Late or missed HIV diagnosis during pregnancy is still occurring in a high-income country and represents a high risk of MTCT
Anno: 2026
Rivista: INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
Autori/autrici: Pinnetti, Carmela; Marelli, Cristina; Ranzani, Alice; Mazzotta, Valentina; Taramasso, Lucia; Cirioni, Oscar; Costantini, Andrea; Menzaghi, Barbara; Vecchio, Rosa Fontana Del; Girardi, Enrico; Saracino, Annalisa; Cingolani, Antonella; Biagio, Antonio Di; Mussini, Cristina; Monforte, Antonella D'Arminio; Antinori, Andrea
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/357993
Citazioni:
Late or missed HIV diagnosis during pregnancy is still occurring in a high-income country and represents a high risk of MTCT / Pinnetti, C., Marelli, C., Ranzani, A., Mazzotta, V., Taramasso, L., Cirioni, O., Costantini, A., Menzaghi, B., Vecchio, R.F.D., Girardi, E., Saracino, A., Cingolani, A., Biagio, A.D., Mussini, C., Monforte, A.D., Antinori, A.. - In: INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES. - ISSN 1201-9712. - STAMPA. - 168:(2026). [10.1016/j.ijid.2026.108700]
Abstract:
Objectives: Integrated strategies for early HIV diagnosis and treatment among pregnant women with HIV (pWWH) have reduced mother-to-child transmission (MTCT) to below 2%. We aim to evaluate pregnancy outcomes and antiretroviral therapy (ART) changes in pWWH during pregnancy planning and delivery. Me...thods: We included pWWH enrolled in the ICONA Cohort in 2011-2024. Wilcoxon rank-sum and Chi-squared or Fisher's exact tests described population characteristics. ART modifications, maternal immunovirological status, and MTCT rates were analyzed. Multivariable regression analysis assessed the likelihood of viral suppression at delivery. Results: 419 pregnancies in 311 pWWH were evaluated; outcomes were available for 333 pregnancies (100 ART-naive, 233 ART-experienced) in 255 pWWH: 267 live-born births (80%), 35 miscarriages, 28 voluntary interruptions, 2 stillbirths, and 1 intrauterine death. HIV was diagnosed during pregnancy in 83 women. ART changes occurred in 29.6% of ART-naïve during pregnancy. Among ART-experienced, 6% changed in the six months before pregnancy, 25.8% during the first trimester, and 4.7% afterward. Tenofovir disoproxil/emtricitabine was the most used backbone. A significant proportion of pregnancies were exposed to PIs, but use of INSTIs increased over time. Caesarean section occurred in 44.4%, vaginal deliveries in 24.9%. Two HIV-positive newborns were observed (1.1%). Conclusion: Despite improved viral suppression, late or missed HIV diagnoses during pregnancy continue to drive MTCT. Strengthening early HIV testing, antenatal care access, and retention in care is critical to eliminate perinatal transmission.
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In vitro activities of lipopeptides against fluconazole-resistant Candida auris
Anno: 2025
Rivista: MICROBIOLOGY SPECTRUM
Autori/autrici: Fioriti, Simona; Pallotta, Francesco; D'Achille, Gloria; Cirioni, Oscar; Simonetti, Oriana; Neubauer, Damian; Kamysz, Elzbieta; Kamysz, Wojciech; Brescini, Lucia; Caucci, Sara; Caggiano, Giuseppina; Giacometti, Andrea; Morroni, Gianluca; Barchiesi, Francesco
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/348062
Citazioni:
In vitro activities of lipopeptides against fluconazole-resistant Candida auris / Fioriti, S., Pallotta, F., D'Achille, G., Cirioni, O., Simonetti, O., Neubauer, D., Kamysz, E., Kamysz, W., Brescini, L., Caucci, S., Caggiano, G., Giacometti, A., Morroni, G., Barchiesi, F.. - In: MICROBIOLOGY SPECTRUM. - ISSN 2165-0497. - 13:4(2025). [10.1128/spectrum.01786-24]
Abstract:
Candida auris has increasingly become a global threat due to its wide range of antifungal resistances as well as its ability to sustain outbreaks in clinical settings. Two lipopeptides, C14-NleRR-NH2 (Nel) and C14-WRR-NH2 (WR), were evaluated against six fluconazole-resistant C. auris isolates. Both... molecules showed good antimicrobial activity as demonstrated by MIC determination, time-kill, and microscopy experiments. The peptides were able to inhibit fungal growth, while sub-MIC concentrations of the molecules delayed the growth. Moreover, the combinations of the two peptides with fluconazole demonstrated a reciprocal potentiation by checkerboard and time-kill experiments. Our results showed that antimicrobial peptides could be a promising option for the treatment of antifungal-resistant C. auris.
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Clonal dissemination of Klebsiella pneumoniae carrying blaOXA-48 gene in a central Italy hospital
Anno: 2024
Rivista: JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
Autori/autrici: D'Achille, Gloria; Nunzi, Ilaria; Fioriti, Simona; Cirioni, Oscar; Brescini, Lucia; Giacometti, Andrea; Teodori, Lucia; Brenciani, Andrea; Giovanetti, Eleonora; Mingoia, Marina; Morroni, Gianluca
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/337554
Citazioni:
Clonal dissemination of Klebsiella pneumoniae carrying blaOXA-48 gene in a central Italy hospital / D'Achille, G., Nunzi, I., Fioriti, S., Cirioni, O., Brescini, L., Giacometti, A., Teodori, L., Brenciani, A., Giovanetti, E., Mingoia, M., Morroni, G.. - In: JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE. - ISSN 2213-7173. - 38:(2024), pp. 339-340. [10.1016/j.jgar.2024.07.004]

ER-mitochondria association negatively affects wound healing by regulating NLRP3 activation
Anno: 2024
Rivista: CELL DEATH & DISEASE
Autori/autrici: Licini, Caterina; Morroni, Gianluca; Lucarini, Guendalina; Vitto, Veronica Angela Maria; Orlando, Fiorenza; Missiroli, Sonia; D'Achille, Gloria; Perrone, Mariasole; Spadoni, Tatiana; Graciotti, Laura; Bigossi, Giorgia; Provinciali, Mauro; Offidani, Annamaria; Mattioli-Belmonte, Monica; Cirioni, Oscar; Pinton, Paolo; Simonetti, Oriana; Marchi, Saverio
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/337555
Citazioni:
ER-mitochondria association negatively affects wound healing by regulating NLRP3 activation / Licini, C., Morroni, G., Lucarini, G., Vitto, V.A.M., Orlando, F., Missiroli, S., D'Achille, G., Perrone, M., Spadoni, T., Graciotti, L., Bigossi, G., Provinciali, M., Offidani, A., Mattioli-Belmonte, M., Cirioni, O., Pinton, P., Simonetti, O., Marchi, S.. - In: CELL DEATH & DISEASE. - ISSN 2041-4889. - 15:6(2024). [10.1038/s41419-024-06765-9]
Abstract:
Methicillin-resistant Staphylococcus aureus (MRSA) is the most common causative agent of acute bacterial skin and skin-structure infections (ABSSSI), one of the major challenges to the health system worldwide. Although the use of antibiotics as the first line of intervention for MRSA-infected wounds... is recommended, important side effects could occur, including cytotoxicity or immune dysregulation, thus affecting the repair process. Here, we show that the oxazolidinone antibiotic linezolid (LZD) impairs wound healing by aberrantly increasing interleukin 1 beta (IL-1 beta) production in keratinocytes. Mechanistically, LZD triggers a reactive oxygen species (ROS)-independent mitochondrial damage that culminates in increased tethering between the endoplasmic reticulum (ER) and mitochondria, which in turn activates the NLR family pyrin domain-containing 3 (NLRP3) inflammasome complex by promoting its assembly to the mitochondrial surface. Downregulation of ER-mitochondria contact formation is sufficient to inhibit the LZD-driven NLRP3 inflammasome activation and IL-1 beta production, restoring wound closure. These results identify the ER-mitochondria association as a key factor for NLRP3 activation and reveal a new mechanism in the regulation of the wound healing process that might be clinically relevant
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Late or missed HIV diagnosis during pregnancy is still occurring in a high-income country and represents a high risk of MTCT
Anno: 2024
Rivista: JOURNAL OF THE INTERNATIONAL AIDS SOCIETY
Autori/autrici: Pinnetti, Carmela; Marelli, Cristina; Ranzani, Alice; Mazzotta, Valentina; Taramasso, Lucia; Cirioni, Oscar; Costantini, Andrea; Menzaghi, Barbara; Fontana Del Vecchio, Rosa; Girardi, Enrico; Saracino, Annalisa; Cingolani, Antonella; Di Biagio, Antonio; d’Arminio Monforte, Antonella; Antinori, Andrea
Lingua: ENG
Classificazione IRIS: 1.5 Abstract in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/338972
Citazioni:
Late or missed HIV diagnosis during pregnancy is still occurring in a high-income country and represents a high risk of MTCT / Pinnetti, C., Marelli, C., Ranzani, A., Mazzotta, V., Taramasso, L., Cirioni, O., Costantini, A., Menzaghi, B., Fontana Del Vecchio, R., Girardi, E., Saracino, A., Cingolani, A., Di Biagio, A., d’Arminio Monforte, A., Antinori, A.. - In: JOURNAL OF THE INTERNATIONAL AIDS SOCIETY. - ISSN 1758-2652. - STAMPA. - 27:(2024), pp. P006.26-P006.27. [10.1002/jia2.26370]
Abstract:
Background: Among pregnant women with HIV (pWWH), integrated strategies of early diagnosis and treatment during pregnancy and delivery have resulted in MTCT reduction to <2%. We aim to analyse therapy changes in planning and during pregnancy and investigate pregnancy outcomes. Materials and m...ethods: We included all pWWH enrolled in ICONA cohort in 2011–2024. Chi-squared and Wilcoxon rank-sum test were used to describe population characteristics. Changes in ART, maternal immunovirological status and MTCT were analysed. Results: Four hundred and three pregnancies in 293 pWWH were evaluated. Outcomes were available in 333 pregnancies (95 naives, 238 ART-experienced) of 262 pWWH: 38 miscarriages, 31 voluntary pregnancy interruptions and 264 full-term births (two intrauterine deaths). Characteristics of pWWH are summarized (Table 1). Seventy-one women were diagnosed with HIV while pregnant (38 first, 23 second, 10 third trimester) and started ART at median gestational (IQR) time of 15 weeks (13–21). Eleven women received diagnosis and ART post-partum. Foreign-born pWWH had the same chance of Italian-borns of being diagnosed late or after pregnancy (15/171 vs. 6/91, p = 0.54). Among the ART-experienced, 76/238 (32.0%) switched ART 6 months before pregnancy or in the first trimester: dolutegravir was interrupted in 3/101 (3%), cobicistat in 15/114 (13%) and tenofovir alafenamide in 26/195 (13%). Tenofovir disoproxil/emtricitabine remained the most common backbone in pregnancy (112/264, 42%). At delivery, a substantial proportion of pWWH received protease inhibitors (32%) and integrase strand transfer inhibitor (23%). The proportion of women achieving viral suppression by the third trimester was 84% in 2011–2015 and 93% in 2016–2024; p < 0.02. Caesarean section occurred in 57.2%, and vaginal deliveries in 32.9% (no data in 9.8%). We observed two HIV-positive newborns (0.8%): the first from a mother diagnosed late during pregnancy and starting ART shortly before delivery (29 weeks) without obtaining HIV-RNA undetectability, the second in a woman lost to follow-up during pregnancy and returned to care after delivery. Both pWWH were born outside Italy. Conclusions: HIV diagnosis occurred late during pregnancy or was missed even after delivery in 21/264 (8.0%) of pWWH, resulting in two cases of MTCT. Interventions are still needed to improve access to antenatal care and HIV testing, especially for foreign-born women.
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The sources of antimicrobial peptides against Gram-positives and Gramnegatives: our research experience
Anno: 2023
Rivista: LE INFEZIONI IN MEDICINA
Autori/autrici: Rizzetto, Giulio; Gambini, Daisy; Maurizi, Andrea; Molinelli, Elisa; De Simoni, Edoardo; Pallotta, Francesco; Brescini, Lucia; Cirioni, Oscar; Offidani, Annamaria; Simonetti, Oriana; Giacometti, Andrea
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/322354
Citazioni:
The sources of antimicrobial peptides against Gram-positives and Gramnegatives: our research experience / Rizzetto, G., Gambini, D., Maurizi, A., Molinelli, E., De Simoni, E., Pallotta, F., Brescini, L., Cirioni, O., Offidani, A., Simonetti, O., Giacometti, A.. - In: LE INFEZIONI IN MEDICINA. - ISSN 2532-8689. - 31:3(2023), pp. 306-322. [10.53854/liim-3103-5]
Abstract:
: Antibiotic resistance of Gram-positive and Gramnegative bacteria is becoming increasingly prevalent. For this reason, the search for new molecules that can overcome current resistance and also recover antibiotics that are no longer effective is becoming increasingly urgent. Our research group at t...he 'Polytechnic University of Marche' managed to study the effectiveness of certain antimicrobial peptides (AMPs). We decided to review our experience with AMPs by classifying them according to their origin and evaluating their effect on Gram-negative and Gram-positive bacteria. AMPs can derive from mammals, amphibians, microorganisms, and insects. In conclusion, our research experience shows that the richest source of AMPs are amphibians. However, the studies done are mainly in vitro or in animal models, requiring further human studies to assess the efficacy and safety of these molecules. AMPs may be a new therapeutic option for infections sustained by multi-resistant micro-organisms and for overcoming the mechanisms of resistance to antibiotics currently used. In particular, combining AMPs with antibiotics, including those with limited antimicrobial activity due to antimicrobial resistance, has often shown a synergistic effect, increasing or restoring their efficacy. The possibility of using manageable and relatively safe antibiotics again is crucial, considering the widespread increase in bacterial resistance in hospitals and the community. Despite a plethora of research on AMPs and their application as potential treatment on infectious diseases, this area needs further exploration. There is evidence that the characteristics of AMPs can seriously improve through structural chemical modifications and different delivery systems to become alternatives drugs to conventional antibiotics. The aim is to provide an overview of the possible sources from which AMPs are extracted, evaluating their action exclusively on Gram-positive and negative bacteria. This is to determine, based on our experience, which might be the most promising sources of AMPs for future research as well.
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Genomic Analysis of a Linezolid-Resistant Staphylococcus capitis Causing Bacteremia: Report from a University Hospital in Central Italy
Anno: 2023
Rivista: MICROBIAL DRUG RESISTANCE
Autori/autrici: Brescini, Lucia; Fioriti, Simona; Coccitto, Sonia N; Cinthi, Marzia; Mingoia, Marina; Cirioni, Oscar; Giacometti, Andrea; Giovanetti, Eleonora; Morroni, Gianluca; Brenciani, Andrea
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/316655
Citazioni:
Genomic Analysis of a Linezolid-Resistant Staphylococcus capitis Causing Bacteremia: Report from a University Hospital in Central Italy / Brescini, L., Fioriti, S., Coccitto, S.N., Cinthi, M., Mingoia, M., Cirioni, O., Giacometti, A., Giovanetti, E., Morroni, G., Brenciani, A.. - In: MICROBIAL DRUG RESISTANCE. - ISSN 1076-6294. - (2023). [10.1089/mdr.2022.0330]
Abstract:
Although coagulase negative staphylococci are rarely associated with complicated diseases, in some cases they cause life-threatening infections. Here we described a clinical case of a bacteremia due to a methicillin- and linezolid-resistant Staphylococcus capitis in a patient previously treated with... linezolid. Whole genome sequencing revealed the common mutation G2576T in all rDNA 23S alleles and several acquired resistance genes. Moreover, the isolate was epidemiologically distant from the NRCS-A clade, usually responsible for nosocomial infections in neonatal intensive care units. Our findings further confirm the ability of minor staphylococci to acquire antibiotic resistances and challenge the treatment of these infections.
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MRSA and Skin Infections in Psoriatic Patients: Therapeutic Options and New Perspectives
Anno: 2022
Rivista: ANTIBIOTICS
Autori/autrici: Rizzetto, Giulio; Molinelli, Elisa; Radi, Giulia; Cirioni, Oscar; Brescini, Lucia; Giacometti, Andrea; Offidani, Annamaria; Simonetti, Oriana
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/315733
Citazioni:
MRSA and Skin Infections in Psoriatic Patients: Therapeutic Options and New Perspectives / Rizzetto, G., Molinelli, E., Radi, G., Cirioni, O., Brescini, L., Giacometti, A., Offidani, A., Simonetti, O.. - In: ANTIBIOTICS. - ISSN 2079-6382. - ELETTRONICO. - 11:11(2022), p. 1504. [10.3390/antibiotics11111504]
Abstract:
Psoriatic patients present various infectious risk factors, but there are few studies in the literature evaluating the actual impact of psoriasis in severe staphylococcal skin infections. Our narrative review of the literature suggests that psoriatic patients are at increased risk of both colonizati...on and severe infection, during hospitalization, by S. aureus. The latter also appears to play a role in the pathogenesis of psoriasis through the production of exotoxins. Hospitalized psoriatic patients are also at increased risk of MRSA skin infections. For this reason, new molecules are needed that could both overcome bacterial resistance and inhibit exotoxin production. In our opinion, in the near future, topical quorum sensing inhibitors in combination with current anti-MRSA therapies will be able to overcome the increasing resistance and block exotoxin production. Supplementation with Vitamin E (VE) or derivatives could also enhance the effect of anti-MRSA antibiotics, considering that psoriatic patients with metabolic comorbidities show a low intake of VE and low serum levels, making VE supplementation an interesting new perspective.
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In Vitro Activity of Novel Lipopeptides against Triazole-Resistant Aspergillus fumigatus
Anno: 2022
Rivista: JOURNAL OF FUNGI
Autori/autrici: Fioriti, Simona; Cirioni, Oscar; Simonetti, Oriana; Franca, Lucia; Candelaresi, Bianca; Pallotta, Francesco; Neubauer, Damian; Kamysz, Elzbieta; Kamysz, Wojciech; Canovari, Benedetta; Brescini, Lucia; Morroni, Gianluca; Barchiesi, Francesco
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/305719
Citazioni:
In Vitro Activity of Novel Lipopeptides against Triazole-Resistant Aspergillus fumigatus / Fioriti, S., Cirioni, O., Simonetti, O., Franca, L., Candelaresi, B., Pallotta, F., Neubauer, D., Kamysz, E., Kamysz, W., Canovari, B., Brescini, L., Morroni, G., Barchiesi, F.. - In: JOURNAL OF FUNGI. - ISSN 2309-608X. - (2022).

