.; Clementi, M.
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/28187
Citazioni:
Quantitative evaluation of the recombinant HIV-1 phenotype to protease inhibitors by a single-step strategy / Menzo, Stefano; Rusconi, S.; Monachetti, A.; Colombo, M. C.; Violin, M.; Bagnarelli, Patrizia; P., Varaldo; Moroni, M.; Galli, M.; Balotta, C.; Clementi, M.. - In: AIDS. - ISSN 0269-9370. - 14:(2000), pp. 1101-1110.

Viral dynamics and mutations in the pol gene during primary HIV-1 infection.
Anno: 2000
Rivista: JOURNAL OF BIOLOGICAL REGULATORS & HOMEOSTATIC AGENTS
Autori/autrici: Cenci, A; Bertoli, A; Tambussi, G; Menzo, Stefano; Clementi, M; Lazzarin, A; Capiluppi, B; Ciuffreda, D; Forbici, F; Aquaro, S; Marcuccilli, F; Caliò, R; Perno, Cf
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/83445
Citazioni:
Viral dynamics and mutations in the pol gene during primary HIV-1 infection / Cenci, A., Bertoli, A., Tambussi, G., Menzo, S., Clementi, M., Lazzarin, A., Capiluppi, B., Ciuffreda, D., Forbici, F., Aquaro, S., Marcuccilli, F., Caliò, R., Perno, C.f.. - In: JOURNAL OF BIOLOGICAL REGULATORS & HOMEOSTATIC AGENTS. - ISSN 0393-974X. - 14:(2000).

Absence of HHV-6 and HHV-7 in cerebrospinal fluid in relapsing-remitting multiple sclerosis.
Anno: 2000
Rivista: ACTA NEUROLOGICA SCANDINAVICA
Autori/autrici: Taus, C; Pucci, E; Cartechini, E; Fié, A; Giuliani, G; Clementi, M; Menzo, Stefano
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/83446
Citazioni:
Absence of HHV-6 and HHV-7 in cerebrospinal fluid in relapsing-remitting multiple sclerosis / Taus, C., Pucci, E., Cartechini, E., Fié, A., Giuliani, G., Clementi, M., Menzo, S.. - In: ACTA NEUROLOGICA SCANDINAVICA. - ISSN 0001-6314. - 101:(2000).

Lack of Chlamydia infection of the central nervous system in multiple sclerosis.
Anno: 2000
Rivista: ANNALS OF NEUROLOGY
Autori/autrici: Pucci, E; Taus, C; Cartechini, E; Morelli, M; Giuliani, G; Clementi, M; Menzo, Stefano
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/83447
Citazioni:
Lack of Chlamydia infection of the central nervous system in multiple sclerosis / Pucci, E., Taus, C., Cartechini, E., Morelli, M., Giuliani, G., Clementi, M., Menzo, S.. - In: ANNALS OF NEUROLOGY. - ISSN 0364-5134. - 48:(2000).

Complexity and dynamics of HIV-1 quasispecies.
Anno: 2000
Rivista: JOURNAL OF BIOLOGICAL REGULATORS & HOMEOSTATIC AGENTS
Autori/autrici: Menzo, Stefano; Bagnarelli, Patrizia; Monachetti, A; Fiorelli, L; Clementi, M.
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/36373
Citazioni:
Complexity and dynamics of HIV-1 quasispecies / Menzo, S., Bagnarelli, P., Monachetti, A., Fiorelli, L., Clementi, M.. - In: JOURNAL OF BIOLOGICAL REGULATORS & HOMEOSTATIC AGENTS. - ISSN 0393-974X. - 14:(2000), pp. 4-6.

European Patent n° EPO 99830776.3: Method to evaluate the HIV drug sensitivity
Anno: 1999
Autori/autrici: Menzo, Stefano; Clementi, M.
Lingua: ENG
Classificazione IRIS: 6.1 Brevetto
Scheda completa IRIS: https://iris.univpm.it/handle/11566/83476
Citazioni:
European Patent n° EPO 99830776.3: Method to evaluate the HIV drug sensitivity / Menzo, S., Clementi, M.. - (1999).

Host-specific modulation of the selective constraints driving human immunodeficiency virus type 1 env gene evolution.
Anno: 1999
Rivista: JOURNAL OF VIROLOGY
Autori/autrici: Bagnarelli, Patrizia; Mazzola, F; Menzo, Stefano; Montroni, M; Butini, L; Clementi, M.
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/36547
Citazioni:
Host-specific modulation of the selective constraints driving human immunodeficiency virus type 1 env gene evolution / Bagnarelli, P., Mazzola, F., Menzo, S., Montroni, M., Butini, L., Clementi, M.. - In: JOURNAL OF VIROLOGY. - ISSN 0022-538X. - 73:(1999), pp. 3764-3777.

