Comparative efficacy of topical versus systemic teicoplanin in experimental model of wound infections
Anno:
2008
Rivista: JOURNAL OF SURGICAL RESEARCH
Autori/autrici: Ghiselli, Roberto; Cirioni, Oscar; Giacometti, Andrea; Scalise, Alessandro; Simonetti, Oriana; Mocchegiani, Federico; Orlando, F.; Goteri, Gaia; DELLA VITTORIA, A.; Filosa, A.; Silvestri, C.; Offidani, Annamaria; Bertani, A.; Scalise, Giorgio; Saba, V.
Lingua: ENG
Classificazione IRIS: 1.1 Articolo in rivista
Scheda completa IRIS: https://iris.univpm.it/handle/11566/50141
Citazioni:Comparative efficacy of topical versus systemic teicoplanin in experimental model of wound infections / Ghiselli, R., Cirioni, O., Giacometti, A., Scalise, A., Simonetti, O., Mocchegiani, F., Orlando, F., Goteri, G., DELLA VITTORIA, A., Filosa, A., Silvestri, C., Offidani, A., Bertani, A., Scalise, G., Saba, V.. - In: JOURNAL OF SURGICAL RESEARCH. - ISSN 0022-4804. - 144:1(2008), pp. 74-81. [10.1016/j.jss.2007.02.051]
Abstract:most common cause of nosocomial infections and, typically,
gram-positive pathogens are involved.
Materials and methods. A mouse model was used to
investigate the efficacy of different methods for the
treatment of wound infections. A full thickness wound
was established on the back subcutaneous
...tissue of
adult male BALB/c mice. A small gauze was placed
over each wound and then inoculated with 5 107
colony-forming units of Staphylococcus aureus. The
study included a control group that did not receive
any treatment and four contaminated groups treated,
respectively, with: (1) drug-free Allevyn (Smith and
Nephew Healthcare, Yorkshire, United Kingdom), (2)
teicoplanin-soaked Allevyn, (3) drug-free Allevyn and
daily intraperitoneal teicoplanin (7 mg/kg) and, finally,
(4) teicoplanin-soaked Allevyn and daily intraperitoneal
teicoplanin (7 mg/kg). Main outcome measures
were quantitative bacterial culture, assessment
of vascular endothelial growth factor (VEGF) plasma
levels, histological examination with assessment of microvessel
density, and of VEGF expression in tissue
sections.
Results. Data analysis showed that strong inhibition
of bacterial growth was achieved in any group treated
with intraperitoneal teicoplanin. However, the highest
inhibition of bacterial growth was obtained in the
group that received teicoplanin-soaked Allevyn and
intraperitoneal teicoplanin. Histological examination
showed that each treatment modality was able to reduce
the delay in wound repair. The most effective
treatment appeared to be the local application of
teicoplanin-soaked hydro gel foam. The tissue effects
were associated with an increase in neovascularization
and VEGF expression by endothelial cells and
fibroblasts in the granulation tissue. Bacterial colonies
also were reduced, especially when teicoplanin
was given parenterally.
Conclusions. Soaking a hydro cellular foam with an
antistaphylococcal agents, such as teicoplanin, may be
useful for the management of infected wounds. © 2008
Elsevier Inc. All rights reserved.
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