Clinical and microbiological features of ceftolozane/tazobactam-resistant Pseudomonas aeruginosa isolates in a university hospital in central Italy
Anno: 2022
Rivista: JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
Autori/autrici: Morroni, Gianluca; Brescini, Lucia; Antonelli, Alberto; Di Pilato, Vincenzo; Castelletti, Sefora; Brenciani, Andrea; D'Achille, Gloria; Mingoia, Marina; Giovanetti, Eleonora; Fioriti, Simona; Masucci, Annamaria; Giani, Tommaso; Giacometti, Andrea; Rossolini, GIAN MARIA; Cirioni, Oscar
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/305721
Citazioni:
Clinical and microbiological features of ceftolozane/tazobactam-resistant Pseudomonas aeruginosa isolates in a university hospital in central Italy / Morroni, G., Brescini, L., Antonelli, A., Di Pilato, V., Castelletti, S., Brenciani, A., D'Achille, G., Mingoia, M., Giovanetti, E., Fioriti, S., Masucci, A., Giani, T., Giacometti, A., Rossolini, G.M., Cirioni, O.. - In: JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE. - ISSN 2213-7165. - (2022).
Abstract:
Objectives: Ceftolozane/tazobactam (C/T) is a novel cephalosporin and β-lactamase inhibitor combination with great activity against Pseudomonas aeruginosa. To assess P. aeruginosa susceptibility to C/T, a surveil- lance study was conducted from October 2018 to March 2019 at the University Hos...pital ‘Ospedali Riuniti’ in Ancona, Italy. Methods: Minimum inhibitory concentrations (MICs) to C/T were determined by Etest strip. Resistant iso- lates were characterized by phenotypic (broth microdilution antimicrobial susceptibility testing and mod- ified Carbapenem Inactivation Method [mCIM]) and genotypic (Polymerase Chain Reaction [PCR], Pulsed Field Gel Electrophoresis [PFGE], and whole-genome sequencing [WGS]) methods. Clinical variables of patients infected by C/T-resistant P. aeruginosa were collected from medical records. Results: Fifteen of 317 P. aeruginosa collected showed resistance to C/T (4.7%). Ten strains demonstrated carbapenemase activity by mCIM method, and PCR confirmed that eight strains harbored a blaVIM gene while the other two were positive for blaIMP. Additionally, three isolates carried acquired extended spec- trum β-lactamase genes (two isolates carried blaPER and one carried blaGES). Eight strains were strictly related by PFGE and WGS analysis confirmed that they belonged to sequence type (ST)111. The other STs found were ST175 (two isolates), ST235 (two isolates), ST70 (one isolate), ST621 (one isolate), and the new ST3354 (one isolate). Most patients had received previous antibiotic therapies, carried invasive devices, and experienced prolonged hospitalization. Conclusion: This study demonstrated the presence of C/T-resistant P. aeruginosa isolates in a regional hospital carrying a number of resistance mechanisms acquired by different high-risk clones
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Role of Daptomycin in Cutaneous Wound Healing: A Narrative Review
Anno: 2022
Rivista: ANTIBIOTICS
Autori/autrici: Rizzetto, Giulio; Molinell, Elisa; Radi, Giulia; Diotallevi, Federico; Cirioni, Oscar; Brescini, Lucia; Giacometti, Andrea; Offidani, Annamaria; Simonetti, Oriana
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/305720
Citazioni:
Role of Daptomycin in Cutaneous Wound Healing: A Narrative Review / Rizzetto, G., Molinell, E., Radi, G., Diotallevi, F., Cirioni, O., Brescini, L., Giacometti, A., Offidani, A., Simonetti, O.. - In: ANTIBIOTICS. - ISSN 2079-6382. - (2022).
Abstract:
Daptomycin is active against Gram-positive bacteria, including methicillin-resistant Staphylococcus aureus (MRSA) and the on-label indications for its use include complicated skin and skin structure infections (cSSSI). We performed a narrative review of the literature with the aim to evaluate the ...role of daptomycin in the skin wound healing process, proposing our point of view on the possible association with other molecules that could improve the skin healing process. Daptomycin may improve wound healing in MRSA-infected burns, surgical wounds, and diabetic feet, but further studies in humans with histological examination are needed. In the future, the combination of daptomycin with other molecules with synergistic action, such as vitamin E and derivates, IB-367, RNA III-inhibiting peptide (RIP), and palladium nanoflowers, may help to improve wound healing and overcome forms of antibiotic resistance
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Methicillin-resistant Staphylococcus aureus as a cause of chronic wound infections: Alternative strategies for management
Anno: 2022
Rivista: AIMS MICROBIOLOGY
Autori/autrici: Simonetti, Oriana; Marasca, Samuele; Candelora, Matteo; Rizzetto, Giulio; Radi, Giulia; Molinelli, Elisa; Brescini, Lucia; Cirioni, Oscar; Offidani, Annamaria
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/305722
Citazioni:
Methicillin-resistant Staphylococcus aureus as a cause of chronic wound infections: Alternative strategies for management / Simonetti, O., Marasca, S., Candelora, M., Rizzetto, G., Radi, G., Molinelli, E., Brescini, L., Cirioni, O., Offidani, A.. - In: AIMS MICROBIOLOGY. - ISSN 2471-1888. - (2022).
Abstract:
Biofilm formation at the level of a wound plays an important role in its chronicization. The difficulty of its eradication has driven research toward the discovery and synthesis of new molecules that can act on biofilm to promote wound healing. This narrative review focuses on alternative molecules ...that can act and promote the eradication of methicillin-resistant Staphylococcus aureus, taking into consideration its antibiotic resistance, virulence, tendency toward the tenacious colonization of wounds by biofilms, and its increased prevalence in both community and hospital settings. A selection of promising studies were reported, analyzing the in vitro and/or in vivo efficacy of bacteriophages, metal nanoparticles, RNAIII inhibiting peptide (RIP), synthetized RIP derivatives, proteinase K and hamamelitannin.
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Role of daptomycin in cutaneous wound healing: a narrative review
Anno: 2022
Rivista: ANTIBIOTICS
Autori/autrici: Rizzetto, Giulio; Molinelli, Elisa; Radi, Giulia; Diotallevi, Federico; Cirioni, Oscar; Brescini, Lucia; Giacometti, Andrea; Offidani, Annamaria; Simonetti, Oriana
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/328493
Citazioni:
Role of daptomycin in cutaneous wound healing: a narrative review / Rizzetto, G., Molinelli, E., Radi, G., Diotallevi, F., Cirioni, O., Brescini, L., Giacometti, A., Offidani, A., Simonetti, O.. - In: ANTIBIOTICS. - ISSN 2079-6382. - 11:7(2022). [10.3390/antibiotics11070944]
Abstract:
Daptomycin is active against Gram-positive bacteria, including methicillin-resistant Staphylococcus aureus (MRSA) and the on-label indications for its use include complicated skin and skin structure infections (cSSSI). We performed a narrative review of the literature with the aim to evaluate the ro...le of daptomycin in the skin wound healing process, proposing our point of view on the possible association with other molecules that could improve the skin healing process. Daptomycin may improve wound healing in MRSA-infected burns, surgical wounds, and diabetic feet, but further studies in humans with histological examination are needed. In the future, the combination of daptomycin with other molecules with synergistic action, such as vitamin E and derivates, IB-367, RNA III-inhibiting peptide (RIP), and palladium nanoflowers, may help to improve wound healing and overcome forms of antibiotic resistance. © 2022 by the authors.
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The Effect of Dalbavancin in Moderate to Severe Hidradenitis Suppurativa
Anno: 2022
Rivista: ANTIBIOTICS
Autori/autrici: Molinelli, Elisa; Sapigni, Claudia; D'Agostino, Giovanni Marco; Brisigotti, Valerio; Rizzetto, Giulio; Bobyr, Ivan; Cirioni, Oscar; Giacometti, Andrea; Brescini, Lucia; Mazzanti, Sara; Offidani, Annamaria; Simonetti, Oriana
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/315732
Citazioni:
The Effect of Dalbavancin in Moderate to Severe Hidradenitis Suppurativa / Molinelli, E., Sapigni, C., D'Agostino, G.M., Brisigotti, V., Rizzetto, G., Bobyr, I., Cirioni, O., Giacometti, A., Brescini, L., Mazzanti, S., Offidani, A., Simonetti, O.. - In: ANTIBIOTICS. - ISSN 2079-6382. - ELETTRONICO. - 11:11(2022). [10.3390/antibiotics11111573]
Abstract:
Hidradenitis suppurativa (HS) is a chronic inflammatory skin disease characterized by painful nodules, abscesses, and fistulas, localized to the areas of the folds where apocrine glands are present: the armpits, groin, inframammary region, and genital or perineal region. The management is still chal...lenging, and it includes mainly systemic antibiotics, immunosuppressors, and biologic agents. Antibiotics are frequently used in the management of HS for their anti-inflammatory, immunomodulatory, and antimicrobial properties, but no data have been reported regarding the use of dalbavancin in HS. The aim of our practice was to evaluate efficacy, flare, and disease-free survival after dalbavancin therapy in a selected population with HS. We report the experience of the Ancona Dermatology Clinic in treating HS flare-ups with dalbavancin and its rationale for use. Our observation shows that the use of dalbavancin is an effective and well-tolerated treatment for the management of Hurley stage II-III HS; currently, dalbavancin should be considered as a supportive therapy for selected patients.
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Our Experience over 20 Years: Antimicrobial Peptides against Gram Positives, Gram Negatives, and Fungi
Anno: 2022
Rivista: PHARMACEUTICS
Autori/autrici: Rizzetto, Giulio; Gambini, Daisy; Maurizi, Andrea; Candelora, Matteo; Molinelli, Elisa; Cirioni, Oscar; Brescini, Lucia; Giacometti, Andrea; Offidani, Annamaria; Simonetti, Oriana
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/315728
Citazioni:
Our Experience over 20 Years: Antimicrobial Peptides against Gram Positives, Gram Negatives, and Fungi / Rizzetto, G., Gambini, D., Maurizi, A., Candelora, M., Molinelli, E., Cirioni, O., Brescini, L., Giacometti, A., Offidani, A., Simonetti, O.. - In: PHARMACEUTICS. - ISSN 1999-4923. - ELETTRONICO. - 15:1(2022). [10.3390/pharmaceutics15010040]
Abstract:
Antibiotic resistance is rapidly increasing, and new anti-infective therapies are urgently needed. In this regard, antimicrobial peptides (AMPs) may represent potential candidates for the treatment of infections caused by multiresistant microorganisms. In this narrative review, we reported the exper...ience of our research group over 20 years. We described the AMPs we evaluated against Gram-positive, Gram-negative, and fungi. In conclusion, our experience shows that AMPs can be a key option for treating multiresistant infections and overcoming resistance mechanisms. The combination of AMPs allows antibiotics and antifungals that are no longer effective to exploit the synergistic effect by restoring their efficacy. A current limitation includes poor data on human patients, the cost of some AMPs, and their safety, which is why studies on humans are needed as soon as possible.
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Ceftazidime-Avibactam for the Treatment of Multidrug-Resistant Pathogens: A Retrospective, Single Center Study
Anno: 2022
Rivista: ANTIBIOTICS
Autori/autrici: DI PIETRANTONIO, Maria; Brescini, Lucia; Candi, Jennifer; Morroni, Gianluca; Pallotta, Francesco; Mazzanti, Sara; Mantini, Paolo; Candelaresi, Bianca; Olivieri, Silvia; Ginevri, Francesco; Cesaretti, Giulia; Castelletti, Sefora; Cocci, Emanuele; Rosaria, G Polo; Cerutti, Elisabetta; Simonetti, Oriana; Cirioni, Oscar; Tavio, Marcello; Giacometti, Andrea; Barchiesi, Francesco
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/297812
Citazioni:
Ceftazidime-Avibactam for the Treatment of Multidrug-Resistant Pathogens: A Retrospective, Single Center Study / DI PIETRANTONIO, M., Brescini, L., Candi, J., Morroni, G., Pallotta, F., Mazzanti, S., Mantini, P., Candelaresi, B., Olivieri, S., Ginevri, F., Cesaretti, G., Castelletti, S., Cocci, E., Rosaria, G.P., Cerutti, E., Simonetti, O., Cirioni, O., Tavio, M., Giacometti, A., Barchiesi, F.. - In: ANTIBIOTICS. - ISSN 2079-6382. - 11:3(2022). [10.3390/antibiotics11030321]
Abstract:
Background: Ceftazidime/avibactam is a new cephalosporin/beta-lactamase inhibitor combination approved in 2015 by the FDA for the treatment of complicated intra-abdominal and urinary tract infection, hospital-acquired pneumoniae and Gram-negative infections with limited treatment options. Methods: I...n this retrospective study, we evaluate the efficacy of ceftazidime/avibactam treatment in 81 patients with Gram-negative infection treated in our center from January 2018 to December 2019. The outcome evaluated was 30-days survival or relapse of infection after the first positive blood culture. Results: the majority of patients were 56 male (69%), with median age of 67. Charlson’s Comorbidity Index was >3 in 58 patients. In total, 46% of the patients were admitted into the medical unit, 41% in the ICU, and 14% in the surgical ward. Of the patients, 78% had nosocomial infections, and 22% had healthcare-related infections. The clinical failure rate was 35%: 13 patients died within 30 days from the onset of infection. The outcome was influenced by the clinical condition of the patients: solid organ transplantation (p = 0.003) emerged as an independent predictor of mortality; non-survival patients most frequently had pneumonia (p = 0.009) or mechanical ventilation (p = 0.049). Conclusion: Ceftazidime–avibactam showed high efficacy in infections caused by MDR Gram-negative pathogens with limited therapeutic options.
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New insight into old and new antimicrobial molecules targeting quorum sensing for MRSA wound infection
Anno: 2022
Rivista: FUTURE MICROBIOLOGY
Autori/autrici: Simonetti, Oriana; Rizzetto, Giulio; Cirioni, Oscar; Molinelli, Elisa; Morroni, Gianluca; Giacometti, Andrea; Offidani, Annamaria
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/306467
Citazioni:
New insight into old and new antimicrobial molecules targeting quorum sensing for MRSA wound infection / Simonetti, O., Rizzetto, G., Cirioni, O., Molinelli, E., Morroni, G., Giacometti, A., Offidani, A.. - In: FUTURE MICROBIOLOGY. - ISSN 1746-0913. - 17:3(2022), pp. 177-183. [10.2217/fmb-2021-0120]
Abstract:
MRSA represents one of the largest problems in wound healing as a result of its increasing incidence and the complex therapeutic approach required to treat it. The need for new solutions to overcome antibiotic resistance led to the development of antimicrobial molecules that are effective at blockin...g quorum sensing. This special report provides an up-to-date review, based on the latest evidence in the literature, of old and new molecules that can positively influence the process of wound healing via their action on MRSA quorum sensing. Quorum sensing-inhibiting molecules, applied topically or injected in situ, have excellent potential to improve both MRSA eradication and quality of wound healing, especially when combined with conventional systemic MRSA therapy. Further human studies are needed to evaluate the efficacy of these molecules.
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Efficacy of cathelicidin ll-37 in an mrsa wound infection mouse model
Anno: 2021
Rivista: ANTIBIOTICS
Autori/autrici: Simonetti, O.; Cirioni, O.; Goteri, G.; Lucarini, G.; Kamysz, E.; Kamysz, W.; Orlando, F.; Rizzetto, G.; Molinelli, E.; Morroni, G.; Ghiselli, R.; Provinciali, M.; Giacometti, A.; Offidani, A.
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/297885
Citazioni:
Efficacy of cathelicidin ll-37 in an mrsa wound infection mouse model / Simonetti, O., Cirioni, O., Goteri, G., Lucarini, G., Kamysz, E., Kamysz, W., Orlando, F., Rizzetto, G., Molinelli, E., Morroni, G., Ghiselli, R., Provinciali, M., Giacometti, A., Offidani, A.. - In: ANTIBIOTICS. - ISSN 2079-6382. - 10:10(2021). [10.3390/antibiotics10101210]
Abstract:
Background: LL-37 is the only human antimicrobial peptide that belongs to the cathelici-dins. The aim of the study was to evaluate the efficacy of LL-37 in the management of MRSA-in-fected surgical wounds in mice. Methods: A wound on the back of adult male BALB/c mice was made and inoculated with St...aphylococcus aureus. Two control groups were formed (uninfected and not treated, C0; infected and not treated, C1) and six contaminated groups were treated, respec-tively, with: teicoplanin, LL-37, given topically and /or systemically. Histological examination of VEGF expression and micro-vessel density, and bacterial cultures of wound tissues, were per-formed. Results: Histological examination of wounds in the group treated with topical and intra-peritoneal LL-37 showed increased re-epithelialization, formation of the granulation tissue, collagen organization, and angiogenesis. Conclusions: Based on the mode of action, LL-37 has a potential future role in the management of infected wounds.
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Safety and efficacy of vaccines during covid-19 pandemic in patients treated with biological drugs in a dermatological setting
Anno: 2021
Rivista: HEALTHCARE
Autori/autrici: Simonetti, O.; Rizzetto, G.; Molinelli, E.; Diotallevi, F.; Radi, G.; Cirioni, O.; D'Errico, M. M.; Offidani, A.
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/300224
Citazioni:
Safety and efficacy of vaccines during covid-19 pandemic in patients treated with biological drugs in a dermatological setting / Simonetti, O., Rizzetto, G., Molinelli, E., Diotallevi, F., Radi, G., Cirioni, O., D'Errico, M.M., Offidani, A.. - In: HEALTHCARE. - ISSN 2227-9032. - 9:4(2021). [10.3390/healthcare9040401]
Abstract:
The BNT162b2 and mRNA-1273 vaccines, consisting of mRNA, have recently become available. The absolute novelty of these vaccines introduces questions about their safety and efficacy, especially in patients who are treated with biological drugs in dermatology. The aim of our review was to provide a br...oad overview of the current use of all available vaccinations in concomitance with biological therapy and to suggest indications for the new mRNA Covid-19 vaccines. We conducted a narrative review of the literature regarding the indications and safety of the various types of vaccines currently available in dermatological patients treated with biological therapy. The safety and efficacy of administering inactivated vaccines in patients undergoing biological therapy with inhibitors of TNF-α, IL-17, IL-12/23, and IL-4/13 was confirmed. Inactivated vaccines can be administered during therapy with inhibitors of IL-23 and IgE, taking into account that the level of evidence is lower due to the lack of specific studies. Live attenuated vaccines were contraindicated in concomitance with all biological therapies considered, except omalizumab. According to this evidence, we assume that there are currently no contraindications to the administration of the new Covid-19 BNT162b2 and mRNA-1273 vaccines during biological therapy with inhibitors of TNF-α, IL-17, IL-12/23, IL-23, and IL-4/13, since these vaccines are comparable to inactivated ones. For patients with chronic urticaria or allergic asthma treated with omalizumab, we currently recommend caution in using the mRNA Covid-19 vaccines (30 min observation). The only contraindications were a previous history of hypersensitivity to the Covid-19 vaccines themself or to their excipients. In conclusion, further randomized clinical trials are needed to evaluate the efficacy of the antibody response in these patients.
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Review: A safety profile of dalbavancin for onand off-label utilization
Anno: 2021
Rivista: THERAPEUTICS AND CLINICAL RISK MANAGEMENT
Autori/autrici: Simonetti, O.; Rizzetto, G.; Molinelli, E.; Cirioni, O.; Offidani, A.
Lingua: ENG
Classificazione IRIS: 1.2 Recensione in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/290201
Citazioni:
Review: A safety profile of dalbavancin for onand off-label utilization / Simonetti, O., Rizzetto, G., Molinelli, E., Cirioni, O., Offidani, A.. - In: THERAPEUTICS AND CLINICAL RISK MANAGEMENT. - ISSN 1176-6336. - ELETTRONICO. - 17:(2021), pp. 223-232. [10.2147/TCRM.S271445]
Abstract:
Introduction: Dalbavancin is a bactericidal lipoglycopeptide active against gram-positives. Its use has been approved for the treatment of acute bacterial skin and skin structure infections (ABSSSI). Methods: We conducted a narrative review of the literature on the safety profile of dalbavancin. The... bibliographic research was carried out on the PubMed database on 6 November 2020 by seeking combinations of the following keywords: Dalbavancin, adverse effects, safety, drug interactions, and skin infections. Results: Five double-blind Phase 3 randomized clinical trials, 2 open-label randomized trials, and 4 retrospective studies were identified. No statistically significant differences were found between dalbavancin and comparators in the incidence of adverse events. Retrospective studies confirm the low incidence of adverse events. Conclusion: Dalbavancin is a therapeutic option that has demonstrated an excellent safety profile, also in relation to the other MRSA therapies available. Its use represents a costeffective solution for the treatment of those patients with ABSSSI who would need hospitalization. One limitation of this study is that most of the available data are from Phase III clinical trials. Further real-life studies with a larger sample size are therefore needed to better assess the safety profile of the dalbavancin, especially to investigate the true incidence of rare adverse events.
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New perspectives on old and new therapies of staphylococcal skin infections: the role of biofilm targeting in wound healing.
Anno: 2021
Rivista: ANTIBIOTICS
Autori/autrici: Simonetti, O; Rizzetto, G; Radi, G; Molinelli, E; Cirioni, O; Giacometti, A; Offidani, Annamaria
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/300240
Citazioni:
New perspectives on old and new therapies of staphylococcal skin infections: the role of biofilm targeting in wound healing / Simonetti, O., Rizzetto, G., Radi, G., Molinelli, E., Cirioni, O., Giacometti, A., Offidani, A.. - In: ANTIBIOTICS. - ISSN 2079-6382. - STAMPA. - 10:11(2021). [10.3390/antibiotics10111377]
Abstract:
Among the most common complications of both chronic wound and surgical sites are staphylococcal skin infections, which slow down the wound healing process due to various virulence factors, including the ability to produce biofilms. Furthermore, staphylococcal skin infections are often caused by meth...icillin-resistant Staphylococcus aureus (MRSA) and become a therapeutic challenge. The aim of this narrative review is to collect the latest evidence on old and new anti-staphylococcal therapies, assessing their anti-biofilm properties and their effect on skin wound healing. We considered antibiotics, quorum sensing inhibitors, antimicrobial peptides, topical dressings, and antimicrobial photo-dynamic therapy. According to our review of the literature, targeting of biofilm is an important therapeutic choice in acute and chronic infected skin wounds both to overcome antibiotic resistance and to achieve better wound healing.
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Use of dalbavancin in skin, bone and joint infections: A real-life experience in an Italian center
Anno: 2021
Rivista: ANTIBIOTICS
Autori/autrici: Brescini, L.; Martera, F. D.; Morroni, G.; Mazzanti, S.; Di Pietrantonio, M.; Mantini, P.; Candelaresi, B.; Pallotta, F.; Olivieri, S.; Iencinella, V.; Castelletti, S.; Cocci, E.; Polo, R. G.; Veccia, S.; Cirioni, O.; Tavio, M.; Giacometti, A.
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/294435
Citazioni:
Use of dalbavancin in skin, bone and joint infections: A real-life experience in an Italian center / Brescini, L., Martera, F.D., Morroni, G., Mazzanti, S., Di Pietrantonio, M., Mantini, P., Candelaresi, B., Pallotta, F., Olivieri, S., Iencinella, V., Castelletti, S., Cocci, E., Polo, R.G., Veccia, S., Cirioni, O., Tavio, M., Giacometti, A.. - In: ANTIBIOTICS. - ISSN 2079-6382. - 10:9(2021). [10.3390/antibiotics10091129]
Abstract:
Dalbavancin is a lipoglycopeptide approved for the treatment of acute bacterial skin and skin structure infections (ABSSSI). The aim of the study was to evaluate the efficacy and safety in all patients who received at least one administration of dalbavancin. Methods: We carried out a retrospective s...tudy of the use of dalbavancin in 55 patients at the Azienda Ospedaliera Ospedali Riuniti Umberto I (Ancona, Italy) from February 2017 to May 2020 and compared “on label” and “off label” use of dalbavancin in ABSSSI and non-ABSSSI. Results: A total of 55 patients were included in the study. The median age was 61 years; 51% had ABSSSI; 24% had prosthetic joint infections, and 14% had osteomyelitis. A total of 53% received a single 1500 mg infusion of dalbavancin, and 18% received a second dose 14 days later; 24% of patients received further doses at 14-day intervals. In 91% of cases, patients achieved clinical objectives with dalbavancin: 96% of patients with ABSSSI and 69% of those with prosthetic joint infections. Conclusions: Dalbavancin was shown to have an excellent tolerability profile and to be a highly successful therapeutic approach even in those cases treated “off-label”.
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Vaccines for COVID-19 in patients with atopic dermatitis: Three things every dermatologist should know
Anno: 2021
Rivista: ACTA DERMATOVENEROLOGICA ALPINA, PANONICA, ET ADRIATICA
Autori/autrici: Diotallevi, F.; Simonetti, O.; Radi, G.; Molinelli, E.; Rizzetto, G.; Cirioni, O.; D'Errico, M. M.; Offidani, A.
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/300225
Citazioni:
Vaccines for COVID-19 in patients with atopic dermatitis: Three things every dermatologist should know / Diotallevi, F., Simonetti, O., Radi, G., Molinelli, E., Rizzetto, G., Cirioni, O., D'Errico, M.M., Offidani, A.. - In: ACTA DERMATOVENEROLOGICA ALPINA, PANONICA, ET ADRIATICA. - ISSN 1318-4458. - STAMPA. - 30:2(2021), pp. 67-69. [10.15570/actaapa.2021.16]
Abstract:
Almost 13 months have passed since the World Health Organization (WHO) declared the coronavirus disease 19 (COVID-19) pandemic, caused by the SARS-CoV-2, on March 11th, 2020. During this period, we have realized that the most effective weapon we have to prevent SARS-CoV-2 infection, or to make it le...ss aggressive, is vaccines. Currently, according to the WHO document “Draft landscape of COVID-19 candidate vaccines,” there are 275 vaccines in development against the virus, although at the moment there are four preparations in distribution in the United States and in Europe. The characteristics of these vaccines are quite different from each other and may even be unfamiliar in the medical field. In particular, among dermatologists, knowledge of vaccines is of fundamental importance, especially in atopic dermatitis. Atopic patients are aware of having a predisposition to develop allergies, and so they are asking dermatologists about the safety of the vaccines currently available against the SARS-CoV-2. This article provides an up-to-date overview of this topic by reviewing current literature and sharing our personal experience.