Rare mutations in a domain crucial for V3-loop structure prevail in replicating HIV from long-term non-progressors.
Anno: 1998
Rivista: AIDS
Autori/autrici: Menzo, Stefano; Sampaolesi, R; Vicenzi, E; Santagostino, E; Liuzzi, G; Chirianni, A; Piazza, M; Cohen, Oj; Bagnarelli, Patrizia; Clementi, M.
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/36549
Citazioni:
Rare mutations in a domain crucial for V3-loop structure prevail in replicating HIV from long-term non-progressors / Menzo, S., Sampaolesi, R., Vicenzi, E., Santagostino, E., Liuzzi, G., Chirianni, A., Piazza, M., Cohen, O.j., Bagnarelli, P., Clementi, M.. - In: AIDS. - ISSN 0269-9370. - 12:(1998), pp. 985-997.

The role of human papillomavirus DNAs in cervical carcinoma and risk of lymph node metastasis: association with 72-kilodalton metalloproteinase immunostaining
Anno: 1998
Rivista: CANCER
Autori/autrici: Garzetti, G. G.; Ciavattini, Andrea; Lucarini, Guendalina; Goteri, Gaia; Menzo, Stefano; De Nictolis, M.; Romanini, C.; Biagini, G.
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/52414
Citazioni:
The role of human papillomavirus DNAs in cervical carcinoma and risk of lymph node metastasis: association with 72-kilodalton metalloproteinase immunostaining / Garzetti, G.G., Ciavattini, A., Lucarini, G., Goteri, G., Menzo, S., De Nictolis, M., Romanini, C., Biagini, G.. - In: CANCER. - ISSN 0008-543X. - 82:5(1998), pp. 886-892.
Abstract:
The role of human papillomavirus (HPV) as a prognostic factor in cervical carcinoma is not understood completely and little is known regarding the intrinsic mechanisms involved in the metastatic process of HPV positive carcinoma. The authors evaluated HPV status with respect to clinical features in ...early stage cervical carcinoma, with special emphasis on lymph node spread. The authors also analyzed the relation between HPV, lymph node involvement, and 72-kilodalton (kDa) metalloproteinase immunostaining, an enzyme that cleaves Type IV collagen and may play a role in tumor metastasis.
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Selected nucleotide sequence of the pol gene of the monocytotropic strain HIV type 1 BaL.
Anno: 1997
Rivista: AIDS RESEARCH AND HUMAN RETROVIRUSES
Autori/autrici: Cenci, A; Perno, Cf; Menzo, Stefano; Clementi, M; Erba, F; Tavazzi, B; Di Pierro, D; Aquaro, S; Caliò, R.
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/83444
Citazioni:
Selected nucleotide sequence of the pol gene of the monocytotropic strain HIV type 1 BaL / Cenci, A., Perno, C.f., Menzo, S., Clementi, M., Erba, F., Tavazzi, B., Di Pierro, D., Aquaro, S., Caliò, R.. - In: AIDS RESEARCH AND HUMAN RETROVIRUSES. - ISSN 0889-2229. - 13:(1997).

Molecular monitoring of human immunodeficiency virus type 1 infection.
Anno: 1997
Rivista: JOURNAL OF BIOLOGICAL REGULATORS & HOMEOSTATIC AGENTS
Autori/autrici: Clementi, M; Bagnarelli, Patrizia; Menzo, Stefano; Cardelli, M; Mazzola, F; Sampaolesi, R.
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/36629
Citazioni:
Molecular monitoring of human immunodeficiency virus type 1 infection / Clementi, M., Bagnarelli, P., Menzo, S., Cardelli, M., Mazzola, F., Sampaolesi, R.. - In: JOURNAL OF BIOLOGICAL REGULATORS & HOMEOSTATIC AGENTS. - ISSN 0393-974X. - 11:(1997), pp. 64-68.