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Synergistic effect of antimicrobial peptide LL-37 and colistin combination against multidrug-resistant Escherichia coli isolates
Anno: 2021
Rivista: FUTURE MICROBIOLOGY
Autori/autrici: Morroni, Gianluca; Sante, Laura Di; Simonetti, Oriana; Brescini, Lucia; Kamysz, Wojciech; Kamysz, Elzbieta; Mingoia, Marina; Brenciani, Andrea; Giovanetti, Eleonora; Bagnarelli, Patrizia; Giacometti, Andrea; Cirioni, Oscar
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/288297
Citazioni:
Synergistic effect of antimicrobial peptide LL-37 and colistin combination against multidrug-resistant Escherichia coli isolates / Morroni, G., Sante, L.D., Simonetti, O., Brescini, L., Kamysz, W., Kamysz, E., Mingoia, M., Brenciani, A., Giovanetti, E., Bagnarelli, P., Giacometti, A., Cirioni, O.. - In: FUTURE MICROBIOLOGY. - ISSN 1746-0913. - (2021). [10.2217/fmb-2020-0204]
Abstract:
Overview: The global spread of antibiotic resistance represents a serious threat for public health. Aim: We evaluated the efficacy of the antimicrobial peptide LL-37 as antimicrobial agent against multidrug-resistant Escherichia coli. Results: LL-37 showed good activity against mcr-1 carrying, exten...ded spectrum beta-lactamase- and carbapenemase-producing E. coli (minimum inhibitory concentration, MIC, from 16 to 64mg/l). Checkerboard assays demonstrated synergistic effect of LL-37/colistin combination against all tested strains, further confirmed by time-kill and post antibiotic effect assays. MIC and sub-MIC concentrations of LL-37 were able to reduce biofilm formation. Conclusion: Our preliminary data indicated that LL-37/colistin combination was effective against multidrug resistant E. coli strains and suggested a new possible clinical application.
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Antimicrobial activity of aztreonam in combination with old and new β-lactamase inhibitors against mbl and esbl co-producing gram-negative clinical isolates: Possible options for the treatment of complicated infections
Anno: 2021
Rivista: ANTIBIOTICS
Autori/autrici: Morroni, G.; Bressan, R.; Fioriti, S.; D'Achille, G.; Mingoia, M.; Cirioni, O.; Di Bella, S.; Piazza, A.; Comandatore, F.; Mauri, C.; Migliavacca, R.; Luzzaro, F.; Principe, L.; Lagatolla, C.
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/294433
Citazioni:
Antimicrobial activity of aztreonam in combination with old and new β-lactamase inhibitors against mbl and esbl co-producing gram-negative clinical isolates: Possible options for the treatment of complicated infections / Morroni, G., Bressan, R., Fioriti, S., D'Achille, G., Mingoia, M., Cirioni, O., Di Bella, S., Piazza, A., Comandatore, F., Mauri, C., Migliavacca, R., Luzzaro, F., Principe, L., Lagatolla, C.. - In: ANTIBIOTICS. - ISSN 2079-6382. - 10:11(2021). [10.3390/antibiotics10111341]
Abstract:
Metallo-β-lactamases (MBLs) are among the most challenging bacterial enzymes to over-come. Aztreonam (ATM) is the only β-lactam not hydrolyzed by MBLs but is often inactivated by co-produced extended-spectrum β-lactamases (ESBL). We assessed the activity of the combination of ATM with... old and new β-lactamases inhibitors (BLIs) against MBL and ESBL co-producing Gram-negative clinical isolates. Six Enterobacterales and three non-fermenting bacilli co-producing MBL and ESBL determinants were selected as difficult-to-treat pathogens. ESBLs and MBLs genes were characterized by PCR and sequencing. The activity of ATM in combination with seven different BLIs (clavulanate, sulbactam, tazobactam, vaborbactam, avibactam, relebactam, zidebactam) was assessed by microdilution assay and time–kill curve. ATM plus avibactam was the most effective combination, able to restore ATM susceptibility in four out of nine tested isolates, reaching in some cases a 128-fold reduction of the MIC of ATM. In addition, relebactam and zidebactam showed to be effective, but with lesser reduction of the MIC of ATM. E. meningoseptica and C. indologenes were not inhibited by any ATM–BLI combination. ATM–BLI combinations demonstrated to be promising against MBL and ESBL co-producers, hence providing multiple options for treatment of related infections. However, no effective combination was found for some non-fermentative bacilli, suggesting the presence of additional resistance mechanisms that complicate the choice of an active therapy.
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Characterization and clonal diffusion of ceftaroline non-susceptible mrsa in two hospitals in central Italy
Anno: 2021
Rivista: ANTIBIOTICS
Autori/autrici: Morroni, G.; Fioriti, S.; Salari, F.; Brenciani, A.; Brescini, L.; Mingoia, M.; Giovanetti, E.; Pocognoli, A.; Giacometti, A.; Molinelli, E.; Offidani, A.; Simonetti, O.; Cirioni, O.
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/293309
Citazioni:
Characterization and clonal diffusion of ceftaroline non-susceptible mrsa in two hospitals in central Italy / Morroni, G., Fioriti, S., Salari, F., Brenciani, A., Brescini, L., Mingoia, M., Giovanetti, E., Pocognoli, A., Giacometti, A., Molinelli, E., Offidani, A., Simonetti, O., Cirioni, O.. - In: ANTIBIOTICS. - ISSN 2079-6382. - 10:8(2021). [10.3390/antibiotics10081026]
Abstract:
Background: Ceftaroline represents a novel fifth-generation cephalosporin to treat infections caused by methicillin-resistant Staphylococcus aureus (MRSA). Methods: Ceftaroline susceptibility of 239 MRSA isolates was assessed by disk diffusion and a MIC test strip following both EUCAST and CLSI guid...elines. Non-susceptible isolates were epidemiologically characterized by pulsed-field gel electrophoresis, spa typing, and multilocus sequence typing, and further investigated by PCR and whole genome sequencing to detect penicillin-binding protein (PBP) mutations as well as antibiotic resistance and virulence genes. Results: Fourteen isolates out of two hundred and thirty-nine (5.8%) were non-susceptible to ceftaroline (MIC > 1 mg/L), with differences between the EUCAST and CLSI interpretations. The characterized isolates belonged to seven different pulsotypes and three different clones (ST228/CC5-t041-SCCmecI, ST22/CC22-t18014-SCCmecIV, and ST22/CC22-t022-SCCmecIV), confirming a clonal diffusion of ceftaroline non-susceptible strains. Mutations in PBPs involved PBP2a for ST228-t041-SCCmecI strains and all the other PBPs for ST22-t18014-SCCmecIV and ST22-t022-SCCmecIV clones. All isolates harbored antibiotic resistance and virulence genes with a clonal distribution. Conclusion: Our study demonstrated that ceftaroline non-susceptibile isolates belonged not only to ST228 strains (the most widespread clone in Italy) but also to ST22, confirming the increasing role of these clones in hospital infections.
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Witch hazel significantly improves the efficacy of commercially available teat dips
Anno: 2020
Rivista: PATHOGENS
Autori/autrici: Rasooly, R.; Molnar, A.; Do, P.; Morroni, G.; Brescini, L.; Cirioni, O.; Giacometti, A.; Apostolidis, E.
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/276806
Citazioni:
Witch hazel significantly improves the efficacy of commercially available teat dips / Rasooly, R., Molnar, A., Do, P., Morroni, G., Brescini, L., Cirioni, O., Giacometti, A., Apostolidis, E.. - In: PATHOGENS. - ISSN 2076-0817. - 9:2(2020), p. 92. [10.3390/pathogens9020092]
Abstract:
Bovine intramammary infections (IMIs) are the main cause of economic loss in milk production. Antibiotics are often ineffective in treating infections due to antimicrobial resistance and the formation of bacterial biofilms that enhance bacterial survival and persistence. Teat dips containing germici...des are recommended to prevent new IMIs and improve udder health and milk quality. IMIs are often caused by staphylococci, which are Gram-positive bacteria that become pathogenic by forming biofilms and producing toxins. As a model for a teat dip (DIP), the BacStop iodine-based teat dip (DIP) was used. Witch hazel extract (whISOBAX (WH)) was tested because it contains a high concentration of the anti-biofilm/anti-toxin phenolic compound hamamelitannin. We found that the minimal inhibitory or bactericidal concentrations of DIP against planktonic S. epidermidis cells increased up to 160-fold in the presence of WH, and that DIP was 10-fold less effective against biofilm cells. While both DIP and WH are effective in inhibiting the growth of S. aureus, only WH inhibits toxin production (tested for enterotoxin-A). Importantly, WH also significantly enhances the antibacterial effect of DIP against Gram-negative bacteria that can cause IMIs, like Escherichia coli and Pseudomonas aeruginosa. Put together, these results suggest that the antibacterial activity of DIP combined with WH is significantly higher, and thus have potential in eradicating bacterial infections, both in acute (planktonic-associated) and in chronic (biofilm-associated) conditions.
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whISOBAX™ inhibits bacterial pathogenesis and enhances the effect of antibiotics
Anno: 2020
Rivista: ANTIBIOTICS
Autori/autrici: Rasooly, R.; Choi, H. -Y.; Do, P.; Morroni, G.; Brescini, L.; Cirioni, O.; Giacometti, A.; Apostolidis, E.
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/282349
Citazioni:
whISOBAX™ inhibits bacterial pathogenesis and enhances the effect of antibiotics / Rasooly, R., Choi, H.-Y., Do, P., Morroni, G., Brescini, L., Cirioni, O., Giacometti, A., Apostolidis, E.. - In: ANTIBIOTICS. - ISSN 2079-6382. - 9:5(2020), p. 264. [10.3390/antibiotics9050264]
Abstract:
As bacteria are becoming more resistant to commonly used antibiotics, alternative therapies are being sought. whISOBAX (WH) is a witch hazel extract that is highly stable (tested up to 2 months in 37◦ C) and contains a high phenolic content, where 75% of it is hamamelitannin and traces of gallic a...cid. Phenolic compounds like gallic acid are known to inhibit bacterial growth, while hamamelitannin is known to inhibit staphylococcal pathogenesis (biofilm formation and toxin production). WH was tested in vitro for its antibacterial activity against clinically relevant Gram-positive and Gram-negative bacteria, and its synergy with antibiotics determined using checkerboard assays followed by isobologram analysis. WH was also tested for its ability to suppress staphylococcal pathogenesis, which is the cause of a myriad of resistant infections. Here we show that WH inhibits the growth of all bacteria tested, with variable efficacy levels. The most WH-sensitive bacteria tested were Staphylococcus epidermidis, Staphylococcus aureus, Enterococcus faecium and Enterococcus faecalis, followed by Acinetobacter baumannii, Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa, Streptococcus agalactiae and Streptococcus pneumoniae. Furthermore, WH was shown on S. aureus to be synergistic to linezolid and chloramphenicol and cumulative to vancomycin and amikacin. The effect of WH was tested on staphylococcal pathogenesis and shown here to inhibit biofilm formation (tested on S. epidermidis) and toxin production (tested on S. aureus Enterotoxin A (SEA)). Toxin inhibition was also evident in the presence of subinhibitory concentrations of ciprofloxacin that induces pathogenesis. Put together, our study indicates that WH is very effective in inhibiting the growth of multiple types of bacteria, is synergistic to antibiotics, and is also effective against staphylococcal pathogenesis, often the cause of persistent infections. Our study thus suggests the benefits of using WH to combat various types of bacterial infections, especially those that involve resistant persistent bacterial pathogens.
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Viscoelastic behaviour of hyaluronic acid formulations containing carvacrol prodrugs with antibacterial properties
Anno: 2020
Rivista: INTERNATIONAL JOURNAL OF PHARMACEUTICS
Autori/autrici: Marinelli, L.; Cacciatore, I.; Eusepi, P.; Di Biase, G.; Morroni, G.; Cirioni, O.; Giacometti, A.; Di Stefano, A.
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/282341
Citazioni:
Viscoelastic behaviour of hyaluronic acid formulations containing carvacrol prodrugs with antibacterial properties / Marinelli, L., Cacciatore, I., Eusepi, P., Di Biase, G., Morroni, G., Cirioni, O., Giacometti, A., Di Stefano, A.. - In: INTERNATIONAL JOURNAL OF PHARMACEUTICS. - ISSN 0378-5173. - 582:(2020), p. 119306. [10.1016/j.ijpharm.2020.119306]
Abstract:
In this paper, we report the development and viscoelastic properties of hyaluronic acid formulations (HA5, HA30, and HA60, containing 0.5, 3, and 6% HA, respectively) loaded with carvacrol prodrugs (WSCPS) with antibacterial properties. Notably, antimicrobial studies revealed that WSCP1-2 in both HA...5 and HA30 formulations showed the best minimum inhibitory concentration (MIC) values against Enterococcus faecium (128 mg/L) and Enterococcus faecalis (256 mg/L) compared to those of carvacrol alone or in formulations with HA. Moreover, rheological analyses showed that HA30 composites exhibited a semi-solid consistency, while HA5 formulations possessed a fluid consistency. Considering these data, HA30 is a useful formulation which guarantees a good percentage of prodrug release (e.g., 30 and 60% for WSCP1 and 2, respectively) as well as a texture suitable for topical administration to treat wounds and/or skin infections.
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Trend of clinical vancomycin-resistant enterococci isolated in a regional Italian hospital from 2001 to 2018
Anno: 2020
Rivista: BRAZILIAN JOURNAL OF MICROBIOLOGY
Autori/autrici: Fioriti, S.; Simoni, S.; Caucci, S.; Morroni, G.; Ponzio, E.; Coccitto, S. N.; Brescini, L.; Cirioni, O.; Menzo, S.; Biavasco, F.; Giovanetti, E.; Brenciani, A.; Vignaroli, C.
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/283629
Citazioni:
Trend of clinical vancomycin-resistant enterococci isolated in a regional Italian hospital from 2001 to 2018 / Fioriti, S., Simoni, S., Caucci, S., Morroni, G., Ponzio, E., Coccitto, S.N., Brescini, L., Cirioni, O., Menzo, S., Biavasco, F., Giovanetti, E., Brenciani, A., Vignaroli, C.. - In: BRAZILIAN JOURNAL OF MICROBIOLOGY. - ISSN 1517-8382. - (2020). [10.1007/s42770-020-00360-8]
Abstract:
A retrospective study of the epidemiology of vancomycin-resistant enterococci (VRE) in a regional hospital of central Italy in 2001–2018 demonstrated an increased VRE prevalence since 2016. A total of 113 VRE isolates, 89 E. faecium (VREfm) and 24 E. faecalis (VREfs), were collected in the stu...dy period. All strains showed high-level resistance to vancomycin; 107 also showed teicoplanin resistance. Altogether, 84 VREfm and 20 VREfs carried vanA, whereas 5 VREfm and 1 VREfs carried vanB. MLST analysis documented that 89 VREfm isolates mainly belonged to ST78, ST80, and ST117. Most strains were isolated from 2001 to 2007, ST78 being the predominant clone. VREfm re-emerged in 2016 with a prevalence of the ST80 lineage. Most VREfs were isolated from 2001 to 2006; although they belonged to 7 different STs, there was a prevalence of ST88 and ST6. Notably, ST88 was sporadically recovered throughout the study period. The increasing rate of VREfm isolation from 2016 to 2018 may be related to the influx of new successful clones and to the renewed and widespread use of vancomycin. Improved infection control measures in hospital wards should be adopted to limit the spread of new epidemic VRE strains.
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New evidence and insights on dalbavancin and wound healing in a mouse model of skin infection
Anno: 2020
Rivista: ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
Autori/autrici: Simonetti, O.; Lucarini, G.; Morroni, G.; Orlando, F.; Lazzarini, R.; Zizzi, A.; Brescini, L.; Provinciali, M.; Giacometti, A.; Offidani, A.; Cirioni, O.
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/276659
Citazioni:
New evidence and insights on dalbavancin and wound healing in a mouse model of skin infection / Simonetti, O., Lucarini, G., Morroni, G., Orlando, F., Lazzarini, R., Zizzi, A., Brescini, L., Provinciali, M., Giacometti, A., Offidani, A., Cirioni, O.. - In: ANTIMICROBIAL AGENTS AND CHEMOTHERAPY. - ISSN 0066-4804. - STAMPA. - 64:4(2020). [10.1128/AAC.02062-19]
Abstract:
Dalbavancin is an effective antibiotic that is widely used to treat skin infection. Our aim was to determine the effect of dalbavancin administration on wound healing compared to that of vancomycin and to elucidate if epidermal growth factor receptor (EGFR), matrix metalloproteinase 1 (MMP-1), MMP-9..., and vascular endothelial growth factor (VEGF) could be involved in its therapeutic mechanism. A mouse model of methicillin-resistant Staphylococcus aureus (MRSA) skin infection was established. Mice were treated daily with vancomycin (10 mg/kg) and weekly with dalbavancin at day 1 (20 mg/kg) and day 8 (10 mg/kg). After 14 days, wounds were excised, and bacterial counts were performed. Wound healing was assessed by histological and immunohistochemical staining, followed by protein extraction and immunoblotting. Our microbiological results confirmed that both dalbavancin and vancomycin are effective in reducing the bacterial load in wounds. The dalbavancin group showed a strong effect compared with infected untreated animals and the vancomycin-treated group. The wounds treated with dalbavancin showed robust epidermal coverage with reconstitution of the regular and keratinized epidermal lining and well-organized granulation tissue with numerous blood vessels, although slightly less than that in the uninfected group. While in the vancomycin-treated group the epithelium appeared, in general, still hypertrophic, the granulation tissue appeared even less organized. We observed elevated EGFR and VEGF expression in both treated groups, although it was higher in dalbavancin-treated mice. MMP-1 and MMP-9 were decreased in uninfected tissue and in both treated tissues compared with untreated infected wounds. This study showed faster healing with dalbavancin treatment that might be associated with higher EGFR and VEGF levels.
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Antimicrobial Resistance: A Challenge for the Future
Anno: 2020
Autori/autrici: Varaldo, Pietro Emanuele; Facinelli, Bruna; Bagnarelli, Patrizia; Menzo, Stefano; Mingoia, Marina; Brenciani, Andrea; Giacometti, Andrea; Barchiesi, Francesco; Brescini, Lucia; Cirioni, Oscar; Scalise, Giorgio; Barbadoro, Pamela; Di Stanislao, Francesco; Prospero, Emilia; D'Errico, Marcello Mario
Lingua: ENG
Classificazione IRIS: 2.1 Contributo in volume (Capitolo o Saggio)
Scheda completa IRIS: https://iris.univpm.it/handle/11566/351592
Citazioni:
Antimicrobial Resistance: A Challenge for the Future / Varaldo, P.E., Facinelli, B., Bagnarelli, P., Menzo, S., Mingoia, M., Brenciani, A., Giacometti, A., Barchiesi, F., Brescini, L., Cirioni, O., Scalise, G., Barbadoro, P., Di Stanislao, F., Prospero, E., D'Errico, M.M.. - STAMPA. - (2020), pp. 13-29. [10.1007/978-3-030-33832-9_2]
Abstract:
The global emergence of antibiotic-resistance, together with the lack of/reduced development of new antibiotic molecules, currently represents a serious public health problem as it can mean the return to a pre-antibiotic era in which infections caused by multiple-resistant pathogens are intractable.... Since the beginnings, the interest of the Institutes of Microbiology, Hygiene and Public Health, and Infectious Diseases was focused on antibiotic resistance: from molecular mechanisms, through epidemiology and clinical issues, to prevention. Future perspectives include the search of new strategies and/or new compounds for prevention and control of difficult-to-treat pathogens in a multidisciplinary approach.
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Characterization of a new transferable MDR plasmid carrying the pbp5 gene from a clade B commensal Enterococcus faecium
Anno: 2019
Rivista: JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
Autori/autrici: Morroni, Gianluca; Brenciani, Andrea; Litta-Mulondo, Alice; Vignaroli, Carla; Mangiaterra, Gianmarco; Fioriti, Simona; Citterio, Barbara; Cirioni, Oscar; Giovanetti, Eleonora; Biavasco, Francesca
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/262816
Citazioni:
Characterization of a new transferable MDR plasmid carrying the pbp5 gene from a clade B commensal Enterococcus faecium / Morroni, G., Brenciani, A., Litta-Mulondo, A., Vignaroli, C., Mangiaterra, G., Fioriti, S., Citterio, B., Cirioni, O., Giovanetti, E., Biavasco, F.. - In: JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY. - ISSN 0305-7453. - ELETTRONICO. - 74:4(2019), pp. 843-850. [10.1093/jac/dky549]
Abstract:
OBJECTIVES: To evaluate the transferability of antibiotic resistance from an MDR clade B Enterococcus faecium and to characterize the genetic elements involved. METHODS: The erm(B)-positive strain E. faecium 37BA (donor) and strains E. faecium 64/3 and Listeria welshimeri 11857RF (recipients) w...ere used in mating experiments. Donors and transconjugants were characterized using MIC assays, PFGE, Southern blotting and hybridization, quantitative RT-PCR (RT-qPCR), next-generation sequencing and PCR mapping. RESULTS: One E. faecium and one L. welshimeri transconjugant were selected for in-depth investigation. Both acquired an ∼40 kb plasmid carrying erm(B). An additional plasmid of ∼200 kb, encoding the full conjugation machinery, was detected in the donor and in the E. faecium transconjugant. Next-generation sequencing revealed a new 40 396 bp plasmid that was designated pEf37BA; it contained 10 antibiotic resistance genes, tet(M), tet(L), erm(B), aadE, sat4, aphA, spw, lsa(E), lnu(B) and pbp5, resulting from the recombination of pM7M2 of E. faecium with an MDR chromosomal region of Erysipelothrix rhusiopathiae. A pbp5-carrying circular form was also detected. The PBP5 amino acid sequence differed from the C46 variant by two mutations (S39T and D644N). Its expression was documented in both transconjugants. pEf37BA persisted in the absence of selective pressure. CONCLUSIONS: The MDR clade B E. faecium plasmid, deriving from the recombination of two different resistance regions, carried a pbp5 element and was transferable to different bacterial species. This finding further documents the dissemination of ampicillin resistance among community-associated E. faecium and the key role of commensal strains in the spread of antibiotic resistance.
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Synergic combinations of antimicrobial peptides (AMPs) against biofilms of methicillin-resistant Staphylococcus aureus (MRSA) on polystyrene and medical devices
Anno: 2019
Rivista: JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
Autori/autrici: Ciandrini, Eleonora; Morroni, Gianluca; Cirioni, Oscar; Kamysz, Wojciech; Kamysz, Elzbieta; Brescini, Lucia; Baffone, Wally; Campana, Raffaella
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/276807
Citazioni:
Synergic combinations of antimicrobial peptides (AMPs) against biofilms of methicillin-resistant Staphylococcus aureus (MRSA) on polystyrene and medical devices / Ciandrini, E., Morroni, G., Cirioni, O., Kamysz, W., Kamysz, E., Brescini, L., Baffone, W., Campana, R.. - In: JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE. - ISSN 2213-7165. - (2019). [10.1016/j.jgar.2019.10.022]
Abstract:
Antimicrobial research is being focused to look for more effective therapeutics against antibiotic-resistant infections caused by methicillin-resistantStaphylococcus aureus (MRSA). In this direction, antimicrobial peptides (AMP) appears a promising solution. The aim of the present study was to inves...tigate the potential activity of Temporin A, Citropin 1.1, CA(1-7)M(2-9)NH2 and Pal-KGK-NH2 in synergic activity against MRSA biofilms developed on polystyrene surface (PSS) and central venous catheter (CVC).
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In vitro activity of Protegrin-1, alone and in combination with clinically useful antibiotics, against Acinetobacter baumannii strains isolated from surgical wounds
Anno: 2019
Rivista: MEDICAL MICROBIOLOGY AND IMMUNOLOGY
Autori/autrici: Morroni, Gianluca; Simonetti, Oriana; Brenciani, Andrea; Brescini, Lucia; Kamysz, Wojciech; Kamysz, Elzbieta; Neubauer, Damian; Caffarini, Miriam; Orciani, Monia; Giovanetti, Eleonora; Offidani, Annamaria; Giacometti, Andrea; Cirioni, Oscar
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/267244
Citazioni:
In vitro activity of Protegrin-1, alone and in combination with clinically useful antibiotics, against Acinetobacter baumannii strains isolated from surgical wounds / Morroni, G., Simonetti, O., Brenciani, A., Brescini, L., Kamysz, W., Kamysz, E., Neubauer, D., Caffarini, M., Orciani, M., Giovanetti, E., Offidani, A., Giacometti, A., Cirioni, O.. - In: MEDICAL MICROBIOLOGY AND IMMUNOLOGY. - ISSN 0300-8584. - ELETTRONICO. - 208:6(2019), pp. 877-883. [10.1007/s00430-019-00624-7]
Abstract:
In the past few years the increasing incidence of hospital infections with Acinetobacter baumannii, especially in immunocompromised patients, and its proneness to develop multidrug resistance have been raising considerable concern. This study examines the antimicrobial and antibiofilm activity of pr...otegrin 1 (PG-1), an antimicrobial peptide from porcine leukocytes, against A. baumannii strains isolated from surgical wounds. PG-1 was tested both alone and combined with the antibiotics commonly used in clinical settings. Its antimicrobial activity was evaluated by determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), checkerboard assays, and time-kill experiments. Its effects on biofilm inhibition/eradication were tested with crystal violet staining. The strains were grown in subinhibitory or increasing PG-1 concentrations to test the development of resistance. Mammalian cell toxicity was tested by XTT assays. PG-1 MICs and MBCs ranged from 2 to 8 µg/ml. PG-1 was most active and demonstrated a synergistic interaction with colistin, a last resort antibiotic. Interestingly, antagonism was never observed. In time-kill experiments, incubation with 2 × MIC for 30 min suppressed all viable cells. PG-1 did not select resistant strains and showed a limited effect on cell viability, but it did exert a strong activity against multidrug-resistant A. baumannii. In contrast, in our experimental conditions it had no effect on biofilm inhibition/eradication. PG-1 thus seems to be a promising antimicrobial agent against multidrug-resistant Gram-negative infections.
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Efficacy of pexiganan combination with tigecycline in a mouse model of Pseudomonas aeruginosa sepsis
Anno: 2018
Rivista: CURRENT TOPICS IN MEDICINAL CHEMISTRY
Autori/autrici: Cirioni, Oscar; Simonetti, Oriana; Morroni, Gianluca; Brescini, Lucia; Kamysz, Wojciech; Kamysz, Elzbieta; Orlando, Fiorenza; Pierpaoli, Elisa; Caffarini, Miriam; Orciani, Monia; Agostinelli, Claudio; Offidani, Annamaria; Provinciali, Mauro; Giacometti, Andrea
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/262566
Citazioni:
Efficacy of pexiganan combination with tigecycline in a mouse model of Pseudomonas aeruginosa sepsis / Cirioni, O., Simonetti, O., Morroni, G., Brescini, L., Kamysz, W., Kamysz, E., Orlando, F., Pierpaoli, E., Caffarini, M., Orciani, M., Agostinelli, C., Offidani, A., Provinciali, M., Giacometti, A.. - In: CURRENT TOPICS IN MEDICINAL CHEMISTRY. - ISSN 1568-0266. - 19:(2018). [10.2174/1568026619666181219123431]
Abstract:
Pseudomonas aeruginosa is a gram-negative pathogen, related with a severe mortality rate. It is also difficult to treat due to numerous resistance mechanisms to a wide range of antibiotics.

Antimicrobial Activity of Different Antimicrobial Peptides (AMPs) Against Clinical Methicillin-resistant Staphylococcus aureus (MRSA)
Anno: 2018
Rivista: CURRENT TOPICS IN MEDICINAL CHEMISTRY
Autori/autrici: Ciandrini, Eleonora; Morroni, Gianluca; Arzeni, Daniela; Kamysz, Wojciech; Neubauer, Damian; Kamysz, Elzbieta; Cirioni, Oscar; Brescini, Lucia; Baffone, Wally; Campana, Raffaella
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/265744
Citazioni:
Antimicrobial Activity of Different Antimicrobial Peptides (AMPs) Against Clinical Methicillin-resistant Staphylococcus aureus (MRSA) / Ciandrini, E., Morroni, G., Arzeni, D., Kamysz, W., Neubauer, D., Kamysz, E., Cirioni, O., Brescini, L., Baffone, W., Campana, R.. - In: CURRENT TOPICS IN MEDICINAL CHEMISTRY. - ISSN 1568-0266. - 18:24(2018), pp. 2116-2126. [10.2174/1568026618666181022140348]

Characterization of a Multiresistance Plasmid Carrying the optrA and cfr Resistance Genes From an Enterococcus faecium Clinical Isolate
Anno: 2018
Rivista: FRONTIERS IN MICROBIOLOGY
Autori/autrici: Morroni, Gianluca; Brenciani, Andrea; Antonelli, Alberto; Maria D’Andrea, Marco; Di Pilato, Vincenzo; Fioriti, Simona; Mingoia, Marina; Vignaroli, Carla; Cirioni, Oscar; Biavasco, Francesca; Varaldo, Pietro E.; Rossolini, Gian Maria; Giovanetti, Eleonora
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/260150
Citazioni:
Characterization of a Multiresistance Plasmid Carrying the optrA and cfr Resistance Genes From an Enterococcus faecium Clinical Isolate / Morroni, G., Brenciani, A., Antonelli, A., Maria D’Andrea, M., Di Pilato, V., Fioriti, S., Mingoia, M., Vignaroli, C., Cirioni, O., Biavasco, F., Varaldo, P.E., Rossolini, G.M., Giovanetti, E.. - In: FRONTIERS IN MICROBIOLOGY. - ISSN 1664-302X. - ELETTRONICO. - 9:2189(2018). [10.3389/fmicb.2018.02189]
Abstract:
Enterococcus faecium E35048, a bloodstream isolate from Italy, was the first strain where the oxazolidinone resistance gene optrA was detected outside China. The strain was also positive for the oxazolidinone resistance gene cfr. WGS analysis revealed that the two genes were linked (23.1 kb apart), ...being co-carried by a 41,816-bp plasmid that was named pE35048-oc. This plasmid also carried the macrolide resistance gene erm(B) and a backbone related to that of the well-known Enterococcus faecalis plasmid pRE25 (identity 96%, coverage 65%). The optrA gene context was original, optrA being part of a composite transposon, named Tn6628, which was integrated into the gene encoding for the ζ toxin protein (orf19 of pRE25). The cfr gene was flanked by two ISEnfa5 insertion sequences and the element was inserted into an lnu(E) gene. Both optrA and cfr contexts were excisable. pE35048-oc could not be transferred to enterococcal recipients by conjugation or transformation. A plasmid-cured derivative of E. faecium E35048 was obtained following growth at 42°C, and the complete loss of pE35048-oc was confirmed by WGS. pE35048-oc exhibited some similarity but also notable differences from pEF12-0805, a recently described enterococcal plasmid from human E. faecium also co-carrying optrA and cfr; conversely it was completely unrelated to other optrA- and cfr-carrying plasmids from Staphylococcus sciuri. The optrA-cfr linkage is a matter of concern since it could herald the possibility of a co-spread of the two genes, both involved in resistance to last resort agents such as the oxazolidinones.
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Spread of colistin resistance gene mcr-1 in Italy: characterization of the mcr-1.2 allelic variant in a colistin-resistant blood isolate of Escherichia coli
Anno: 2018
Rivista: DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
Autori/autrici: Simoni, Serena; Morroni, Gianluca; Brenciani, Andrea; Vincenzi, Chiara; Cirioni, Oscar; Castelletti, Sefora; Varaldo, Pietro E.; Giovanetti, Eleonora; Mingoia, Marina
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/252571
Citazioni:
Spread of colistin resistance gene mcr-1 in Italy: characterization of the mcr-1.2 allelic variant in a colistin-resistant blood isolate of Escherichia coli / Simoni, S., Morroni, G., Brenciani, A., Vincenzi, C., Cirioni, O., Castelletti, S., Varaldo, P.E., Giovanetti, E., Mingoia, M.. - In: DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE. - ISSN 0732-8893. - 91:1(2018), pp. 66-68. [10.1016/j.diagmicrobio.2017.12.015]
Abstract:
mcr-1.2, an allelic variant of the transferable colistin resistance gene mcr-1, was characterized in a colistin-resistant blood isolate of Escherichia coli. It was harbored by an IncX4-type plasmid (33,293 bp). Despite its low prevalence, the potentially worrying spread of the mcr-1 gene, particular...ly its mcr-1.2 variant, in Italy requires increasing surveillance.
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High rate of ceftobiprole resistance among clinical methicillin-resistant Staphylococcus aureus isolates from a hospital in central Italy
Anno: 2018
Rivista: ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
Autori/autrici: Morroni, Gianluca; Brenciani, Andrea; Brescini, Lucia; Fioriti, Simona; Simoni, Serena; Pocognoli, Antonella; Mingoia, Marina; Giovanetti, Eleonora; Barchiesi, Francesco; Giacometti, Andrea; Cirioni, Oscar
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/264133
Citazioni:
High rate of ceftobiprole resistance among clinical methicillin-resistant Staphylococcus aureus isolates from a hospital in central Italy / Morroni, G., Brenciani, A., Brescini, L., Fioriti, S., Simoni, S., Pocognoli, A., Mingoia, M., Giovanetti, E., Barchiesi, F., Giacometti, A., Cirioni, O.. - In: ANTIMICROBIAL AGENTS AND CHEMOTHERAPY. - ISSN 0066-4804. - 62:12(2018). [10.1128/AAC.01663-18]
Abstract:
Ceftobiprole is a fifth-generation cephalosporin with activity against methicillin-resistant Staphylococcus aureus (MRSA). One-year surveillance at the Regional Hospital of Ancona (Italy) disclosed a 12% ceftobiprole resistance rate (12/102 isolates; MIC, ≥4 mg/liter). Epidemiological char...acterization demonstrated that the resistant isolates all belonged to different clones. Penicillin-binding protein (PBP) analysis showed substitutions in all PBPs and a novel insertion in PBP2a. The mecB and mecC genes were not detected. Ceftobiprole susceptibility screening is essential to avoid therapeutic failure and the spread of ceftobiprole-resistant strains.
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In vitro and in vivo activity of fosfomycin alone and in combination with rifampin and tigecycline against Grampositive cocci isolated from surgical wound infections
Anno: 2018
Rivista: JOURNAL OF MEDICAL MICROBIOLOGY
Autori/autrici: Simonetti, Oriana; Morroni, Gianluca; Ghiselli, Roberto; Orlando, Fiorenza; Brenciani, Andrea; Xhuvelaj, Ledia; Provinciali, Mauro; Offidani, Annamaria; Guerrieri, Mario; Giacometti, Andrea; Cirioni, Oscar
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/252528
Citazioni:
In vitro and in vivo activity of fosfomycin alone and in combination with rifampin and tigecycline against Grampositive cocci isolated from surgical wound infections / Simonetti, O., Morroni, G., Ghiselli, R., Orlando, F., Brenciani, A., Xhuvelaj, L., Provinciali, M., Offidani, A., Guerrieri, M., Giacometti, A., Cirioni, O.. - In: JOURNAL OF MEDICAL MICROBIOLOGY. - ISSN 0022-2615. - ELETTRONICO. - 67:1(2018), pp. 139-143. [10.1099/jmm.0.000649]
Abstract:
Complicated skin and soft tissue infections constitute a heterogeneous group of severe disorders, with surgical site infections being the most common hospital-acquired ones. The aim of our study was to investigate the synergistic and bactericidal activities of antimicrobial combinations of fosfomyci...n with rifampicin and tigecycline against Enterococcus faecalis, Enterococcus faecium and methicillin-resistant Staphylococcus aureus (MRSA) clinical isolates, and also to evaluate their in vivo effects in a mouse wound infection model. In in vitro studies, the combinations of fosfomycin with rifampicin and tigecycline were both synergistic. These synergies were confirmed in in vivo studies: the drug combinations showed the highest antimicrobial effects compared to monotherapy. In conclusion, the efficacy of fosfomycin combinations, also confirmed in our in vivo model, may suggest new directions in the treatment of infected skin and a possible alternative way to control bacterial skin infection.
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Atazanavir/ritonavir with lamivudine as maintenance therapy in virologically suppressed HIV-infected patients: 96 week outcomes of a randomized trial
Anno: 2018
Rivista: JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
Autori/autrici: Fabbiani, Massimiliano; Gagliardini, Roberta; Ciccarelli, Nicoletta; Roldan, Eugenia Quiros; Latini, Alessandra; D'Ettorre, Gabriella; Antinori, Andrea; Castagna, Antonella; Orofino, Giancarlo; Francisci, Daniela; Chinello, Pierangelo; Madeddu, Giordano; Grima, Pierfrancesco; Rusconi, Stefano; Del Pin, Barbara; Lombardi, FRANCESCA ELENA; D'Avino, Alessandro; Focà, Emanuele; Colafigli, Manuela; Cauda, Roberto; Di Giambenedetto, Simona; De Luca, Andrea; Mondi, A.; Borghetti, A.; Baldonero, E.; Belmonti, S.; Lamonica, S.; Sidella, L.; Tamburrini, E.; Visconti, E.; Giacometti, A.; Barchiesi, F.; Castelli, P.; Cirioni, O.; Mazzocato, S.; DI PIETRO, Marinella; Blanc, P.; Degli Esposti, A.; Mariabelli, E.; Marini, Stefano; Poggi, A.; Quiros Roldan, E.; Amadasi, S.; Apostoli, A.; Biasi, L.; Bonito, A.; Brianese, N.; Compostella, S.; Ferraresi, A.; Motta, D.; Mughini, M. T.; Celesia, B. M.; Gussio, M.; Sofia, S.; Tana, M.; Tundo, P.; Viscoli, C.; De Hoffer, L.; Di Biagio, A.; Grignolo, S.; Parisini, A.; Schenone, E.; Taramasso, L.; Manconi, P. E.; Boccone, A.; Ortu, F.; Piano, P.; Serusi, L.; Puoti, M.; Moioli, M. C.; Rossotti, R.; Travi, G.; Ventura, Fiammetta; Galli, M.; Di Nardo Stuppino, S.; Di Cristo, V.; Giacomelli, Andrea; Vimercati, V.; Viale, P.; Gori, A.; Rizzardini, G.; Capetti, A.; Carenzi, L.; Mazza, F.; Meraviglia, P.; DE ROSA, Stefano; Zucchi, Paolo; Mineo, Martina; Giuliani, M.; Pacifici, A.; Pimpinelli, F.; Solivetti, F.; Stivali, F.; Angelici, Francesca; Bellagamba, Roberta; Delle Rose, D.; Fezza, R.; Libertone, R.; Mosti, S.; Narciso, P.; Nicastri, E.; Ottou, S.; Tomassi, C.; Vlassi, C.; Zaccarelli, M.; Zoppè, F.; Vullo, V.; Altavilla, F.; Ceccarelli, Gabriele; Fantauzzi, A.; Gebremeskel, S.; Lo Menzo, S.; Mezzaroma, I.; Tierno, F.; Petrosillo, N.; Boumis, E.; Cicalini, S.; Grilli, E.; Musso, M.; Stella, C.; Mura, M. S.; Bagella, P.; Mannazzu, M.; Soddu, V.; Caramello, P.; Carcieri, C.; Carosella, S.; Farenga, M.; Scotton, P. G.; Rossi, M. C.; Concia, E.; Corsini, F.; Gricolo, C.; Lanzafame, M.; Lattuada, E.; Leonardi, S.; Rigo, F.; Lazzarin, A.; Bigoloni, A.; Carini, E.; Nozza, S.; Spagnuolo, V.; Belfiori, B.; Malincarne, L.; Schiaroli, E.; Sfara, C.; Tosti, A.; Sacchini, D.; Ruggieri, A.; Valdatta, C.
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/264136
Citazioni:
Atazanavir/ritonavir with lamivudine as maintenance therapy in virologically suppressed HIV-infected patients: 96 week outcomes of a randomized trial / Fabbiani, M., Gagliardini, R., Ciccarelli, N., Roldan, E.Q., Latini, A., D'Ettorre, G., Antinori, A., Castagna, A., Orofino, G., Francisci, D., Chinello, P., Madeddu, G., Grima, P., Rusconi, S., Del Pin, B., Lombardi, F.E., D'Avino, A., Focà, E., Colafigli, M., Cauda, R., et al.. - In: JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY. - ISSN 0305-7453. - STAMPA. - 73:7(2018), pp. 1955-1964. [10.1093/jac/dky123]
Abstract:
Objectives: To investigate the long-term safety and efficacy of a treatment switch to dual ART with atazanavir/ritonavir + lamivudine versus continuing a standard regimen with atazanavir/ritonavir + 2NRTI in virologically suppressed patients. Methods: ATLAS-M is a ...96 week open-label, randomized, non-inferiority (margin -12%) trial enrolling HIV-infected adults on atazanavir/ritonavir + 2NRTI, with stable HIV-RNA <50 copies/mL and CD4 counts >200 cells/mm3. At baseline, patients were randomized 1:1 to switch to atazanavir/ritonavir + lamivudine or to continue the previous regimen. Here, we report the 96 week efficacy and safety data. The study was registered with ClinicalTrials.gov, number NCT01599364. Results: Overall, 266 subjects were enrolled (133 in each arm). At 96 weeks, in the ITT population, patients free of treatment failure totalled 103 (77.4%) with atazanavir/ritonavir + lamivudine and 87 (65.4%) with triple therapy (difference +12.0%, 95% CI +1.2/+22.8, P = 0.030), demonstrating the superiority of dual therapy. Two (1.5%) and 9 (6.8%) virological failures occurred in the dual-therapy arm and the triple-therapy arm, respectively, without development of resistance to any study drug. Clinical adverse events occurred at similar rates in both arms. A higher frequency of grade 3-4 hyperbilirubinemia (66.9% versus 50.4%, P = 0.006) and hypertriglyceridaemia (6.8% versus 1.5%, P = 0.031) occurred with dual therapy, although this never led to treatment discontinuation. A significant improvement in renal function and lumbar spine bone mineral density occurred in the dual-therapy arm. The evolution of CD4, HIV-DNA levels and neurocognitive performance was similar in both arms. Conclusions: In this randomized study, a treatment switch to atazanavir/ritonavir + lamivudine was superior over the continuation of atazanavir/ritonavir + 2NRTI in virologically suppressed patients, with a sustained benefit in terms of improved renal function and bone mineral density.
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Supplementation with tocotrienols from Bixa orellana improves the in vivo efficacy of daptomycin against methicillin-resistant Staphylococcus aureus in a mouse model of infected wound
Anno: 2017
Rivista: PHYTOMEDICINE
Autori/autrici: Pierpaoli, Elisa; Orlando, Fiorenza; Cirioni, Oscar; Simonetti, Oriana; Giacometti, Andrea; Provinciali, Mauro
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/257985
Citazioni:
Supplementation with tocotrienols from Bixa orellana improves the in vivo efficacy of daptomycin against methicillin-resistant Staphylococcus aureus in a mouse model of infected wound / Pierpaoli, E., Orlando, F., Cirioni, O., Simonetti, O., Giacometti, A., Provinciali, M.. - In: PHYTOMEDICINE. - ISSN 0944-7113. - STAMPA. - 36:(2017), pp. 50-53. [10.1016/j.phymed.2017.09.011]