Dynamics and modulation of human immunodeficiency virus type 1 transcripts in vitro and in vivo
Anno: 1996
Rivista: JOURNAL OF VIROLOGY
Autori/autrici: Bagnarelli, Patrizia; Valenza, A.; Menzo, Stefano; Sampaolesi, R.; Varaldo, Pietro; Butini, L.; Montroni, M.; Perno, C. F.; Aquaro, S.; Mathéz, D.; Leibowitch, J.; Balotta, C.; Clementi, M.
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/71090
Citazioni:
Dynamics and modulation of human immunodeficiency virus type 1 transcripts in vitro and in vivo / Bagnarelli, Patrizia; Valenza, A.; Menzo, Stefano; Sampaolesi, R.; Varaldo, Pietro; Butini, L.; Montroni, M.; Perno, C. F.; Aquaro, S.; Mathéz, D.; Leibowitch, J.; Balotta, C.; Clementi, M.. - In: JOURNAL OF VIROLOGY. - ISSN 0022-538X. - 70:(1996), pp. 7603-7613.
Abstract:
The dynamics of human immunodeficiency virus type 1 (HIV-1) transcription was analyzed in vitro and in vivo by using a specific molecular approach which allows accurate quantitation of the different classes of viral mRNAs. Unspliced (US) and multiply spliced (MS) HIV-1 transcripts were assayed by co...mpetitive reverse transcription (cRT)-PCR, using a single competitor RNA bearing in tandem internally deleted sequences of both template species. Acute HIV-1 infection of primary peripheral blood mononuclear cells (PBMCs), monocytes/macrophages cells, and the A3.01 T-lymphocyte-derived cell line was studied; both classes of HIV-1 mRNAs increased exponentially (r2 > 0.98) at days 1 to 3 and 1 to 4 postinfection in HIV(IIIB)-infected A3.01 cells and PBMCs, respectively, whereas monocytes/macrophages infected with monocytotropic HIV(BaL) exhibited a linear (r2 = 0.81 to 0.94) accumulation of US and MS transcripts. Following induction of chronically infected ACH-2 cells, MS transcripts increased 2 h postinduction and peaked at 5 h (doubling time, 58 min), while at 24 h, US mRNAs increased 3,053-fold compared with basal time (doubling time, 137 min). To address the biopathological significance of HIV-1 expression pattern during infection progression, pilot cross-sectional and longitudinal analyses were carried out with samples from untreated and treated HIV-1-infected patients. In almost all untreated (recently infected, long-term nonprogressor, and progressor) patients, MS transcript levels followed the general trend of systemic HIV-1 activity. In patients under treatment with powerful antiretroviral compounds, viral MS transcripts rapidly fell to undetectable levels, indicating that in vivo, levels of MS mRNAs in PBMCs are closely associated with the number of newly infected cells and suggesting a new role for the quantitative analysis of HIV-1 transcription in infected patients.
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Clinical use of quantitative molecular methods in studying human immunodeficiency virus type 1 (HIV-1) infection
Anno: 1996
Rivista: CLINICAL MICROBIOLOGY REVIEWS
Autori/autrici: Clementi, M.; Menzo, Stefano; Bagnarelli, Patrizia; Valenza, A.; Paolucci, S.; Sampaolesi, R.; Manzin, A.; Varaldo, Pietro
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/71087
Citazioni:
Clinical use of quantitative molecular methods in studying human immunodeficiency virus type 1 (HIV-1) infection / Clementi, M., Menzo, S., Bagnarelli, P., Valenza, A., Paolucci, S., Sampaolesi, R., Manzin, A., Varaldo, P.. - In: CLINICAL MICROBIOLOGY REVIEWS. - ISSN 0893-8512. - 9:(1996), pp. 135-147.

Quantitative molecular monitoring of human immunodeficiency virus type 1 activity during therapy with specific antiretroviral compounds
Anno: 1995
Rivista: JOURNAL OF CLINICAL MICROBIOLOGY
Autori/autrici: Bagnarelli, Patrizia; Menzo, Stefano; Valenza, A.; Paolucci, S.; Petroni, S.; Scalise, G.; Sampaolesi, R.; Manzin, A.; Varaldo, Pietro; Clementi, M.
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/71084
Citazioni:
Quantitative molecular monitoring of human immunodeficiency virus type 1 activity during therapy with specific antiretroviral compounds / Bagnarelli, Patrizia; Menzo, Stefano; Valenza, A.; Paolucci, S.; Petroni, S.; Scalise, G.; Sampaolesi, R.; Manzin, A.; Varaldo, Pietro; Clementi, M.. - In: JOURNAL OF CLINICAL MICROBIOLOGY. - ISSN 0095-1137. - 33:(1995), pp. 16-23.