Effect of omiganan on colonic anastomosis healing in a rat model of peritonitis
Anno: 2017
Rivista: AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH
Autori/autrici: Lorenzi, Teresa; Trombettoni, Maria Michela Cappelletti; Ghiselli, Roberto; Paolinelli, Francesca; Gesuita, Rosaria; Cirioni, Oscar; Provinciali, Mauro; Kamysz, Wojciech; Kamysz, Elzbieta; Piangatelli, Cristiano; Castellucci, Mario; Guerrieri, Mario; Morroni, Manrico
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/250253
Citazioni:
Effect of omiganan on colonic anastomosis healing in a rat model of peritonitis / Lorenzi, T., Trombettoni, M.M.C., Ghiselli, R., Paolinelli, F., Gesuita, R., Cirioni, O., Provinciali, M., Kamysz, W., Kamysz, E., Piangatelli, C., Castellucci, M., Guerrieri, M., Morroni, M.. - In: AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH. - ISSN 1943-8141. - ELETTRONICO. - 9:7(2017), pp. 3374-3386.
Abstract:
Background: This study investigates the effects of the antimicrobial cationic peptide omiganan - alone and combined with the antibiotic imipenem - on colonic anastomosis healing in presence of intraperitoneal sepsis induced in a rodent model of cecal ligation and puncture (CLP). Methods: Forty male... Wistar rats were divided into 5 groups of 8 animals. Group 1 (control group) underwent laparotomy and cecal mobilization and the next day received left colon anastomosis. In group 2 (CLP without treatment), group 3 (CLP + imipenem), group 4 (CLP + omiganan), and group 5 (CLP + omiganan + imipenem), the left colon anastomosis was performed the day after CLP. Imipenem and omiganan were administered by intraperitoneal injection immediately before anastomosis construction and subsequently at 24 h intervals until the 7th postoperative day, when rats were sacrificed. Anastomotic bursting pressure was measured in situ. Tissue samples were collected for determination of hydroxyproline content and histological characteristics. Results: Only rats receiving omiganan + imipenem displayed re-epithelialization, reduced neovascularization of granulation tissue, and a bursting pressure that was similar to that of controls. Omiganan - alone and combined with imipenem - was associated with a better control of inflammatory parameters than imipenem alone. In addition omiganan, like imipenem, counteracted the collagen depletion typical of sepsis. Conclusions: This experimental study demonstrates the efficacy of the new antimicrobial agent omiganan, alone and in combination with imipenem, in delaying the effects of intraperitoneal sepsis on colonic anastomosis healing and provides evidence of the value of omiganan as a therapeutic agent.
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Role of daptomycin on burn wound healing in an animal methicillin-resistant staphylococcus aureus infection model
Anno: 2017
Rivista: ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
Autori/autrici: Simonetti, O; Lucarini, G; Orlando, F; Pierpaoli, E; Ghiselli, R; Provinciali, M; Castelli, P; Guerrieri, M; Di Primio, R; Offidani, A; Giacometti, A; Cirioni, O.
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/255196
Citazioni:
Role of daptomycin on burn wound healing in an animal methicillin-resistant staphylococcus aureus infection model / Simonetti, O., Lucarini, G., Orlando, F., Pierpaoli, E., Ghiselli, R., Provinciali, M., Castelli, P., Guerrieri, M., Di Primio, R., Offidani, A., Giacometti, A., Cirioni, O.. - In: ANTIMICROBIAL AGENTS AND CHEMOTHERAPY. - ISSN 0066-4804. - 61:9(2017).

HIV-1 DNA dynamics and variations in HIV-1 DNA protease and reverse transcriptase sequences in multidrug-resistant patients during successful raltegravir-based therapy
Anno: 2016
Rivista: JOURNAL OF MEDICAL VIROLOGY
Autori/autrici: Michelini, Z; Galluzzo, Cm; Pirillo, Mf; Francisci, D; Degli Antoni, A; Vivarelli, A; Ladisa, N; Cirioni, Oscar; Weimer, Le; Fragola, V; Cara, A; Floridia, M; Baroncelli, S.
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/248746
Citazioni:
HIV-1 DNA dynamics and variations in HIV-1 DNA protease and reverse transcriptase sequences in multidrug-resistant patients during successful raltegravir-based therapy / Michelini, Z., Galluzzo, C.m., Pirillo, M.f., Francisci, D., Degli Antoni, A., Vivarelli, A., Ladisa, N., Cirioni, O., Weimer, L.e., Fragola, V., Cara, A., Floridia, M., Baroncelli, S.. - In: JOURNAL OF MEDICAL VIROLOGY. - ISSN 0146-6615. - STAMPA. - 88:(2016), pp. -2115-2124.

Effects of the Infusion of 4% or 20% Human Serum Albumin on the Skeletal Muscle Microcirculation in Endotoxemic Rats
Anno: 2016
Rivista: PLOS ONE
Autori/autrici: Damiani, Elisa; Ince, Can; Orlando, Fiorenza; Pierpaoli, Elisa; Cirioni, Oscar; Giacometti, Andrea; Mocchegiani, Federico; Pelaia, Paolo; Provinciali, Mauro; Donati, Abele
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/232981
Citazioni:
Effects of the Infusion of 4% or 20% Human Serum Albumin on the Skeletal Muscle Microcirculation in Endotoxemic Rats / Damiani, E., Ince, C., Orlando, F., Pierpaoli, E., Cirioni, O., Giacometti, A., Mocchegiani, F., Pelaia, P., Provinciali, M., Donati, A.. - In: PLOS ONE. - ISSN 1932-6203. - ELETTRONICO. - 11:3(2016), p. e0151005. [10.1371/journal.pone.0151005]
Abstract:
Sepsis-induced microcirculatory alterations contribute to tissue hypoxia and organ dysfunction. In addition to its plasma volume expanding activity, human serum albumin (HSA) has anti-oxidant and anti-inflammatory properties and may have a protective role in the microcirculation during sepsis. The c...oncentration of HSA infused may influence these effects. We compared the microcirculatory effects of the infusion of 4% and 20% HSA in an experimental model of sepsis.
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Efficacy of the quorum sensing inhibitor FS10 alone and in combination with tigecycline in an animal model of staphylococcal infected wound
Anno: 2016
Rivista: PLOS ONE
Autori/autrici: Simonetti, Oriana; Cirioni, Oscar; Cacciatore, Ivana; Baldassarre, Leonardo; Orlando, Fiorenza; Pierpaoli, Elisa; Lucarini, Guendalina; Orsetti, Elena; Provinciali, Mauro; Fornasari, Erika; Di Stefano, Antonio; Giacometti, Andrea; Offidani, Annamaria
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/237807
Citazioni:
Efficacy of the quorum sensing inhibitor FS10 alone and in combination with tigecycline in an animal model of staphylococcal infected wound / Simonetti, O., Cirioni, O., Cacciatore, I., Baldassarre, L., Orlando, F., Pierpaoli, E., Lucarini, G., Orsetti, E., Provinciali, M., Fornasari, E., Di Stefano, A., Giacometti, A., Offidani, A.. - In: PLOS ONE. - ISSN 1932-6203. - ELETTRONICO. - 11:6(2016), p. e0151956. [10.1371/journal.pone.0151956]
Abstract:
In staphylococci, quorum sensing regulates both biofilm formation and toxin production, moreover it has been demonstrated to be inhibited by RNAIII inhibiting peptide (RIP). Aim our study was to evaluate the in vitro activity and its in vivo efficacy of the combined administration of FS10, a novel R...IP derivative, and tigecycline in an animal model of methicillin-resistant (MR) and methicillin-sensitive (MS) Staphylococcus aureus wound infection. Using a 1.x2 cm template, one full thickness wound was established through the panniculus carnosus on the back subcutaneous tissue of each animal. Infection was determined by inoculation of 5x107 CFU/ml of bacteria, that produced an abscess within 24 h, after this, treatment was initiated. The study included, for each strain, a control group without infection, a control infected group that did not receive any treatment and a control infected group with drug-free foam dressing, and three infected groups treated, respectively, with: FS10-soaked foam dressing (containing 20 μg FS10), daily intraperitoneal tigecycline (7 mg/Kg), FS10-soaked foam dressing (containing 20 μg FS10) and daily intraperitoneal injections of tigecycline (7 mg/Kg). The main outcome measures were quantitative culture and histological examination of tissue repair. The highest inhibition of infection was achieved in the group that received FS10-soaked and parenteral tigecycline reducing the bacterial load from 107 CFU/ml to about 103 CFU/g for MSSA and to about 104 CFU/g for MRSA. The group treated with FS10-soaked foam dressing associated with parenteral tigecycline showed, histologically, better overall healing with epithelialization and collagen scores significantly higher than those of the other groups in both strains. In conclusion, the combined use of topical FS10 with i.p. tigecycline induced positive interaction in vivo, resulting in an enhanced therapeutic benefit versus staphylococcal infections in murine wound models.
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Colistin enhances therapeutic efficacy of daptomycin or teicoplanin in a murine model of multiresistant Acinetobacter baumannii sepsis.
Anno: 2016
Rivista: DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
Autori/autrici: Cirioni, Oscar; Simonetti, Oriana; Pierpaoli, Elisa; Barucca, A; Ghiselli, Roberto; Orlando, Fiorenza; Pelloni, Maria; Trombettoni, Mm; Guerrieri, Mario; Offidani, Annamaria; Giacometti, Andrea; Provinciali, M.
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/241231
Citazioni:
Colistin enhances therapeutic efficacy of daptomycin or teicoplanin in a murine model of multiresistant Acinetobacter baumannii sepsis / Cirioni, O., Simonetti, O., Pierpaoli, E., Barucca, A., Ghiselli, R., Orlando, F., Pelloni, M., Trombettoni, M.m., Guerrieri, M., Offidani, A., Giacometti, A., Provinciali, M.. - In: DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE. - ISSN 0732-8893. - STAMPA. - 86:4(2016), pp. 392-398. [10.1016/j.diagmicrobio.2016.09.010]
Abstract:
We investigated the efficacy of colistin combined with teicoplanin or daptomycin in an experimental mouse model of multiresistant Acinetobacter baumannii infection. Animal received intraperitoneally 1ml saline containing 2×1010CFU of A. baumannii. Colistin, daptomycin, teicoplanin, and colisti...n plus daptomycin or teicoplanin were given by intraperitoneal administration 2h after bacterial challenge. A control group received sodium chloride solution. In the in vitro study A. baumannii showed to be susceptible only to colistin with MIC of 2mg/l. In the in vivo study, colistin alone showed a good antimicrobial efficacy. When combined with teicoplanin or daptomycin, colistin produced the lowest bacterial and the best survival rates. In immunological studies, when colistin was associated to daptomycin or teicoplanin, both the number and the cytotoxic activity of NK cells increased. In conclusion, colistin combined with teicoplanin or daptomycin may improve the therapy of multiresistant A. baumannii infection.
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Enhanced efficacy of combinations of pexiganan with colistin versus Acinetobacter baumannii in experimental sepsis
Anno: 2016
Rivista: SHOCK
Autori/autrici: Cirioni, Oscar; Simonetti, Oriana; Pierpaoli, Elisa; Barucca, Alessandra; Ghiselli, Roberto; Orlando, Fiorenza; Pelloni, Maria; Minardi, Daniele; Trombettoni, Maria Michela Cappelletti; Guerrieri, Mario; Offidani, Annamaria; Giacometti, Andrea; Provinciali, Mauro
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/237808
Citazioni:
Enhanced efficacy of combinations of pexiganan with colistin versus Acinetobacter baumannii in experimental sepsis / Cirioni, O., Simonetti, O., Pierpaoli, E., Barucca, A., Ghiselli, R., Orlando, F., Pelloni, M., Minardi, D., Trombettoni, M.M.C., Guerrieri, M., Offidani, A., Giacometti, A., Provinciali, M.. - In: SHOCK. - ISSN 1073-2322. - STAMPA. - 46:2(2016), pp. 1-8. [10.1097/SHK.0000000000000584]
Abstract:
We investigated the efficacy of colistin combined with pexiganan in experimental mouse models of Acinetobacter baumannii infection.Adult male BALB/c mice received intraperitoneally 1 mL saline containing 2 × 10 CFU of susceptible and multiresistant A. baumannii. Two hours after bacterial c...hallenge, animals received 1 mg/kg of colistin, 1 mg/kg of pexiganan, or 1 mg/kg of colistin plus 1 mg/kg of pexiganan.Blood culture positivity, the quantities of bacteria in the intra-abdominal fluid, the rate of lethality and immunological studies, such as immunophenotyping and NK cytotoxicity, were evaluated.In the in vitro study, A. baumannii showed susceptibility to colistin and pexiganan and a strong synergy was observed by testing colistin combined with pexiganan with fractionary inhibitory concentration index of 0.312 for both strains.In the in vivo study colistin or pexiganan alone showed a good antimicrobial efficacy. When colistin was combined with pexiganan, the positive interaction produced low bacterial counts that were statistically significant versus singly treated groups. For both strains the highest rate of survival was observed in combined-treated groups (90%).Pexiganan increased NK cytotoxic activity over the levels of infected and colistin-treated animals.In conclusion, pexiganan combined with colistin was found to be efficacious against A. baumannii infection.
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Rate and determinants of residual viremia in multidrug-experienced patients successfully treated with raltegravir-based regimens
Anno: 2015
Rivista: AIDS RESEARCH AND HUMAN RETROVIRUSES
Autori/autrici: Baroncelli, Silvia; Pirillo, Maria Franca; Galluzzo, Clementina Maria; Antoni, Anna Degli; Ladisa, Nicoletta; Francisci, Daniela; D'Ettorre, Gabriella; Segala, Daniela; Vivarelli, Angela; Sozio, Federica; Cirioni, Oscar; Weimer, Liliana Elena; Fragola, Vincenzo; Parruti, Giustino; Floridia, Marco
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/234437
Citazioni:
Rate and determinants of residual viremia in multidrug-experienced patients successfully treated with raltegravir-based regimens / Baroncelli, S., Pirillo, M.F., Galluzzo, C.M., Antoni, A.D., Ladisa, N., Francisci, D., D'Ettorre, G., Segala, D., Vivarelli, A., Sozio, F., Cirioni, O., Weimer, L.E., Fragola, V., Parruti, G., Floridia, M.. - In: AIDS RESEARCH AND HUMAN RETROVIRUSES. - ISSN 0889-2229. - ELETTRONICO. - 31:1(2015), pp. 71-77. [10.1089/AID.2014.0060]
Abstract:
Residual HIV viremia, defined by low levels of plasma HIV RNA with enhanced-sensitivity assays, may persist even in the presence of successful antiretroviral therapy, but little is known about its determinants. Our objective was to evaluate the rate and determinants of residual viremia in patients w...ho show stable undetectable plasma HIV-1 RNA with conventional assays. Forty-four multidrug-experienced patients with undetectable levels of HIV RNA for at least 2 years under raltegravir-based regimens were evaluated. An ultrasensitive (2.5 copies/ml) real-time PCR method was used to quantify plasma HIV RNA. After 12 months of salvage treatment, 48.3% of the patients had residual viremia between 2.5 and 37 copies/ml. The proportion of patients with plasma HIV RNA below 2.5 copies/ml decreased from 51.7% at 12 months to 30.8% at 24 months. The presence of residual viremia was not associated with levels of viremia before starting raltegravir. Considering CD4 counts, hepatitis B or C virus (HBV or HCV) coinfection, or other demographic characteristics, for the time interval between HIV diagnosis and initiation of antiretroviral therapy, patients with a longer interval (>1 year) were significant less likely to have RNA levels below 2.5 copies/ml at 12 months compared to patients who started therapy within 1 year of HIV diagnosis (28.6% vs. 73.3%, p=0.027). Half of the patients showing undetectable HIV viremia with conventional assays had low-level viremia with ultrasensitive assays, with no predictive role of viroimmunological status at the start of the regimen. The potential influence of the interval between HIV diagnosis and initiation of treatment should be confirmed in subjects with a known date of seroconversion.
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Antiretroviral therapy management and rationalisation of available resources
Anno: 2015
Rivista: LE INFEZIONI IN MEDICINA
Autori/autrici: Cirioni, Oscar; Castelletti, Sefora; Ucciferri, Claudio; Falasca, Katia; Orsetti, Elena; Mazzocato, Susanna; Valeriani, Chiara; Di Campli, Francesco Maria; Barchiesi, Francesco; Vecchiet, Jacopo; Giacometti, Andrea
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/230453
Citazioni:
Antiretroviral therapy management and rationalisation of available resources / Cirioni, O., Castelletti, S., Ucciferri, C., Falasca, K., Orsetti, E., Mazzocato, S., Valeriani, C., Di Campli, F.M., Barchiesi, F., Vecchiet, J., Giacometti, A.. - In: LE INFEZIONI IN MEDICINA. - ISSN 1124-9390. - ELETTRONICO. - 23:4(2015), p. 330-5.
Abstract:
The treatment of HIV disease has led to a new division of management costs by shifting most of the necessary resources from inpatient treatment to outpatient management. Among the initiatives aimed at rationalising the resources available, we compared efficacy, tolerability and pharmacoeconomic impa...ct of different regimes of antiretroviral therapy (ART). The survey covered the first 50 patients, clinically stable and with good viro-immunological response, who switched in June 2012 from an ART based on the triple combination of tenofovir (TDF), emtricitabine (FTC) and a protease inhibitor boosted with ritonavir (PI/r) or a non-nucleoside reverse transcriptase inhibitor (NNRTI), to a treatment based on abacavir (ABC), lamivudine (3TC) and a PI/r or NNRTI. Of the 50 patients who operated the switch, 39 replaced a PI with nevirapine (NVP), for which the largest group of patients was treated with ABC + 3TC + NVP. On 31 May 2015, all patients completed the observation period of 96 weeks, with a mean observation period of 132 weeks and clinical-laboratory checks every four months. Laboratory analysis revealed an optimal maintenance of viral suppression and absolute and relative number of CD4 + lymphocytes and improving trend of creatinine, proteinuria, serum phosphate and bone alkaline phosphatase. There was a variable effect on lipids, with a drop in triglycerides associated with a modest increase in total cholesterol. Much of the HIV-positive population reporting to our hospitals (>50%) comprises individuals who have for years been in stable viraemic suppression, making a satisfactory immune recovery while in good overall clinical condition. This type of patient was the target of the present survey. At the end of 96 weeks of observation the new regimes were well tolerated and did not lead to viro-immunological or clinical deterioration. Pharmacoeconomic analysis showed better containment of the overall costs. No patient needed to be hospitalised during the observation period.
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In vitro activity and in vivo animal model efficacy of IB-367 alone and in combination with imipenem and colistin against Gram-negative bacteria.
Anno: 2014
Rivista: PEPTIDES
Autori/autrici: Simonetti, Oriana; Cirioni, Oscar; Ghiselli, Roberto; Orlando, F; Silvestri, Carmela; Mazzocato, Susanna; Kamysz, W; Kamysz, E; Provinciali, M; Giacometti, Andrea; Guerrieri, Mario; Offidani, Annamaria
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/225176
Citazioni:
In vitro activity and in vivo animal model efficacy of IB-367 alone and in combination with imipenem and colistin against Gram-negative bacteria / Simonetti, O., Cirioni, O., Ghiselli, R., Orlando, F., Silvestri, C., Mazzocato, S., Kamysz, W., Kamysz, E., Provinciali, M., Giacometti, A., Guerrieri, M., Offidani, A.. - In: PEPTIDES. - ISSN 0196-9781. - STAMPA. - 55:(2014), pp. 17-22. [10.1016/j.peptides.2014.01.029]
Abstract:
The aim of our study was to evaluate the in vitro activity of IB-367 and its bactericidal effect for Pseudomonas aeruginosa and Escherichia coli, associated to a synergic study to test the antibiotic combinations between the peptide and colistin or imipenem. Minimum inhibitory concentrations (MICs),... the minimum bactericidal concentrations (MBCs), the synergy test and killing study were carried out to evaluate the IB-367 activity. In the in vivo model, a wound was incised through the panniculus carnosus of BALB/c mice, and then inoculated with 5 × 107 colony-forming units of P. aeruginosa and E. coli. For each strain, the study included an infected or not infected group that did not receive any treatment, and five contaminated groups treated with local IB- 367, intraperitoneal imipenem, intraperitoneal colistin, topical IB-367 local plus intraperitoneal imipenem or intraperitoneal colistin. All isolates were inhibited by IB-367 at concentrations of 4-64 mg/l. Killing by IB-367 was shown to be very rapid: its activity on all Gram-negative bacteria was completed within a 40 min exposure period at a concentration of 2 × MIC/l. Synergy was demonstrated when IB-367 was combined with colistin or imipenem. In in vivo studies, the groups treated with topical IB-367 and intraperitoneal colistin showed the best results in terms of bacterial load inhibition either for Pseudomonas or for E. coli. The good in vitro activity and in vivo efficacy, as well as, the synergic interactions with antibiotics suggest that IB-367 is a promising candidate for potential application in the treatment of wound Gram-negative infections.
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Relationship between health-related quality of life measures and high HIV viral load in HIV-infected triple-class-experienced patients
Anno: 2014
Rivista: HIV CLINICAL TRIALS
Autori/autrici: Bucciardini, R; Pugliese, K; Weimer, L; Digregorio, M; Fragola, V; Mancini, M; Maroccia, Z; Ladisa, N; Francisci, D; Bellagamba, R; Degli Antoni, A; Guaraldi, G; Cirioni, Oscar; Ortu, F; Parruti, G; Mannazzu, M; Libertone, R; Donnini, S; Floridia, M.
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/248748
Citazioni:
Relationship between health-related quality of life measures and high HIV viral load in HIV-infected triple-class-experienced patients / Bucciardini, R., Pugliese, K., Weimer, L., Digregorio, M., Fragola, V., Mancini, M., Maroccia, Z., Ladisa, N., Francisci, D., Bellagamba, R., Degli Antoni, A., Guaraldi, G., Cirioni, O., Ortu, F., Parruti, G., Mannazzu, M., Libertone, R., Donnini, S., Floridia, M.. - In: HIV CLINICAL TRIALS. - ISSN 1528-4336. - STAMPA. - 15:(2014), pp. 176-183.

In vitro activity of the protegrin IB-367 alone and in combination compared with conventional antifungal agents against dermatophytes
Anno: 2014
Rivista: MYCOSES
Autori/autrici: Simonetti, Oriana; Silvestri, Carmela; Arzeni, Daniela; Cirioni, Oscar; Kamysz, Wojciech; Conte, Irene; Staffolani, Silvia; Orsetti, Elena; Morciano, Angela; Castelli, Pamela; Scalise, Alessandro; Kamysz, Elzbieta; Offidani, Annamaria; Giacometti, Andrea; Barchiesi, Francesco
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/205739
Citazioni:
In vitro activity of the protegrin IB-367 alone and in combination compared with conventional antifungal agents against dermatophytes / Simonetti, O., Silvestri, C., Arzeni, D., Cirioni, O., Kamysz, W., Conte, I., Staffolani, S., Orsetti, E., Morciano, A., Castelli, P., Scalise, A., Kamysz, E., Offidani, A., Giacometti, A., Barchiesi, F.. - In: MYCOSES. - ISSN 0933-7407. - STAMPA. - 57:4(2014), pp. 233-239. [10.1111/myc.12148]

Urinary catheters and biofilm formation
Anno: 2013
Rivista: RECENT PATENTS ON BIOMARKERS
Autori/autrici: Minardi, Daniele; Conti, Alessandro; Santoni, Matteo; Cantoro, Daniele; Cirioni, Oscar; Ghiselli, Roberto; Silvestri, Carmela; Guerrieri, Mario; Giacometti, Andrea; Muzzonigro, Giovanni
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/146307
Citazioni:
Urinary catheters and biofilm formation / Minardi, D., Conti, A., Santoni, M., Cantoro, D., Cirioni, O., Ghiselli, R., Silvestri, C., Guerrieri, M., Giacometti, A., Muzzonigro, G.. - In: RECENT PATENTS ON BIOMARKERS. - ISSN 2210-3090. - STAMPA. - 3:(2013), pp. 196-203. [10.2174/22103090113036660010]

IB-367 pre-treatment improves the in vivo efficacy of teicoplanin and daptomycin in an animal model of wounds infected with meticillin-resistant Staphylococcus aureus
Anno: 2013
Rivista: JOURNAL OF MEDICAL MICROBIOLOGY
Autori/autrici: Cirioni, O.; Silvestri, C.; Pierpaoli, E.; Barucca, A.; Kamysz, W.; Ghiselli, R.; Scalise, A.; Brescini, L.; Castelli, P.; Orlando, F.; Kamysz, E.; Guerrieri, M.; Giacometti, A.; Provinciali, M.
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/160909
Citazioni:
IB-367 pre-treatment improves the in vivo efficacy of teicoplanin and daptomycin in an animal model of wounds infected with meticillin-resistant Staphylococcus aureus / Cirioni, O., Silvestri, C., Pierpaoli, E., Barucca, A., Kamysz, W., Ghiselli, R., Scalise, A., Brescini, L., Castelli, P., Orlando, F., Kamysz, E., Guerrieri, M., Giacometti, A., Provinciali, M.. - In: JOURNAL OF MEDICAL MICROBIOLOGY. - ISSN 0022-2615. - STAMPA. - 62:10(2013), pp. 1552-1558.

Quorum Sensing Inhibitor FS3-coated Vascular Graft Enhances Daptomycin Efficacy in a Rat Model of Staphylococcal Biofilm Infection.
Anno: 2013
Rivista: PEPTIDES
Autori/autrici: Cirioni, Oscar; Mocchegiani, Federico; Cacciatore, I; Vecchiet, J; Silvestri, Carmela; Baldassarre, L; Ucciferri, C; Orsetti, E; Castelli, P; Provinciali, M; Vivarelli, Marco; Fornasari, E; Giacometti, Andrea
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/156114
Citazioni:
Quorum Sensing Inhibitor FS3-coated Vascular Graft Enhances Daptomycin Efficacy in a Rat Model of Staphylococcal Biofilm Infection / Cirioni, O., Mocchegiani, F., Cacciatore, I., Vecchiet, J., Silvestri, C., Baldassarre, L., Ucciferri, C., Orsetti, E., Castelli, P., Provinciali, M., Vivarelli, M., Fornasari, E., Giacometti, A.. - In: PEPTIDES. - ISSN 0196-9781. - STAMPA. - 40:(2013), pp. 77-81.

Sustained increase of serum creatine phosphokinase levels and progressive muscle abnormalities associated with raltegravir use during 32-week follow-up in an HIV-1 experienced patient on simplified HAART regimen, intolerant to protease inhibitors and abacavir: a case report.
Anno: 2013
Rivista: WEST INDIAN MEDICAL JOURNAL
Autori/autrici: Cirioni, Oscar; Weimer, Liliana E; Fragola, Vincenzo; Giacometti, Andrea; Gabrielli, Eleonora; Marchionni, Elisa; Massella, M; Barchiesi, Francesco
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/230417
Citazioni:
Sustained increase of serum creatine phosphokinase levels and progressive muscle abnormalities associated with raltegravir use during 32-week follow-up in an HIV-1 experienced patient on simplified HAART regimen, intolerant to protease inhibitors and abacavir: a case report / Cirioni, O., Weimer, L.E., Fragola, V., Giacometti, A., Gabrielli, E., Marchionni, E., Massella, M., Barchiesi, F.. - In: WEST INDIAN MEDICAL JOURNAL. - ISSN 0043-3144. - ELETTRONICO. - (2013). [10.7727/WIMJ.2011.193.]
Abstract:
Sustained increase of serum creatine phosphokinase (CPK) concentrations and muscle abnormalities have been reported in patients taking raltegravir (RAL). In this report, we describe a case of sustained and asymptomatic increase of serum CPK concentrations associated with raltegravir, zidovudine, and... lamivudine in an HIV-1 experienced patient with intolerance to protease inhibitor, abacavir and penicillin during 32 weeks of continuous drug monitoring.
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The efficacy of the quorum sensing inhibitor FS8 and tigecycline in preventing prosthesis biofilm in an animal model of staphylococcal infection
Anno: 2013
Rivista: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Autori/autrici: Simonetti, Oriana; Cirioni, Oscar; Mocchegiani, Federico; Cacciatore, I; Silvestri, C; Baldassarre, L; Orlando, F; Castelli, P; Provinciali, M; Vivarelli, Marco; Fornasari, E; Giacometti, Andrea; Offidani, Annamaria
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/156111
Citazioni:
The efficacy of the quorum sensing inhibitor FS8 and tigecycline in preventing prosthesis biofilm in an animal model of staphylococcal infection / Simonetti, O., Cirioni, O., Mocchegiani, F., Cacciatore, I., Silvestri, C., Baldassarre, L., Orlando, F., Castelli, P., Provinciali, M., Vivarelli, M., Fornasari, E., Giacometti, A., Offidani, A.. - In: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. - ISSN 1661-6596. - STAMPA. - 14:8(2013), pp. 16321-16332. [10.3390/ijms140816321]

Delayed wound healing in aged skin rat models after thermal injury is associated with an increased MMP-9, K6 and CD44 expression
Anno: 2013
Rivista: BURNS
Autori/autrici: Simonetti, Oriana; Lucarini, Guendalina; Cirioni, Oscar; Zizzi, Antonio; Orlando, F; Provinciali, M; DI PRIMIO, Roberto; Giacometti, Andrea; Offidani, Annamaria
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/156112
Citazioni:
Delayed wound healing in aged skin rat models after thermal injury is associated with an increased MMP-9, K6 and CD44 expression / Simonetti, O., Lucarini, G., Cirioni, O., Zizzi, A., Orlando, F., Provinciali, M., DI PRIMIO, R., Giacometti, A., Offidani, A.. - In: BURNS. - ISSN 0305-4179. - 39:4(2013), pp. 776-787. [10.1016/j.burns.2012.09.013]

In vitro and in vivo effects of sub-MICs of pexiganan and imipenem on Pseudomonas aeruginosa adhesion and biofilm development.
Anno: 2013
Rivista: LE INFEZIONI IN MEDICINA
Autori/autrici: Cirioni, Oscar; Silvestri, Carmela; Ghiselli, Roberto; Kamysz, W; Minardi, Daniele; Castelli, Pamela; Orlando, Fiorenza; Kamysz, E; Provinciali, M; Muzzonigro, Giovanni; Guerrieri, Mario; Giacometti, Andrea
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/158734
Citazioni:
In vitro and in vivo effects of sub-MICs of pexiganan and imipenem on Pseudomonas aeruginosa adhesion and biofilm development / Cirioni, O., Silvestri, C., Ghiselli, R., Kamysz, W., Minardi, D., Castelli, P., Orlando, F., Kamysz, E., Provinciali, M., Muzzonigro, G., Guerrieri, M., Giacometti, A.. - In: LE INFEZIONI IN MEDICINA. - ISSN 1124-9390. - STAMPA. - 21:(2013), pp. 287-295.
Abstract:
An in vitro and in vivo study was performed to quantify adhesion and biofilm formation ability of Pseudomonas aeruginosa slime producer under the effect of sub-minimal inhibitory concentrations (MICs) of pexiganan and imipenem. To evaluate adherence, squares of ureteral stents were placed in s...ix-well tissue-culture plates containing 6 ml of a cell suspension grown in the presence of sub- MICs of study antibiotics. To evaluate biofilm formation sterilized squares were placed in six-well tissue culture plates containing 6 ml of triptic soy broth (TSB) supplemented with 0.25% of glucose and the respective amount of antibiotic. For in vivo study a biofilm infection rat model was performed. The study included an uninfected control group to evaluate the sterility of surgical procedure, a group infected with a slime-producer P. aeruginosa strain not previously treated with antibiotics and two groups infected with the strain previously treated with imipenem or pexiganan. Adherence and biofilm in vitro formation was strongly affected by pre-treatment with pexiganan and imipenem, with the latter being the more effective antibiotic. The in vivo results showed a reduction in bacterial load on the ureteral stent tissue of the pre-treated strain. Differently, urine cultures showed no differences in bacterial growth for the pre-treated strain showing that it retained its ability to cause infection. This study suggests that sub-MIC imipenem and pexiganan could be a good strategy to target the adhesion process during the infection cycle.
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Interindividual and intra-individual variabilities of Darunavir and Ritonavir plasma trough concentrations in multidrug experienced HIV patients receiving salvage regimens.
Anno: 2013
Rivista: THERAPEUTIC DRUG MONITORING
Autori/autrici: Baroncelli, S; Villani, P; Galluzzo, Cm; Cavalli, A; Volpe, A; Francisci, D; Vivarelli, A; Sozio, F; Tedeschi, S; Cirioni, Oscar; Sighinolfi, L; Cusato, M; Pirillo, Mf; Weimer, Le; Fragola, V; Parruti, G; Regazzi, M; Floridia, M.
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/156117
Citazioni:
Interindividual and intra-individual variabilities of Darunavir and Ritonavir plasma trough concentrations in multidrug experienced HIV patients receiving salvage regimens / Baroncelli, S., Villani, P., Galluzzo, C.m., Cavalli, A., Volpe, A., Francisci, D., Vivarelli, A., Sozio, F., Tedeschi, S., Cirioni, O., Sighinolfi, L., Cusato, M., Pirillo, M.f., Weimer, L.e., Fragola, V., Parruti, G., Regazzi, M., Floridia, M.. - In: THERAPEUTIC DRUG MONITORING. - ISSN 0163-4356. - STAMPA. - 35:(2013), pp. 785-790.

No evidence of autoimmune disorders in antiretroviral-experienced HIV-1 infected subjects after long-term treatment with raltegravir.
Anno: 2013
Rivista: ANTIVIRAL THERAPY
Autori/autrici: Baroncelli, S; Mezzaroma, I; Fantauzzi, A; Galluzzo, Mc; Degli Antoni, A; Vullo, V; Francisci, D; Ladisa, N; Vivarelli, A; Cirioni, Oscar; Sighinolfi, L; Weimer, Le; Fragola, V; Fidanza, R; Cara, A; Palmisano, L.
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/156113
Citazioni:
No evidence of autoimmune disorders in antiretroviral-experienced HIV-1 infected subjects after long-term treatment with raltegravir / Baroncelli, S., Mezzaroma, I., Fantauzzi, A., Galluzzo, M.c., Degli Antoni, A., Vullo, V., Francisci, D., Ladisa, N., Vivarelli, A., Cirioni, O., Sighinolfi, L., Weimer, L.e., Fragola, V., Fidanza, R., Cara, A., Palmisano, L.. - In: ANTIVIRAL THERAPY. - ISSN 1359-6535. - STAMPA. - 18:(2013), pp. 321-327.

Antimicrobial properties of distinctin in an experimental model of MRSA-infected wounds.
Anno: 2012
Rivista: EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
Autori/autrici: Simonetti, Oriana; Cirioni, Oscar; Ghiselli, Roberto; Goteri, Gaia; Orlando, F.; Monfregola, L.; De Luca, S.; Zizzi, Antonio; Silvestri, C.; Veglia, G.; Giacometti, Andrea; Guerrieri, Mario; Offidani, Annamaria; Scaloni, A.
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/73263
Citazioni:
Antimicrobial properties of distinctin in an experimental model of MRSA-infected wounds / Simonetti, O., Cirioni, O., Ghiselli, R., Goteri, G., Orlando, F., Monfregola, L., De Luca, S., Zizzi, A., Silvestri, C., Veglia, G., Giacometti, A., Guerrieri, M., Offidani, A., Scaloni, A.. - In: EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES. - ISSN 0934-9723. - STAMPA. - 31:11(2012), pp. 3047-3055. [10.1007/s10096-012-1663-1]
Abstract:
The aim of this study was to evaluate the efficacy of distinctin in the management of cutaneous methicillin-resistant Staphylococcus aureus (MRSA) wound infections in an experimental mouse model. Wounds, made in the panniculus carnosus of BALB/c mice, were inoculated with 5×107 colony-forming ...units (CFU) of MRSA. Mice were treated with topical distinctin (1 mg/kg of body weight), topical teicoplanin (7 mg/kg of body weight), intraperitoneal teicoplanin (7 mg/kg of body weight); topical teicoplanin and daily intraperitoneal teicoplanin; topical distinctin and daily intraperitoneal teicoplanin. Bacterial cultures of excised tissues and histological examination of microvessel density and of vascular endothelial growth factor (VEGF) expression were studied. It was found that topical distinctin combined with parenteral teicoplanin inhibited bacterial growth to levels comparable with those observed in uninfected animals. Wounded areas of animals treated with distinctin were characterized by a more mature granulation tissue, with a more organized and denser type of connective tissue, compared to mice treated only with teicoplanin. Treatment with topical distinctin had a significant impact on VEGF expression and microvessel density. The combined use of distinctin with teicoplanin may be useful in the management of infected wounds by significantly inhibiting bacterial growth and accelerating the repair process.
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In vitro activity and in vivo efficacy of tigecycline alone and in combination with daptomycin and rifampin against Gram-positive cocci isolated from surgical wound infection.
Anno: 2012
Rivista: EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
Autori/autrici: Silvestri, Carmela; Cirioni, Oscar; Arzeni, D; Ghiselli, Roberto; Simonetti, Oriana; Orlando, F; Ganzetti, G; Staffolani, S; Brescini, L; Provinciali, M; Offidani, Annamaria; Guerrieri, Mario; Giacometti, Andrea
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/156119
Citazioni:
In vitro activity and in vivo efficacy of tigecycline alone and in combination with daptomycin and rifampin against Gram-positive cocci isolated from surgical wound infection / Silvestri, C., Cirioni, O., Arzeni, D., Ghiselli, R., Simonetti, O., Orlando, F., Ganzetti, G., Staffolani, S., Brescini, L., Provinciali, M., Offidani, A., Guerrieri, M., Giacometti, A.. - In: EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES. - ISSN 0934-9723. - STAMPA. - 31:8(2012), pp. 1759-1764. [10.1007/s10096-011-1498-1]

Use of direct renin inhibitor in an HIV positive subject
Anno: 2012
Rivista: LE INFEZIONI IN MEDICINA
Autori/autrici: Ucciferri, C; Brescini, L; Staffolania, S; Falasca, K; Cirioni, O; Giacometti, A; Vecchiet, J
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/294488
Citazioni:
Use of direct renin inhibitor in an HIV positive subject / Ucciferri, C., Brescini, L., Staffolania, S., Falasca, K., Cirioni, O., Giacometti, A., Vecchiet, J.. - In: LE INFEZIONI IN MEDICINA. - ISSN 2532-8689. - (2012).