HPV DNA positivity and natural killer cell activity in the clinical outcome of mild cervical dysplasia: integration between virus and immune system
Anno: 1995
Rivista: GYNECOLOGIC AND OBSTETRIC INVESTIGATION
Autori/autrici: Garzetti, G. G.; Ciavattini, Andrea; Goteri, Gaia; De Nictolis, M.; Menzo, Stefano; Muzzioli, M.; Fabris, N.
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/63730
Citazioni:
HPV DNA positivity and natural killer cell activity in the clinical outcome of mild cervical dysplasia: integration between virus and immune system / Garzetti, G.G., Ciavattini, A., Goteri, G., De Nictolis, M., Menzo, S., Muzzioli, M., Fabris, N.. - In: GYNECOLOGIC AND OBSTETRIC INVESTIGATION. - ISSN 0378-7346. - 39:2(1995), pp. 130-135.
Abstract:
The objective was to examine the prevalence of human papillomavirus (HPV) DNA infection in mild cervical dysplasia and to evaluate longitudinally the persistence of HPV DNA positivity in an observational study, aiming at identifying the role of peripheral blood lymphocyte natural killer activity in ...the natural history of dysplastic disease. Twenty-three patients with histologically proven mild cervical dysplasia were selected. The HPV DNA positivity, determined by polymerase chain reaction, and cervical dysplasia were monitored cytologically and colposcopically at the 3rd (time 1), 6th (time 2) and 12th months (time 3), and defined by biopsies for routine histology taken at times 2 and 3. For each patient included in the study, the immune reactivity was evaluated at the time of diagnosis and afterwards, longitudinally during the follow-up. The immune status analysis included T lymphocyte subsets (CD3, CD4, CD8, CD56, CD16 monoclonal antibodies by Beckton Dickinson, Mountain View, Calif., USA) and determinations of natural killer cell activity (against the sensitive cell line K 562). Eighteen out of the 23 women with mild cervical dysplasia (78.3%) were found positive for HPV DNA, with a significantly high representation of HPV DNA type 16 (55.6% of cases). At the end of the study, 12 out of 18 HPV-DNA-positive women became negative (defined by two or more negative tests) for the original HPV DNA type, with 66.7% of spontaneous HPV DNA negativization rate (p = 0.6).(ABSTRACT TRUNCATED AT 250 WORDS)
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Quantitative molecular methods in virology.
Anno: 1995
Rivista: ARCHIVES OF VIROLOGY
Autori/autrici: Clementi, M; Menzo, Stefano; Manzin, A; Bagnarelli, Patrizia
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/36797
Citazioni:
Quantitative molecular methods in virology / Clementi, M., Menzo, S., Manzin, A., Bagnarelli, P.. - In: ARCHIVES OF VIROLOGY. - ISSN 0304-8608. - 140:(1995), pp. 1523-1539.

Studies in subjects with long-term nonprogressive human immunodeficiency virus infection.
Anno: 1995
Rivista: NEW ENGLAND JOURNAL OF MEDICINE
Autori/autrici: Pantaleo, G; Menzo, Stefano; Vaccarezza, M; Graziosi, C; Cohen, Oj; Demarest, Jf; Montefiori, D; Orenstein, Jm; Fox, C; Schrager, Lk
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/83443
Citazioni:
Studies in subjects with long-term nonprogressive human immunodeficiency virus infection / Pantaleo, G., Menzo, S., Vaccarezza, M., Graziosi, C., Cohen, O.j., Demarest, J.f., Montefiori, D., Orenstein, J.m., Fox, C., Schrager, L.k.. - In: THE NEW ENGLAND JOURNAL OF MEDICINE. - ISSN 0028-4793. - 332:(1995). [10.1056/NEJM199501263320402]

Competitive polymerase chain reaction for quantitating feline immunodeficiency virus load in infected cat tissues.
Anno: 1994
Rivista: MOLECULAR AND CELLULAR PROBES
Autori/autrici: Pistello, M; Menzo, Stefano; Giorgi, M; Da Prato, L; Cammarota, G; Clementi, M; Bendinelli, M.
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/83442
Citazioni:
Competitive polymerase chain reaction for quantitating feline immunodeficiency virus load in infected cat tissues / Pistello, M., Menzo, S., Giorgi, M., Da Prato, L., Cammarota, G., Clementi, M., Bendinelli, M.. - In: MOLECULAR AND CELLULAR PROBES. - ISSN 0890-8508. - 8:(1994). [10.1006/mcpr.1994.1032]

Quantitation of hepatitis C virus genome molecules in plasma samples.
Anno: 1994
Rivista: JOURNAL OF CLINICAL MICROBIOLOGY
Autori/autrici: Manzin, A; Bagnarelli, Patrizia; Menzo, Stefano; Giostra, F; Brugia, M; Francesconi, R; Bianchi, Fb; Clementi, M.
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/36635
Citazioni:
Quantitation of hepatitis C virus genome molecules in plasma samples / Manzin, A., Bagnarelli, P., Menzo, S., Giostra, F., Brugia, M., Francesconi, R., Bianchi, F.b., Clementi, M.. - In: JOURNAL OF CLINICAL MICROBIOLOGY. - ISSN 0095-1137. - 32:(1994), pp. 1939-1944.