Virological failure at one year in triple-class experienced patients switching to raltegravir-based regimens is not predicted by baseline factors.
Anno: 2012
Rivista: INTERNATIONAL JOURNAL OF STD & AIDS
Autori/autrici: Bucciardini, R; D'Ettorre, G; Baroncelli, S; Ceccarelli, G; Parruti, G; Weimer, Le; Fragola, V; Galluzzo, Cm; Pirillo, Mf; Lucattini, S; Bellagamba, R; Francisci, D; Ladisa, N; Degli Antoni, A; Guaraldi, G; Manconi, Pe; Vullo, V; Preziosi, R; Cirioni, Oscar; Verucchi, G; Floridia, M.
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/156118
Citazioni:
Virological failure at one year in triple-class experienced patients switching to raltegravir-based regimens is not predicted by baseline factors / Bucciardini, R., D'Ettorre, G., Baroncelli, S., Ceccarelli, G., Parruti, G., Weimer, L.e., Fragola, V., Galluzzo, C.m., Pirillo, M.f., Lucattini, S., Bellagamba, R., Francisci, D., Ladisa, N., Degli Antoni, A., Guaraldi, G., Manconi, P.e., Vullo, V., Preziosi, R., Cirioni, O., Verucchi, G., et al.. - In: INTERNATIONAL JOURNAL OF STD & AIDS. - ISSN 0956-4624. - STAMPA. - 23:(2012), pp. 459-463.

Efficacy of Tigecycline and Rifampin Alone and in Combination against Enterococcus faecalis Biofilm Infection in a Rat Model of Ureteral Stent.
Anno: 2012
Rivista: JOURNAL OF SURGICAL RESEARCH
Autori/autrici: Minardi, D; Cirioni, O; Ghiselli, R; Silvestri, C; Mocchegiani, F; Gabrielli, E; D'Anzeo, G; Conti, A; Orlando, F; Rimini, M; Brescini, L; Guerrieri, M; Giacometti, A; Muzzonigro, G.
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/156116
Citazioni:
Efficacy of Tigecycline and Rifampin Alone and in Combination against Enterococcus faecalis Biofilm Infection in a Rat Model of Ureteral Stent / Minardi, D., Cirioni, O., Ghiselli, R., Silvestri, C., Mocchegiani, F., Gabrielli, E., D'Anzeo, G., Conti, A., Orlando, F., Rimini, M., Brescini, L., Guerrieri, M., Giacometti, A., Muzzonigro, G.. - In: JOURNAL OF SURGICAL RESEARCH. - ISSN 1095-8673. - STAMPA. - 176:(2012), pp. 1-6.

Tigecycline accelerates staphylococcal-infected burn wound healing through matrix metalloproteinase-9 modulation
Anno: 2012
Rivista: JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
Autori/autrici: Simonetti, O; Cirioni, O; Lucarini, G; Orlando, F; Ghiselli, R; Silvestri, C; Brescini, L; Rocchi, M; Provinciali, M; Guerrieri, M; Di Primio, R; Giacometti, A; Offidani, A
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/262154
Citazioni:
Tigecycline accelerates staphylococcal-infected burn wound healing through matrix metalloproteinase-9 modulation / Simonetti, O., Cirioni, O., Lucarini, G., Orlando, F., Ghiselli, R., Silvestri, C., Brescini, L., Rocchi, M., Provinciali, M., Guerrieri, M., Di Primio, R., Giacometti, A., Offidani, A.. - In: JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY. - ISSN 1460-2091. - ELETTRONICO. - 67:1(2012), pp. 191-201. [10.1093/jac/dkr440]
Abstract:
We investigated the in vivo efficacy of tigecycline, a new glycylcycline (a tetracycline derivative), in the management of methicillin-resistant Staphylococcus aureus (MRSA)-infected experimental surgical wounds in rats. The main outcome measures were quantitative bacterial culture, histological exa...mination and immunohistochemical expression of matrix metalloproteinase-9 (MMP-9) and collagen IV.
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S-thanatin in vitro prevents colistin resistance and improves its efficacy in an animal model of Pseudomonas aeruginosa sepsis.
Anno: 2011
Rivista: PEPTIDES
Autori/autrici: Cirioni, Oscar; Wu, G; Li, L; Orlando, F; Silvestri, C; Ghiselli, Roberto; Shen, Z; Gabrielli, E; Brescini, L; Lezoche, Giovanni; Provinciali, M; Guerrieri, Mario; Giacometti, Andrea
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/55646
Citazioni:
S-thanatin in vitro prevents colistin resistance and improves its efficacy in an animal model of Pseudomonas aeruginosa sepsis / Cirioni, Oscar; Wu, G; Li, L; Orlando, F; Silvestri, C; Ghiselli, Roberto; Shen, Z; Gabrielli, E; Brescini, L; Lezoche, Giovanni; Provinciali, M; Guerrieri, Mario; Giacometti, Andrea. - In: PEPTIDES. - ISSN 0196-9781. - 32:(2011), pp. 697-701.
Abstract:
An experimental study was performed to evaluate the interaction between s-thanatin and colistin both in vitro and in vivo, using two Pseudomonas aeruginosa strains with different patterns of susceptibilities. We evaluated whether selecting for colistin-resistant P. aeruginosa could be prevented in v...itro by combining colistin with s-thanatin. The strains were serially exposed in broth to twofold stepwise increasing concentrations of colistin alone or in combination with a fixed concentration [0.25× minimum inhibitory concentration (MIC)] of s-thanatin. We also performed an in vitro synergy study. For in vivo studies, a mouse model of Pseudomonas sepsis has been used. Main outcome measures were lethality and quantitative blood cultures. Exposure to colistin alone gradually selected for Pseudomonas strains with an increased MIC. In vitro studies, s-thanatin showed a positive interaction with colistin, and was able to prevent its resistance. In vivo studies, s-thanatin combined with colistin exhibited the highest efficacy on all main outcome measurements. These results highlight the potential usefulness of this combination and provide a future therapeutic alternative in severe Pseudomonas infections.
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Vitamin E improves the in vivo efficacy of tigecycline and daptomycin in an animal model of wounds infected with meticillin-resistant Staphylococcus aureus.J Med Microbiol. 2011 Dec;60(Pt 12):1806-12
Anno: 2011
Rivista: JOURNAL OF MEDICAL MICROBIOLOGY
Autori/autrici: Provinciali, M; Cirioni, Oscar; Orlando, F; Pierpaoli, E; Barucca, A; Silvestri, C; Ghiselli, Roberto; Scalise, Alessandro; Brescini, L; Guerrieri, Mario; Giacometti, Andrea
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/65127
Citazioni:
Vitamin E improves the in vivo efficacy of tigecycline and daptomycin in an animal model of wounds infected with meticillin-resistant Staphylococcus aureus.J Med Microbiol. 2011 Dec;60(Pt 12):1806-12 / Provinciali, M., Cirioni, O., Orlando, F., Pierpaoli, E., Barucca, A., Silvestri, C., Ghiselli, R., Scalise, A., Brescini, L., Guerrieri, M., Giacometti, A.. - In: JOURNAL OF MEDICAL MICROBIOLOGY. - ISSN 0022-2615. - 60:(2011), pp. 1806-1812.

In vitro activity and in vivo efficacy of the saponin diosgenyl 2-amino-2-deoxy-β-D-glucopyranoside hydrochloride (HSM1) alone and in combination with daptomycin and vancomycin against Gram-positive cocci.
Anno: 2011
Rivista: JOURNAL OF MEDICAL MICROBIOLOGY
Autori/autrici: Cirioni, Oscar; Myszka, H; Dawgul, M; Ghiselli, Roberto; Orlando, F; Silvestri, C; Brescini, L; Kamysz, W; Guerrieri, M; Giacometti, Andrea
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/65143
Citazioni:
In vitro activity and in vivo efficacy of the saponin diosgenyl 2-amino-2-deoxy-β-D-glucopyranoside hydrochloride (HSM1) alone and in combination with daptomycin and vancomycin against Gram-positive cocci / Cirioni, O., Myszka, H., Dawgul, M., Ghiselli, R., Orlando, F., Silvestri, C., Brescini, L., Kamysz, W., Guerrieri, M., Giacometti, A.. - In: JOURNAL OF MEDICAL MICROBIOLOGY. - ISSN 0022-2615. - 60:(2011), pp. 1337-1343.

Protective Effect of Citropin 1.1 and Tazobactam-Piperacillin Against Oxidative Damage and Lethality in Mice Models of Gram-Negative Sepsis.
Anno: 2011
Rivista: JOURNAL OF SURGICAL RESEARCH
Autori/autrici: Ghiselli, Roberto; Silvestri, C; Cirioni, Oscar; Kamysz, W; Orlando, F; Calcinari, A; Kamysz, E; Casteletti, S; Rimini, M; Tocchini, M; Giacometti, Andrea; Guerrieri, Mario
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/56276
Citazioni:
Protective Effect of Citropin 1.1 and Tazobactam-Piperacillin Against Oxidative Damage and Lethality in Mice Models of Gram-Negative Sepsis / Ghiselli, R., Silvestri, C., Cirioni, O., Kamysz, W., Orlando, F., Calcinari, A., Kamysz, E., Casteletti, S., Rimini, M., Tocchini, M., Giacometti, A., Guerrieri, M.. - In: JOURNAL OF SURGICAL RESEARCH. - ISSN 0022-4804. - 171:(2011), pp. 726-733.
Abstract:
2010 Apr 20. [Epub ahead of print]

Effect of the combination of clarithromycin and amikacin on Pseudomonas aeruginosa biofilm in a animal model of ureteral stent infection.
Anno: 2011
Rivista: JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
Autori/autrici: Cirioni, Oscar; Ghiselli, R; Silvestri, C; Minardi, Daniele; Gabrielli, E; Orlando, F; Rimini, M; Brescini, L; Muzzonigro, G; Guerrieri, M; Giacometti, Andrea
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/55640
Citazioni:
Effect of the combination of clarithromycin and amikacin on Pseudomonas aeruginosa biofilm in a animal model of ureteral stent infection / Cirioni, O., Ghiselli, R., Silvestri, C., Minardi, D., Gabrielli, E., Orlando, F., Rimini, M., Brescini, L., Muzzonigro, G., Guerrieri, M., Giacometti, A.. - In: JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY. - ISSN 0305-7453. - 66:(2011), pp. 1318-1323.

Vitamin E improves the in vivo efficacy of tigecycline and daptomycin in an animal model of wounds infected with methicillin resistant Staphylococcus aureus.
Anno: 2011
Rivista: JOURNAL OF MEDICAL MICROBIOLOGY
Autori/autrici: Provinciali, M; Cirioni, Oscar; Orlando, F; Pierpaoli, E; Barucca, A; Silvestri, C; Ghiselli, R; Scalise, Alessandro; Brescini, L; Guerrieri, M; Giacometti, Andrea
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/63618
Citazioni:
Vitamin E improves the in vivo efficacy of tigecycline and daptomycin in an animal model of wounds infected with methicillin resistant Staphylococcus aureus / Provinciali, M., Cirioni, O., Orlando, F., Pierpaoli, E., Barucca, A., Silvestri, C., Ghiselli, R., Scalise, A., Brescini, L., Guerrieri, M., Giacometti, A.. - In: JOURNAL OF MEDICAL MICROBIOLOGY. - ISSN 0022-2615. - 60(Pt 12):(2011), pp. 1806-1812.

Effectiveness of antimicrobial photodynamic therapy with a single treatment of RLP068/CI in an experimental model of Staphylococcus aureus wound infection
Anno: 2011
Rivista: BRITISH JOURNAL OF DERMATOLOGY
Autori/autrici: Simonetti, Oriana; Cirioni, Oscar; Orlando, F.; Alongi, C.; Lucarini, Guendalina; Silvestri, C.; Zizzi, A.; Fanteti, L.; Roncucci, G.; Giacometti, Andrea; Offidani, Annamaria; Provinciali, M.
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/55561
Citazioni:
Effectiveness of antimicrobial photodynamic therapy with a single treatment of RLP068/CI in an experimental model of Staphylococcus aureus wound infection / Simonetti, O., Cirioni, O., Orlando, F., Alongi, C., Lucarini, G., Silvestri, C., Zizzi, A., Fanteti, L., Roncucci, G., Giacometti, A., Offidani, A., Provinciali, M.. - In: BRITISH JOURNAL OF DERMATOLOGY. - ISSN 0007-0963. - 164(5) 2011:(2011), pp. 987-995.

Vitamin E supplementation in old mice induces antimicrobial activity and improves the efficacy of daptomycin in an animal model of wounds infected with methicillin resistant Staphylococcus aureus.
Anno: 2011
Rivista: JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
Autori/autrici: Pierpaoli, E; Cirioni, Oscar; Barucca, A; Orlandoni, Francesco; Silvestri, Carmela; Giacometti, Andrea; Provinciali, M.
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/63619
Citazioni:
Vitamin E supplementation in old mice induces antimicrobial activity and improves the efficacy of daptomycin in an animal model of wounds infected with methicillin resistant Staphylococcus aureus / Pierpaoli, E., Cirioni, O., Barucca, A., Orlandoni, F., Silvestri, C., Giacometti, A., Provinciali, M.. - In: JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY. - ISSN 0305-7453. - STAMPA. - 66:9(2011), pp. 2184-2185. [10.1093/jac/dkr254]

Vitamin E improves the in vivo efficacy of tigecycline and daptomycin in an animal model of wounds infected with meticillin-resistant Staphylococcus aureus.
Anno: 2011
Rivista: JOURNAL OF MEDICAL MICROBIOLOGY
Autori/autrici: Provinciali, M; Cirioni, Oscar; Orlandoni, Francesco; Pierpaoli, E; Barucca, A; Silvestri, Carmela; Ghiselli, Roberto; Scalise, Alessandro; Brescini, Lucia; Guerrieri, Mario; Giacometti, Andrea
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/73906
Citazioni:
Vitamin E improves the in vivo efficacy of tigecycline and daptomycin in an animal model of wounds infected with meticillin-resistant Staphylococcus aureus / Provinciali, M., Cirioni, O., Orlandoni, F., Pierpaoli, E., Barucca, A., Silvestri, C., Ghiselli, R., Scalise, A., Brescini, L., Guerrieri, M., Giacometti, A.. - In: JOURNAL OF MEDICAL MICROBIOLOGY. - ISSN 0022-2615. - 60:(2011). [10.1099/jmm.0.032516-0]

In vitro activity of the lipopeptide PAL-Lys-Lys-NH2, alone and in combination with antifungal agents, against clinica isolates of Candida spp
Anno: 2011
Rivista: PEPTIDES
Autori/autrici: Kamysz, E; Simonetti, Oriana; Cirioni, Oscar; Arzeni, D; Ganzetti, G; Campanati, Anna; Giacometti, Andrea; Gabrielli, E; Silvestri, C; Kamysz, W; Offidani, Annamaria; Barchiesi, Francesco
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/55657
Citazioni:
In vitro activity of the lipopeptide PAL-Lys-Lys-NH2, alone and in combination with antifungal agents, against clinica isolates of Candida spp / Kamysz, E., Simonetti, O., Cirioni, O., Arzeni, D., Ganzetti, G., Campanati, A., Giacometti, A., Gabrielli, E., Silvestri, C., Kamysz, W., Offidani, A., Barchiesi, F.. - In: PEPTIDES. - ISSN 0196-9781. - 32:(2011), pp. 99-103.
Abstract:
2010 Nov 3. [Epub ahead of print]

Lipopeptide Laur-CKK-NH2 dimer preserves daptomycin susceptibility and enhances its activity against Enterococcus faecalis.
Anno: 2011
Rivista: JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
Autori/autrici: Cirioni, Oscar; Kamysz, E; Ghiselli, Roberto; Kamysz, W; Silvestri, Carmela; Orlandoni, Francesco; Rimini, M; Brescini, Lucia; Gabrielli, E; Marchionni, E; Rocchi, M; Provinciali, M; Guerrieri, Mario; Giacometti, Andrea
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/76523
Citazioni:
Lipopeptide Laur-CKK-NH2 dimer preserves daptomycin susceptibility and enhances its activity against Enterococcus faecalis / Cirioni, O., Kamysz, E., Ghiselli, R., Kamysz, W., Silvestri, C., Orlandoni, F., Rimini, M., Brescini, L., Gabrielli, E., Marchionni, E., Rocchi, M., Provinciali, M., Guerrieri, M., Giacometti, A.. - In: JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY. - ISSN 0305-7453. - STAMPA. - 66:4(2011), pp. 859-862. [10.1093/jac/dkr001]
Abstract:
OBJECTIVES: An experimental study was performed to evaluate both in vitro and in vivo the kind of interaction between the Laur-CKK-NH2 dimer and daptomycin using two Enterococcus faecalis strains with different patterns of susceptibilities. METHODS: We evaluated whether selection for daptomycin-r...esistant E. faecalis could be prevented in vitro by combining daptomycin with the Laur-CKK-NH2 dimer. The strains were serially exposed in broth to 2-fold stepwise increasing concentrations of daptomycin alone or in combination with a fixed concentration (0.25×MIC) of the Laur-CKK-NH2 dimer. We also performed an in vitro synergy study. For in vivo studies, a mouse model of enterococcal sepsis was used. RESULTS: In vitro experiments: exposure to daptomycin alone gradually selected for enterococci with increased MICs; and the Laur-CKK-NH2 dimer showed a positive interaction with daptomycin and was able to prevent the resistance. In vivo experiments: the main outcome measures were lethality and quantitative blood cultures; and the Laur-CKK-NH2 dimer combined with daptomycin exhibited the highest efficacy for all main outcome measurements. CONCLUSIONS: These results highlight the potential usefulness of combining daptomycin with the Laur-CKK-NH2 dimer. The combination provides a future therapeutic alternative for the treatment of enterococcal severe infections.
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Daptomycin and Rifampin Alone and in Combination Prevent Vascular Graft Biofilm Formation and Emergence of Antibiotic Resistance in a Subcutaneous Rat Pouch Model of Staphylococcal Infection.
Anno: 2010
Rivista: EUROPEAN JOURNAL OF VASCULAR AND ENDOVASCULAR SURGERY
Autori/autrici: Cirioni, Oscar; Mocchegiani, Federico; Ghiselli, Roberto; Silvestri, Carmela; Gabrielli, E; Marchionni, E; Orlandoni, Francesco; Nicolini, D; Risaliti, A; Giacometti, Andrea
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/54029
Citazioni:
Daptomycin and Rifampin Alone and in Combination Prevent Vascular Graft Biofilm Formation and Emergence of Antibiotic Resistance in a Subcutaneous Rat Pouch Model of Staphylococcal Infection / Cirioni, Oscar; Mocchegiani, Federico; Ghiselli, Roberto; Silvestri, Carmela; Gabrielli, E; Marchionni, E; Orlandoni, Francesco; Nicolini, D; Risaliti, A; Giacometti, Andrea. - In: EUROPEAN JOURNAL OF VASCULAR AND ENDOVASCULAR SURGERY. - ISSN 1078-5884. - 40:(2010), pp. 817-822.
Abstract:
OBJECTIVE: To investigate the efficacy of daptomycin and rifampin either alone or in combination in preventing prosthesis biofilm in a rat model of staphylococcal vascular graft infection. DESIGN: Prospective, randomised, controlled animal study. MATERIALS: Graft infections were established in... the back subcutaneous tissue of adult male Wistar rats by implantation of Dacron prostheses followed by topical inoculation with 2×10(7) colony forming units of Staphylococcus aureus, strain Smith diffuse. METHODS: The study included a control group, a contaminated group that did not receive any antibiotic prophylaxis and three contaminated groups that received intra-peritoneal daptomycin, rifampin-soaked graft and daptomycin plus rifampin-soaked graft, respectively. Each group included 15 animals. The infection burden was evaluated by using sonication and quantitative agar culture. Moreover, an in vitro antibiotic susceptibility assay for S. aureus biofilms was performed to elucidate the same activity. RESULTS: When tested alone, daptomycin and rifampin showed good efficacies. Their combination showed efficacies significantly higher than that of each single compound. The in vitro studies showed that minimum inhibitory concentration and minimum bactericidal concentration values for daptomycin were lower in presence of rifampin. Daptomycin prevented the emergence of rifampin resistance. CONCLUSION: Daptomycin is an important candidate for prevention of staphylococcal biofilm-related infection and rifampin could serve as an interesting anti-staphylococcal antibiotic enhancer.
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S-thanatin enhances the efficacy of tigecycline in an experimental rat model of polymicrobial peritonitis.
Anno: 2010
Rivista: PEPTIDES
Autori/autrici: Cirioni, Oscar; Wu, G; Li, L; Orlando, F; Silvestri, C; Ghiselli, Roberto; Shen, Z; Scalise, Alessandro; Gabrielli, E; Scuppa, D; Romiti, C; Provinciali, M; Guerrieri, Mario; Giacometti, Andrea
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/27308
Citazioni:
S-thanatin enhances the efficacy of tigecycline in an experimental rat model of polymicrobial peritonitis / Cirioni, Oscar; Wu, G; Li, L; Orlando, F; Silvestri, C; Ghiselli, Roberto; Shen, Z; Scalise, Alessandro; Gabrielli, E; Scuppa, D; Romiti, C; Provinciali, M; Guerrieri, Mario; Giacometti, Andrea. - In: PEPTIDES. - ISSN 0079-0753. - 31:(2010), pp. 1231-1236.

Tollerabilità metabolica e durevolezza degli schemi antiretrovirali comprendenti nevirapina (Durability and tolerability of long-term nevirapine-based HAART).
Anno: 2010
Rivista: LE INFEZIONI IN MEDICINA
Autori/autrici: Giacometti, Andrea; Butini, L; Cirioni, Oscar; Costantini, Andrea; Montroni, Maria; Scalise, Giorgio
Lingua: ITA
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/39578
Citazioni:
Tollerabilità metabolica e durevolezza degli schemi antiretrovirali comprendenti nevirapina (Durability and tolerability of long-term nevirapine-based HAART) / Giacometti, A., Butini, L., Cirioni, O., Costantini, A., Montroni, M., Scalise, G.. - In: LE INFEZIONI IN MEDICINA. - ISSN 1124-9390. - 11:(2010), pp. 20-26.
Abstract:
Efficacy and tolerability of nevirapine-based HAART regimens were retrospectively evaluated. HIV-1-infected patients were included if they had been receiving a NVPcontaining HAART regimen for at least 60 months, regardless of the reason for its initiation. A total of 82 patients were included in thi...s study. The median follow-up time period was 96 months (range 64-120). At the time of starting NVP-based therapy, 31.7% (26 patients) were antiretroviral-naïve, while 68.3% (56 patients) switched to an NVP-based strategy because of intolerance to the prior regimen, failure of a previous regimen or for simplification purposes. Coinfection with hepatitis B or C viruses was present in 26.8% of the patients (n=22). The most frequent nucleoside analogue backbone was zidovudine/lamivudine (52.4%) followed by stavudine/lamivudine (15.8%). A protease inhibitor was concomitantly administered in 2.4% of cases. To investigate the tolerability, we report the results separately on the basis of the presence/absence of HBV and/or HCV coinfection. Coinfected patients displayed significantly higher baseline liver enzymes than non-coinfected patients. Nevertheless, ALT levels showed a constant decrease over time in coinfected patients. Overall, median triglycerides, HDL and total cholesterol values tended to be lower in coinfected patients than in the remaining subjects. In particular, there was a significant decrease in triglyceride values in both groups and, concomitantly, a slight increase in total cholesterol. On the other hand, HDL cholesterol demonstrated a progressive increase. Finally, no change was found in glucose plasma levels, which always remained within normal ranges. In conclusion, no long-term toxicities associated with the use of nevirapine beyond five years were documented in our cohort of patients, even in coinfected patients. Long-term exposure to nevirapine was associated with optimal HIV suppression and favourable lipidic and glucidic profiles.
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Durability and tolerability of long-term nevirapine-based HAART.
Anno: 2010
Rivista: LE INFEZIONI IN MEDICINA
Autori/autrici: Giacometti, Andrea; Butini, L; Cirioni, O; Costantini, A; Montroni, M; Scalise, G.
Lingua: ITA
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/54026
Citazioni:
Durability and tolerability of long-term nevirapine-based HAART / Giacometti, A., Butini, L., Cirioni, O., Costantini, A., Montroni, M., Scalise, G.. - In: LE INFEZIONI IN MEDICINA. - ISSN 1124-9390. - 18:(2010), pp. 20-26.

Daptomycin and rifampin aloneand in combination prevent vascular graft biofilm formation and emergence ofantibiotic resistance in a subcutaneous rat pouch model of staphylococcalinfection
Anno: 2010
Rivista: EUROPEAN JOURNAL OF VASCULAR AND ENDOVASCULAR SURGERY
Autori/autrici: Cirioni, Oscar; Mocchegiani, Federico; Ghiselli, Roberto; Silvestri, C; Gabrielli, E; Marchionni, E; Orlando, F; Nicolini, D; Risaliti, A; Giacometti, Andrea
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/55643
Citazioni:
Daptomycin and rifampin aloneand in combination prevent vascular graft biofilm formation and emergence ofantibiotic resistance in a subcutaneous rat pouch model of staphylococcalinfection / Cirioni, O., Mocchegiani, F., Ghiselli, R., Silvestri, C., Gabrielli, E., Marchionni, E., Orlando, F., Nicolini, D., Risaliti, A., Giacometti, A.. - In: EUROPEAN JOURNAL OF VASCULAR AND ENDOVASCULAR SURGERY. - ISSN 1078-5884. - 40:(2010), pp. 817-822.

Tollerabilità metabolica e durevolezza degli schemi antiretrovirali comprendenti nevirapina.
Anno: 2010
Rivista: LE INFEZIONI IN MEDICINA
Autori/autrici: Giacometti, Andrea; Butini, L; Cirioni, O; Costantini, Andrea; Montroni, Maria; Scalise, G.
Lingua: ITA
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/26272
Citazioni:
Tollerabilità metabolica e durevolezza degli schemi antiretrovirali comprendenti nevirapina / Giacometti, A., Butini, L., Cirioni, O., Costantini, A., Montroni, M., Scalise, G.. - In: LE INFEZIONI IN MEDICINA. - ISSN 1124-9390. - 1:(2010), pp. 20-26.

OpuC--an ABC transporter that is associated with Staphylococcus aureus pathogenesis.
Anno: 2009
Rivista: INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS
Autori/autrici: Kiran, Md; Akiyoshi, De; Giacometti, Andrea; Cirioni, O; Scalise, G; Balaban, N.
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/54024
Citazioni:
OpuC--an ABC transporter that is associated with Staphylococcus aureus pathogenesis / Kiran, Md; Akiyoshi, De; Giacometti, Andrea; Cirioni, O; Scalise, G; Balaban, N.. - In: INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS. - ISSN 0391-3988. - 32:(2009), pp. 600-610.

Therapeutic efficacy of buforin II and rifampin in a rat model of Acinetobacter baumannii sepsis.
Anno: 2009
Rivista: CRITICAL CARE MEDICINE
Autori/autrici: Cirioni, Oscar; Silvestri, C; Ghiselli, Roberto; Orlando, F; Riva, A; Gabrielli, E; Mocchegiani, Federico; Cianforlini, N; Trombettoni, Mm; Saba, V; Scalise, Giorgio; Giacometti, Andrea
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/53927
Citazioni:
Therapeutic efficacy of buforin II and rifampin in a rat model of Acinetobacter baumannii sepsis / Cirioni, Oscar; Silvestri, C; Ghiselli, Roberto; Orlando, F; Riva, A; Gabrielli, E; Mocchegiani, Federico; Cianforlini, N; Trombettoni, Mm; Saba, V; Scalise, Giorgio; Giacometti, Andrea. - In: CRITICAL CARE MEDICINE. - ISSN 0090-3493. - 37:(2009), pp. 1403-1407.
Abstract:
OBJECTIVE: To investigate the efficacy of buforin II and rifampin in an experimental rat model of Acinetobacter baumannii sepsis. DESIGN: Prospective, randomized, controlled animal study. SETTING: Research laboratory in a university hospital. SUBJECTS: Adult male Wistar rats. INTERVENTIONS: ... The animals received intraperitoneally 1 mL saline containing 2 x 10 colony forming units of A. baumannii ATCC 19606 (model i) or the multiresistant strain (model ii). Immediately after bacterial challenge, animals received intravenously a single dose of isotonic sodium chloride solution (control groups C1 and C2), 1 mg/kg of buforin II, 10 mg/kg of rifampin, and 1 mg/kg of buforin II plus 10 mg/kg of rifampin, respectively. MEASUREMENTS AND MAIN RESULTS: Lethality, bacterial growth in blood and tissue burden, endotoxin, interleukin-6, and tumor necrosis factor-alpha concentrations in plasma. Buforin II showed good antimicrobial activity and achieved a significant reduction of plasma endotoxin and cytokines concentration when compared with control and rifampin-treated groups. Combination among buforin II proved to be the most effective treatment in reducing all variables measured. CONCLUSION: In an experimental model, buforin II and rifampin might have a potential role in the treatment of severe infections due to A. baumannii.
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In vitro activity of the lipopeptide derivative (Pal-Lys-Lys-NH), alone and in combination with antifungal agents, against clinical isolates of dermatophytes.
Anno: 2009
Rivista: BRITISH JOURNAL OF DERMATOLOGY
Autori/autrici: Simonetti, Oriana; Arzeni, D; Ganzetti, G; Silvestri, C; Cirioni, Oscar; Gabrielli, E; Castelletti, S; Kamysz, W; Kamysz, E; Scalise, G; Offidani, Annamaria; Barchiesi, Francesco
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/55663
Citazioni:
In vitro activity of the lipopeptide derivative (Pal-Lys-Lys-NH), alone and in combination with antifungal agents, against clinical isolates of dermatophytes / Simonetti, O., Arzeni, D., Ganzetti, G., Silvestri, C., Cirioni, O., Gabrielli, E., Castelletti, S., Kamysz, W., Kamysz, E., Scalise, G., Offidani, A., Barchiesi, F.. - In: BRITISH JOURNAL OF DERMATOLOGY. - ISSN 0007-0963. - 16:(2009), pp. 249-252.

Antimicrobial peptides (Temporin A and Iseganan IB-367): effect on the cysticerci of Taenia crassiceps.
Anno: 2009
Rivista: MOLECULAR AND BIOCHEMICAL PARASITOLOGY
Autori/autrici: Landa, A; Jiménez, L; Willms, K; Jiménez García, Lf; Lara Martínez, R; Robert, L; Cirioni, Oscar; Barańska Rybak, W; Kamysz, W.
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/55654
Citazioni:
Antimicrobial peptides (Temporin A and Iseganan IB-367): effect on the cysticerci of Taenia crassiceps / Landa, A., Jiménez, L., Willms, K., Jiménez García, L.f., Lara Martínez, R., Robert, L., Cirioni, O., Barańska Rybak, W., Kamysz, W.. - In: MOLECULAR AND BIOCHEMICAL PARASITOLOGY. - ISSN 0166-6851. - 164:(2009), pp. 126-130.

In vitro susceptibility of dermatophytes to conventional and alternative antifungal agents.
Anno: 2009
Rivista: MEDICAL MYCOLOGY
Autori/autrici: Barchiesi, Francesco; Silvestri, Carmela; Arzeni, D.; Ganzetti, Giulia; Castelletti, S.; Simonetti, Oriana; Cirioni, Oscar; Kamysz, W.; Kamysz, E.; Spreghini, E.; Abruzzetti, A.; Riva, A.; Offidani, Annamaria; Giacometti, Andrea; Scalise, Giorgio
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/50175
Citazioni:
In vitro susceptibility of dermatophytes to conventional and alternative antifungal agents / Barchiesi, F., Silvestri, C., Arzeni, D., Ganzetti, G., Castelletti, S., Simonetti, O., Cirioni, O., Kamysz, W., Kamysz, E., Spreghini, E., Abruzzetti, A., Riva, A., Offidani, A., Giacometti, A., Scalise, G.. - In: MEDICAL MYCOLOGY. - ISSN 1369-3786. - 47:(2009), pp. 321-326. [10.1080/13693780802641920]

In vitro activity of the lipopetide derivative (Pal-lys-lysNh2), alone and in combination with antifungal agents, against clinical isolates of dermatophytes
Anno: 2009
Rivista: BRITISH JOURNAL OF DERMATOLOGY
Autori/autrici: Simonetti, Oriana; Arzeni, D; Ganzetti, Giulia; Silvestri, C; Cirioni, Oscar; Gabrielli, E; Castelletti, S; Kamysz, W; Kamysz, E; Scalise, G; Offidani, Annamaria; Barchiesi, Francesco
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/35668
Citazioni:
In vitro activity of the lipopetide derivative (Pal-lys-lysNh2), alone and in combination with antifungal agents, against clinical isolates of dermatophytes / Simonetti, O., Arzeni, D., Ganzetti, G., Silvestri, C., Cirioni, O., Gabrielli, E., Castelletti, S., Kamysz, W., Kamysz, E., Scalise, G., Offidani, A., Barchiesi, F.. - In: BRITISH JOURNAL OF DERMATOLOGY. - ISSN 0007-0963. - 161(2):2(2009), pp. 249-252. [10.1111/j.1365-2133.2009.09166.x]

In vitro activity of Tachyplesin III alone and in combination with terbinafine against clinical isolates of dermatophytes.
Anno: 2009
Rivista: PEPTIDES
Autori/autrici: Simonetti, Oriana; Ganzetti, Giulia; Arzeni, D; Campanati, Anna; Marconi, B; Silvestri, Carmela; Cirioni, Oscar; Gabrielli, E; Lenci, I; Kamysz, W; Kamysz, E; Giacometti, Andrea; Scalise, G; Barchiesi, Francesco; Offidani, Annamaria
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/75915
Citazioni:
In vitro activity of Tachyplesin III alone and in combination with terbinafine against clinical isolates of dermatophytes / Simonetti, O., Ganzetti, G., Arzeni, D., Campanati, A., Marconi, B., Silvestri, C., Cirioni, O., Gabrielli, E., Lenci, I., Kamysz, W., Kamysz, E., Giacometti, A., Scalise, G., Barchiesi, F., Offidani, A.. - In: PEPTIDES. - ISSN 0196-9781. - 30:10(2009), pp. 1794-1797. [10.1016/j.peptides.2009.06.033]

Efficacy of the amphibian peptide distinctin in a neutropenic mouse model of staphylococcal sepsis
Anno: 2008
Rivista: CRITICAL CARE MEDICINE
Autori/autrici: Cirioni, O; Ghiselli, R; Orlando, F; Silvestri, C; DE LUCA, S; Salzano, Am; Mocchegiani, Federico; Saba, V; Scalise, G; Scaloni, A; Giacometti, Andrea
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/53925
Citazioni:
Efficacy of the amphibian peptide distinctin in a neutropenic mouse model of staphylococcal sepsis / Cirioni, O; Ghiselli, R; Orlando, F; Silvestri, C; DE LUCA, S; Salzano, Am; Mocchegiani, Federico; Saba, V; Scalise, G; Scaloni, A; Giacometti, Andrea. - In: CRITICAL CARE MEDICINE. - ISSN 0090-3493. - 36(9):(2008), pp. 2629-2633.
Abstract:
Abstract OBJECTIVE: To investigate the efficacy of distinctin in a neutropenic mouse model of staphylococcal sepsis. DESIGN: Prospective, randomized, and controlled animal study. SETTING: Research laboratory in a University Hospital. SUBJECTS: BALB/c male mice. INTERVENTIONS: Mice were ren...dered neutropenic by injecting cyclophosphamide (200 mg/kg of body weight/day) on days -4 and -2 preinfection. Infection was induced at time 0 by intraperitoneal injection of 1 x 10(9) colony forming units of the staphylococcal strain. For each model, all animals were randomized to receive intravenous isotonic sodium chloride solution, 1 mg/kg distinctin, and 10 mg/kg imipenem, 10 mg/kg vancomycin, 10 mg/kg teicoplanin or 10 mg/kg linezolid alone, or combined with 1 mg/kg distinctin. MEASUREMENTS AND MAIN RESULTS: Lethality, bacterial growth in blood and peritoneum, spleen, liver, and mesenteric lymph nodes. RESULTS: All combined regimen showed lower lethality rates than singly treated-groups. Distinctin plus vancomycin or teicoplanin exerted the lowest lethality rate. All regimens were significantly superior to controls at reducing blood, spleen, peritoneum, liver and mesenteric lymph node complex bacterial burdens, whereas all combined treated groups were higher effective than singly treated groups. CONCLUSION: Our data indicate that distinctin alone or combined with other antibiotics may be useful in treating severe staphylococcal infections.
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Efficacy of LL-37 and Granulocyte-Colony Stimulating Factor in a Neutropenic Murine Sepsis due to Pseudomonas aeruginosa
Anno: 2008
Rivista: SHOCK
Autori/autrici: Cirioni, Oscar; Ghiselli, Roberto; Tomasinsig, L; Orlando, F; Silvestri, C; Skerlavaj, B; Riva, A; Rocchi, M; Saba, V; Zanetti, M; Scalise, G; Giacometti, Andrea
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/50719
Citazioni:
Efficacy of LL-37 and Granulocyte-Colony Stimulating Factor in a Neutropenic Murine Sepsis due to Pseudomonas aeruginosa / Cirioni, Oscar; Ghiselli, Roberto; Tomasinsig, L; Orlando, F; Silvestri, C; Skerlavaj, B; Riva, A; Rocchi, M; Saba, V; Zanetti, M; Scalise, G; Giacometti, Andrea. - In: SHOCK. - ISSN 1073-2322. - 30:(2008), pp. 443-448.

BMAP-28 improves the efficacy of vancomycin in rat models of gram-positive cocci ureteral stent infection
Anno: 2008
Rivista: PEPTIDES
Autori/autrici: Orlando, F.; Ghiselli, Roberto; Cirioni, Oscar; Minardi, Daniele; Tomasinsig, L.; Mocchegiani, Federico; Silvestri, C.; Skerlavaj, B.; Riva, A.; Muzzonigro, Giovanni; Saba, V.; Scalise, G.; Zanetti, M.; Giacometti, Andrea
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/50142
Citazioni:
BMAP-28 improves the efficacy of vancomycin in rat models of gram-positive cocci ureteral stent infection / Orlando, F.; Ghiselli, Roberto; Cirioni, Oscar; Minardi, Daniele; Tomasinsig, L.; Mocchegiani, Federico; Silvestri, C.; Skerlavaj, B.; Riva, A.; Muzzonigro, Giovanni; Saba, V.; Scalise, G.; Zanetti, M.; Giacometti, Andrea. - In: PEPTIDES. - ISSN 0079-0753. - 29:(2008), pp. 1118-1123.

Suppression of biofilm related, device-associated infections by staphylococcal quorum sensing inhibitors.
Anno: 2008
Rivista: ESAO PROCEEDINGS
Autori/autrici: Kiran, Md; Giacometti, Andrea; Cirioni, Oscar; Balaban, N.
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/36436
Citazioni:
Suppression of biofilm related, device-associated infections by staphylococcal quorum sensing inhibitors / Kiran, Md; Giacometti, Andrea; Cirioni, Oscar; Balaban, N.. - In: ESAO PROCEEDINGS. - ISSN 0952-3391. - 31:(2008), pp. 761-770.

Tachyplesin III and granulocyte-colony stimulating factor enhance the efficacy of tazobactam/piperacillin in a neutropenic mouse model of polymicrobial peritonitis.
Anno: 2008
Rivista: PEPTIDES
Autori/autrici: Cirioni, O; Ghiselli, R; Kamysz, W; Orlando, F; Silvestri, C; Mocchegiani, Federico; Di, ; Matteo, F; Kamysz, E; Riva, A; Rocchi, M; Saba, V; Scalise, G; Giacometti, Andrea
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/53921
Citazioni:
Tachyplesin III and granulocyte-colony stimulating factor enhance the efficacy of tazobactam/piperacillin in a neutropenic mouse model of polymicrobial peritonitis / Cirioni, O; Ghiselli, R; Kamysz, W; Orlando, F; Silvestri, C; Mocchegiani, Federico; Di, ; Matteo, F; Kamysz, E; Riva, A; Rocchi, M; Saba, V; Scalise, G; Giacometti, Andrea. - In: PEPTIDES. - ISSN 0079-0753. - 29(1):(2008), pp. 31-38.

Suppression of biofilm related, device-associated infections by staphylococcal quorum sensing inhibitors.
Anno: 2008
Rivista: INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS
Autori/autrici: Kiran, Md; Giacometti, Andrea; Cirioni, O; Balaban, N.
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/29183
Citazioni:
Suppression of biofilm related, device-associated infections by staphylococcal quorum sensing inhibitors / Kiran, Md; Giacometti, Andrea; Cirioni, O; Balaban, N.. - In: INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS. - ISSN 0391-3988. - 31:(2008), pp. 761-770.